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Relationship between apoptosis and the BH2 domain sequence of the VP5 peptide of infectious pancreatic necrosis virus

Relationship between apoptosis and the BH2 domain sequence of the VP5 peptide of infectious pancreatic necrosis virus



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Ortega S, C., Rodríguez S, S., Espinoza, J. C., Kuznar, J., Romero, A., & Enríquez, R. (2014). Relationship between apoptosis and the BH2 domain sequence of the VP5 peptide of infectious pancreatic necrosis virus. Revista MVZ Córdoba, 19(1), 3990-4002. https://doi.org/10.21897/rmvz.119

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PlumX
Cesar Ortega S
Sylvia Rodríguez S
Juan Carlos Espinoza
Juan Kuznar
Alex Romero
Ricardo Enríquez

ABSTRACT

Objective. To determine whether the level of apoptosis induced by infectious pancreatic necrosis virus (IPNV) is related to the amino acid sequence of the BH2 domain of the VP5 protein and the level of infectivity. Materials and methods. Three IPNV strains were used, the VP2 protein gene was amplified for genotyping and the VP5 sequence was also obtained. The infectivity of the strains was calculated using the viral titer obtained at 12, 24, 36 and 45 hpi in CHSE-214 cells. The percentage of apoptosis in infected cells was visualized by TUNEL assay and immunohistochemistry (caspase 3 detection). Results. The V70/06 and V33/98 strains corresponded to genotype Sp, while V112/06 to VR-299; the amino acid analysis of the V70/06 strain allows its classification as middle virulent strain and V33/98 and V112/06 strains as low virulent ones; infection with the V112/06 strain produced a lower viral titer (p<0.05). The VP5 gene of the 3 strains showed four homologous domains to Bcl-2, however, the BH2 domain was truncated in V70/06 and V33/98 (12 kDa), being complete (15kDa) in V112/06, which also showed the Trp155 residue, equivalent to Trp188 considered as a critical factor for the function of Bcl-2. The average apoptosis was below 12%, showing no differences between strains (p>0.05). Conclusions. The results showed that the differences in the BH2 sequence of the VP5 protein, infectivity and the VP2 sequence are not associated with the modulation of apoptosis.


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  1. Roberts R, Pearson M. Infectious Pancreatic Necrosis in Atlantic salmon, Salmo salar L. J Fish Dis 2005; 28:383-389. http://dx.doi.org/10.1111/j.1365-2761.2005.00642.x
  2. Bruslind L, Reno P. Virulence Comparison of Three Buhl-Subtipe Isolates of Infectious Pancreatic Necrosis Virus in Brook Trout Fry. JAAH 2000; 12:301-315.
  3. Shivappa R, Song H, Yao K, Aas-Eng A, Evensen Ø, Vakharia V. Molecular characterization of Sp serotype strains of infectious pancreatic necrosis virus exhibiting differences in virulence. Dis Aquat Org 2004; 61:23-32. http://dx.doi.org/10.3354/dao061023
  4. Song H, Santi N, Evensen Ø, Vakharia V. Molecular Determinants of Infectious Pancreatic Necrosis Virus Virulence and Cell Culture Adaptation. J Virol 2005; 79:10289-10299. http://dx.doi.org/10.1128/JVI.79.16.10289-10299.2005
  5. Hong J, Hsu Y, Wu J. Infectious pancreatic necrosis virus induces apoptósis due to down-regulation of survival factor MCL-1 protein expression in a fish cell line. Virus Res 1999; 63:75-83. http://dx.doi.org/10.1016/S0168-1702(99)00060-X
  6. Santi N, Song H, Vakharia V, Evensen Ø. Infectious Pancreatic Necrosis Virus VP5 Is Dispensable for Virulence and Persistence. J Virol 2005; 79:9206-9216. http://dx.doi.org/10.1128/JVI.79.14.9206-9216.2005
  7. Sano M, Okamoto H, Fukuda H, Saneyoshi M, Sano T. Virulence of infectious pancreatic necrosis virus is associated with the larger RNA segment (RNA segment A). J Fish Dis 1992; 15:283-293. http://dx.doi.org/10.1111/j.1365-2761.1992.tb00666.x
  8. Hong J, Gong H, Wu J. IPNV VP5, a Novel Anti-apoptósis gene of the Bcl-2 Family, Regulates Mcl-1 and Viral Protein Expression. Virol 2002; 295:217-229. http://dx.doi.org/10.1006/viro.2001.1336
  9. Weber S, Fichtner D, Mettenleiter T, Mundt E. Expression of VP5 of infectious pancreatic necrosis virus strain VR299 is initiated at the second in-frame start codon. J Gen Virol 2001; 82:805-812. http://dx.doi.org/10.1099/0022-1317-82-4-805
  10. Santi N, Sandtrø A, Sindre H, Song H, Hong J, Thu B, Wu J, Vakharia V, Evensen Ø. Infectious Pancreatic Necrosis Virus induces apoptósis In vitro and in vivo independent of VP5 expression. Virol 2005; 342:13-25. http://dx.doi.org/10.1016/j.virol.2005.07.028
  11. Hong J, Lin T, Hsu Y, Wu J. Apoptósis Precedes Necrosis of Fish Cell Line with Infectious Pancreatic Necrosis Virus Infection. Virol 1998; 250:76-84. http://dx.doi.org/10.1006/viro.1998.9347
  12. Galloux M, Libersou S, Morellet N, Bouaziz S, Da Costa B, Ouldali M, Lepault J, Delmas B. Infectious Bursal Disease Virus, a Non-enveloped Virus, Possesses a Capsid-associated peptide That Deforms and Perforates Biological Membranes. J Biol Chem 2007; 282:20774-20784. http://dx.doi.org/10.1074/jbc.M701048200
  13. Espinoza J, Cortés M, Kuznar J. Necrosis of infectious pancreatic necrosis virus (IPNV) infected cells rarely is preceded by apoptósis. Virus Res 2005; 109:133-138. http://dx.doi.org/10.1016/j.virusres.2004.10.014
  14. Imajoh M, Hirayama T, Oshima S. Frequent occurrence of apoptósis is not associated with pathogenic Infectious Pancreatic Necrosis Virus (IPNV) during persistent infection. Fish Shellfish Immunol 2005; 18:163-177. http://dx.doi.org/10.1016/j.fsi.2004.07.002
  15. O'Brien V. Viruses and Apoptósis. J Gen Virol 1998; 79:1833-1845. http://dx.doi.org/10.1099/0022-1317-79-8-1833
  16. Ortega C, Rodríguez S, de las Heras A, Romero A, Monrás M, Enríquez R. Evaluation of the level of Mx3 protein synthesis induced by infectious pancreatic necrosis virus (IPNV) strains of different infectivity. Vet Immunol Immunopathol 2011; 141:190–200. http://dx.doi.org/10.1016/j.vetimm.2011.02.022
  17. Reed J, Muench H. A simple method for estimating fifty percent end points. Am J Hyg 1938; 27:493-497.
  18. Cuconati A, White E. Viral homologs of BCL-2: role of apoptósis in the regulation of virus infection. Genes & Dev 2002; 16:465-2478. http://dx.doi.org/10.1101/gad.1012702
  19. Liu M, Vakharia V. Nonstructural Protein of Infectious Bursal Disease Virus Inhibits Apoptósis at the Early Stage of Virus Infection. J Virol 2006; 80:3369-3377. http://dx.doi.org/10.1128/JVI.80.7.3369-3377.2006
  20. Lombardo E, Maraver A, Espinosa I, Fernández-Arias A, Rodríguez J. VP5, the Nonstructural Polypeptide of Infectious Bursal Disease Virus, Accumulates within the Host Plasma Membrane and Induces Cell Lysis. Virol 2000; 277:345-357. http://dx.doi.org/10.1006/viro.2000.0595
  21. Brandt M, Yao K, Liu M, Heckert R, Vakharia V. Molecular determinants of virulence, cell tropism, and pathogenic phenotype of infectious bursal disease virus. J Virol 2001; 75:11974-11982. http://dx.doi.org/10.1128/JVI.75.24.11974-11982.2001
  22. Wolf K. Fish viruses and fish viral diseases. Ithaca, N.Y. Canstock Publishing Associates-Cornell University Press, 1988.
  23. Lyles D. Cytopathogenesis and Inhibition of Host Gene Expression by RNA Viruses. Microbiol Mol Biol Rev 2000; 64:709-724. http://dx.doi.org/10.1128/MMBR.64.4.709-724.2000
  24. Robertsen B. The interferon system of teleost fish. Fish Shellfish Immunol 2006; 20:172-191. http://dx.doi.org/10.1016/j.fsi.2005.01.010
  25. Chiu CL, Chou YL, Wu JL, Hong JR. Aquatic birnavirus capsid protein, VP3, induces apoptósis via the Bad-mediated mitochondria pathway in ï¬sh and mouse cells. Apoptósis 2010; 15:653-668. http://dx.doi.org/10.1007/s10495-010-0468-x
  26. Eléouet J, Druesne N, Chilmonczyk S, Momge D, Dorson M, Delmas B. Comparative Study of In-situ Cell Death Inducid by the Viruses of Viral Haemorrhagic Septicaemia (VHS) and Infectious Pancreatic Necrosis (IPN) in rainbow trout. J Comp Pathol 2001; 124:300-307. http://dx.doi.org/10.1053/jcpa.2001.0467
  27. Hong J, Wu J. Induction of apoptotic death in cells via Bad gene expression by Infectious pancreatic necrosis virus infection. Cell. Death Differ 2002; 9:113-124. http://dx.doi.org/10.1038/sj.cdd.4400933
  28. Joseph T, Cepica A, Brown L, Ikede B, Kibenge F. Mechanism of cell death during infectious salmon anemia virus infection is cell type-specific. J Gen Virol 2004; 85:3027-3036. http://dx.doi.org/10.1099/vir.0.80091-0

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