Nutrient absorption in lambs fed diets containing different amounts of phosphorus

Nutrient absorption in lambs fed diets containing different amounts of phosphorus

ABSTRACT

Objective. Evaluate the effect of increasing P intake on nutrient digestibility, and compare the true and apparent absorption coefficients of P and Ca in lambs. Materials and methods. Twenty-four Santa Ines sheep, with an average weight of 33.6 ± 1.6 kg, were distributed into four treatments (0, 2, 4 and 6 g/day of supplementary P) with forage: concentrate ratio of 70:30. The study of apparent nutrient digestibility was conducted during the first week, using the total feces collection method. During the second week, after injection of 7.4 MBq of 32P and 7.7 MBq of 45Ca, apparent (AAC) and true (TAC) absorption coefficients of P and Ca were determined. The lambs were kept in metabolic cages. Results. The increase in P intake did not affect (p>0.05) dry matter, crude protein, NDF or ADF digestibility, but the TAC of P and Ca and mineral matter digestibility decreased. The AAC was not affected (p=0.10). A cubic relationship was observed between P intake and TAC (TAC=2.16–1.95X+0.55X2-0.04X3; R2=0.38) and linear relationship with the TAC of Ca (TAC=0.559–0.03X; R2=0.26). TAC and AAC values were different (p<0.001). Conclusions. The increase in P intake doesn’t impact organic matter digestibility, but does affect P and Ca absorption. Apparent digestibility is not a reliable parameter to determine the efficiency of P and Ca absorption.

1. Borges ES, da Silva Filho JC, Roque NC, Olalquiaga JP, Vitti DMSS, Pati-o RM. Dinâmica do fósforo em ovinos alimentados com dietas contendo diversos níveis deste mineral. R Bras Zootec 2008; 37:1679-1684.
2. http://dx.doi.org/10.1590/S1516-35982008000900022
4. Owens FN, Sapienza DA, Hassen AT. Effect of nutrient composition of feeds on digestibility of organic matter by cattle: A review. J Anim Sci 2010; 88 Supl 1:E151-E169.
5. http://dx.doi.org/10.2527/jas.2009-2559
7. Bravo D, Sauvant D, Bogaert C, Meschy F. Quantitative aspects of phosphorus absorption in ruminants, part. II. Rep Nut Dev 2003; 43:271-284.
8. http://dx.doi.org/10.1051/rnd:2003020
10. Block HC, Erickson GE, Klopfenstein TJ. Review: re-evaluation of phosphorus requirements and phosphorus retention of feedlot cattle. Prof Anim Sci 2004; 20:319-329.
11. http://dx.doi.org/10.15232/S1080-7446(15)31321-8
13. Dove H, Charmley E. Relationships between phosphorus intake, plasma phosphorus and fecal and urinary phosphorus excretion in young sheep. Anim Prod 2004; 25:37-40.
15. National Research Council – NRC. Nutrient requirements of small ruminants: sheep, goats, cervids, and new word camelids. Washington D.C: National Academic Press; 2007. 7. Cappelle ER, Valadares Filho SC, Silva JFC, Cecon PR. Estimativas do valor energético a partir de características químicas e bromatológicas dos alimentos. Rev Bras Zoot 2001; 30:1837-1856. 8. Silva DJ, Queiroz AC. Análise de alimentos: Métodos químicos e biológicos. 3ra ed. Viçosa, Brasil: Editora UFV; 2002.
17. Campos FP de, Nussio CMB, Nussio LG. Métodos de análise de alimentos. 1ra ed. Piracicaba, Brasil: FEALQ; 2004.
19. Sarruge JR, Haag HP. Análises químicas em plantas. Piracicaba, Brasil: ESALQ/USP; 1974.
21. Zagatto EAG, Krug FJ, Bergamin Filho H. Jorgenson SS, Reis Bf. Merging zones in flow injection analysis. Part 2. Determination of calcium, magnesium and potassium in plant material by flow injection atomic absorption and flame emission spectrometry. Anal Chim Acta 1979; 104:279-284.
22. http://dx.doi.org/10.1016/S0003-2670(01)84009-5
24. Fiske CH, Subarrow Y. The colorimetric determination of phosphorus. J Biol Chem 1925; 66:375-400.
26. Nascimento Filho VF. Utilização do efeito Cerenkov na detecção de radionuclideos emissores de radiações beta. Piracicaba: Centro de Energia Nuclear na Agricultura; 1977.
28. Portilho FP, Vitti DMSS, Abdalla AL, McManus CM, Rezende MJM, Louvandini H. Minimum phosphorus requirement for Santa Inês lambs reared under tropical conditions. Small Rum Res 2006; 63:170-176.
29. http://dx.doi.org/10.1016/j.smallrumres.2005.03.006
31. Yan Q, Tang S, Bamikole NA, Han X, Zhou C, Wan M, Sun Z, Tan Z. Influence of dietary phosphorus variation on nutrient digestion, fecal endogenous phosphorus output and plasma parameters of goats. Livestock Sci 2011; 142:63-69.
32. http://dx.doi.org/10.1016/j.livsci.2011.06.019
34. Coneglian CM, Branco AF, Guimarães KC, Mano DS, Barreto JC, Fávaro BR. Replacement of dicalcium phosphate by rock phosphate in cattle diets: nutrient digestibility, plasma parameters, ruminal fermentation and microbial synthesis efficiency. R Bras Zootec 2010; 39:815-823.
35. http://dx.doi.org/10.1590/S1516-35982010000400017
37. Williams KB, Luca HF. Characterization of intestinal phosphate absorption using a model in vivo method. Am J Physiol Endocrinol Metab 2007; 292:E1917-E1921.
38. http://dx.doi.org/10.1152/ajpendo.00654.2006
40. Muscher A, Hattendorf J, Pfeffer E, Breves G, Huber K. Hormonal regulation of phosphate homeostasis in goats during transition to rumination. J Comp Physiol B Biochem, Syst Environ Physiol 2008; 178:585-596.
41. http://dx.doi.org/10.1007/s00360-007-0248-2
43. Commonwealth Scientific and Industrial Research Organization – CSIRO. Nutrient requirements of domesticated ruminants. Collingwood, Australia: CSIRO Publishing; 2007.
45. Underwood EJ, Suttle NF. Los minerales en la nutrición del ganado. Zaragoza, Espa-a: Editorial Acribia; 2003.
47. Pfeffer E, Beede DK, Valk H. Phosphorus metabolism in ruminants and requirements of cattle. In: Pfeffer E, Hristov A, editors. Nitrogen and phosphorus nutrition of cattle: reducing the environmental impact of cattle operations. London: CABI Publishing; 2005.
48. http://dx.doi.org/10.1079/9780851990132.0000
49. http://dx.doi.org/10.1079/9780851990132.0195
51. Roque AP, Dias RS, Vitti DMSS, Bueno IC, Da Cunha EA, Santos LE, Bueno MS. True digestibility of calcium from sources used in finishing Lamb diets. Small Rum Res 2007; 71:243-249.
52. http://dx.doi.org/10.1016/j.smallrumres.2006.07.004
54. Dias RS, Kebreab E, Vitti DMSS, Roque AP, Bueno ICS, France J. A revised model for studying phosphorus and calcium kinetics in growing sheep. J Anim Sci 2006; 84:2787-2794.
55. http://dx.doi.org/10.2527/jas.2006-082
57. Knowlton KF, Beede DK, Kebreab E. Phosphorus and calcium requirements of ruminants. In: Vitti DMSS, Kebreab E, editors. Phosphorus and calcium utilization and requirements in farms animals. London: CABI Publishing; 2010.
58. http://dx.doi.org/10.1079/9781845936266.0112
60. Dias RS, Kebreab E, Vitti DMSS, Portilho FP, Louvanidini H, France J. Phosphorus kinetics in lambs fed different levels of dicalcium phosphate. J Agric Sci 2007;145:509-516.
61. http://dx.doi.org/10.1017/S0021859607007150
63. Vittti DMSS, da Silva Flilho JC. Isotope dilution technique. In: Vitti DMSS, Kebreab E, editors. Phosphorus and calcium utilization and requirements in farms animals. London: CABI Publishing; 2010.
64. http://dx.doi.org/10.1079/9781845936266.0000
65. http://dx.doi.org/10.1079/9781845936266.0008
67. Buendía G, Mendoza G, Pinos-Rodríguez JM, González-Mu-oz S, Aranda E, Miranda L, Melgoza L. Influence of supplemental phytase on growth performance, digestión and phosphorus balance of lambs fed sorghum-based diets. Ital J Anim Sci 2010; 9:186-189