Efecto del ácido linoléico sobre la producción de las prostaglandinas PGF2α y PGE2 en células endometriales

Efecto del ácido linoléico sobre la producción de las prostaglandinas PGF2α y PGE2 en células endometriales

RESUMEN

Objetivo. Evaluar el efecto in vitro del ácido linoléico sobre la producción de PGF2α y PGE2 en células endometriales epiteliales bovinas (CEEP). Materiales y métodos. Se cultivaron CEEP aisladas de tejido uterino y se suplementaron con AL a diferentes concentraciones (1 μM, 10 μM, 100 μM), oxitocina (OT) (0.1 μM) e interferón trofoectodérmico bovino (bINT-τ) (50 ng/ml). Se cuantificó la PGF2α y PGE2 a distintos tiempos (12, 24 y 36h). En el control, la PGF2α en el sobrenadante aumentó en el tiempo de cultivo y fue 1.2 veces mayor que la producción de PGE2. Resultados. El ácido linoléico disminuyó la concentración de PGF2α (p<0.05) en el sobrenadante, y no afectó (p>0.05) la producción de PGE2. El efecto conjunto de AL y OT sobre la producción de PGF2α difirió para cada uno de los tiempos; el ácido linoléico inhibió parcialmente el efecto estimulante de la OT sobre la producción de PGE2, el efecto conjunto del AL y el bINT-τ aumentó (p<0.05) esta inhibición hasta la hora 24. Conclusiones. El ácido linoléico afecta negativamente la concentración de PGF2α en el sobrenadante a través del tiempo. Respecto a la PGE2 se concluye que el ácido linoléico por sí solo no afecta la concentración en el sobrenadante.

1. Parent J, Villeneuve C, Fortier MA. Evaluation of the contribution of cyclooxygenase 1 and cyclooxygenase 2 to the production of PGE2 and PGF2 alpha in epithelial cells from bovine endometrium. Reprod 2003; 4:539-47. http://dx.doi.org/10.1530/rep.0.1260539
3. Arosh JA, Banu SK, Chapdelaine P, Emond V, Kim JJ, MacLaren LA, Fortier MA. Molecular cloning and characterization of bovine prostaglandin E2 receptors EP2 and EP4: expression and regulation in endometrium and myometrium during the estrous cycle and early pregnancy. Endocrinol 2003; 7:3076-91. http://dx.doi.org/10.1210/en.2002-0088
5. Arosh JA, Banu SK, Kimmins S, Chapdelaine P, Maclaren LA, Fortier MA. Effect of interferontau on
7. prostaglandin biosynthesis, transport, and signaling at the time of maternal recognition of pregnancy in cattle: evidence of polycrine actions of prostaglandin E2. Endocrinol 2004; 11:5280-93.
9. Ta p i e r o H , C o u v r e u r P, Te w K D . Polyunsa tura ted fa t ty acids (PUFA) and eicosanoids in human health and pathologies. Biomed Pharmacother 2002; 56:215-222 http://dx.doi.org/10.1016/S0753-3322(02)00193-2
11. Gabler C, Drillich M, Fischer C, Holder C, Heuwieser W, Einspanier R. Endometrial expression of selected transcripts involved in prostaglandin synthesis in cows with endometritis. Theriogenol 2009; 6:993-1004. http://dx.doi.org/10.1016/j.theriogenology.2008.11.009
13. Salli U, Saito N, Stormshak F. Spatiotemporal interactions of myristoylated alanine-rich C kinase substrate (MARCKS) protein with the actin cytoskeleton and exocytosis of oxytocin upon prostaglandin F2alpha stimulation of bovine luteal cells. Biol Reprod 2003; 6:2053-8. http://dx.doi.org/10.1095/biolreprod.103.017640
15. Kim S, Choi Y, Spencer TE, Bazer FW. Effects of the estrous cycle, pregnancy and interferon tau on expression of cyclooxygenase two (COX-2) in ovine endometrium. Reprod Biol Endocrinol 2003; 1:58. http://dx.doi.org/10.1186/1477-7827-1-58
17. Acosta TJ, Bah MM, Korzekwa A, Woclawek I, Markiewicz W, Jaroczewski JJ et al. Acute changes in circulating concentrations of progesterone and nitric oxide and partial pressure of oxygen during prostaglandin f2alpha-induced luteolysis in cattle. J Reprod Dev 2008; 2:149-55.
19. Olivera M, Tarazona A, Ruíz T, Giraldo C. Modelo de luteólisis bovina. Rev Col Cienc Pec 2007; 20:387-393.
21. Mattos R, Guzeloglu A, Badinga L, Staples CR, Thatcher WW. Polyunsa tura ted fatty acids and bovine interferon-tau modify phorbol ester-induced secretion of prostaglandin F2 alpha and expression of prostaglandin endoperoxide synthase-2 and phospholipase-A2 in bovine endometrial cells. Biol Reprod 2003; 3:780-7 http://dx.doi.org/10.1095/biolreprod.102.015057
23. Herath S, Lilly ST, Fischer DP, Williams EJ, Dobson H, Bryant CE et al. Bacterial lipopolysaccharide induces an endocrine switch from prostaglandin F2alpha to prostaglandin E2 in bovine endometrium. Endocrinol 2009; 4:1912-20. http://dx.doi.org/10.1210/en.2008-1379
25. Hirata M, Sato T, Tsumagari M, Shimada A, Nakano H, Hashizume K et al. Differential regulation of the expression of matrix metalloproteinases and tissue inhibitors of metalloproteinases by cytokines and growth factors in bovine endometrial stromal cells and trophoblast cell line BT-1 in vitro. Biol Reprod 2003; 4:1276-81.
27. Tithof PK, Roberts MP, Guan W, Elgayyar M, Godkin JD. Distinct phospholipase A2 enzymes regulate prostaglandin E2 and F2alpha production by bovine endometrial epithelial cells. Reprod Biol Endocrinol 2007; 5:16. http://dx.doi.org/10.1186/1477-7827-5-16
29. Hermenegildo C, Oviedo PJ, Cano A. Cyclooxygenases regulation by estradiol on endothelium. Curr Pharm Des 2006; 2:205-15. http://dx.doi.org/10.2174/138161206775193136
31. Okuda K, Kasahara Y, Murakami S, Takahashi H, Woclawek I, Skarzynski DJ. Interferontau blocks the stimulatory effect of tumor necrosis factor-alpha on prostaglandin F2alpha synthesis by bovine endometrial stromal cells. Biol Reprod 2004; 1:191-7.
33. Cheng Z, Abayasekara DR, Wathes DC. The effect of supplementation with n-6 polyunsaturated fatty acids on 1-, 2- and 3-series prostaglandin F production by ovine uterine epithelial cells. Biochim Biophys Acta 2005; 2:128-35. http://dx.doi.org/10.1016/j.bbalip.2005.08.007
35. Caldari C, Rodriguez C, Greene ES, Badinga L. Differential effects of n-3 and n-6 fatty acids on prostaglandin F2alpha production by bovine endometrial cells. J Dairy Sci 2006; 3:971-7. http://dx.doi.org/10.3168/jds.S0022-0302(06)72162-2
37. Murakami S, Shibaya M, Takeuchi K, Skarzynski DJ, Okuda K. A passage and storage system for isolated bovine endometrial epithelial and stromal cells. J Reprod Dev 2003; 6:531-8. http://dx.doi.org/10.1262/jrd.49.531
39. Skarzynski D, Piotrowska K, Bah M, Korzekwa A, Woclawek I, Sawai K et al. Effects of exogenous tumour necrosis factor-alpha on the secretory function of the bovine reproductive tract depend on tumour necrosis factor-alpha concentrations. Reprod Domest Anim 2008; 9999:1-9.
41. Lenis Y, Olivera M, Tarazona A. Aislamiento, y caracterización de células epiteliales endometriales bovinas en cultivo primario. Rev Colomb Cienc Pecu 2009; 22-3: 566-80.
43. Meier S, Ledgard AM, Sato TA, Peterson AJ, Mitchell MD. Polyunsaturated fatty acids differentially alter PGF(2alpha) and PGE(2) release from bovine trophoblast and endometrial tissues during short-term culture. Anim Reprod Sci 2009; 2-4:353-60.
45. Mann GE. Hormone control of prostaglandin F(2 alpha) production and oxytocin receptor concentrations in bovine endometrium in explant culture. Domest Anim Endocrinol 2001; 3:217-26. http://dx.doi.org/10.1016/S0739-7240(01)00091-1
47. Wathes DC, Abayasekara DR, Aitken RJ. Polyunsaturated fatty acids in male and female reproduction. Biol Reprod 2007; 2:190-201. http://dx.doi.org/10.1095/biolreprod.107.060558
49. Kelley DS. Modulation of human immune and inflammatory responses by dietary fatty acids. Nutrition 2001; 7-8:669-73.
51. Weem s W, Weem s YS , Randel RD. Prostaglandins and reproduction in female farm animals. Vet J 2006; 171: 206–228. http://dx.doi.org/10.1016/j.tvjl.2004.11.014
53. Rodríguez C, Caldari C, Greene ES, Badinga L. Conjugated linoleic acid reduces phorbol ester-induced prostaglandin F2alpha production by bovine endometrial cells. J Dairy Sci 2006; 10:3826-32. http://dx.doi.org/10.3168/jds.S0022-0302(06)72424-9
55. Woclawek I, Acosta TJ, Korzekwa A, Bah MM, Shibaya M, Okuda K et al. Phytoestrogens modulate prostaglandin production in bovine endometrium: cell type specificity and intracellular mechanisms. Exp Biol Med (Maywood) 2005; 5:326-33.
57. Kotani Y, Iwase A, Ando H, Mizutani S. Oxytocin-induced prostaglandin E2 (PGE2) synthesis is regulated by progesterone via oxytocinase in Ishikawa cells. Horm Metab Res 2005; 37:1-9. http://dx.doi.org/10.1055/s-2005-861022
59. Farina MG, Billi S, Leguizamón G, Weissmann C, Guadagnoli T, Ribeiro ML et al. Secretory and cytosolic phospholipase A2 activities and expression are regulated by oxytocin and estradiol during labor. Reproduction 2007; 2:355-64. http://dx.doi.org/10.1530/REP-07-0078
61. Roberts MP. Interferon-tau, a Type 1 interferon envolved in maternal recognition of pregnancy. Cytokine Growth Factor Rev 2007; 18(5-6):403-408.