Prevalencia de la criptosporidiosis en seres humanos y terneros, y detección molecular del Cryptosporidium parvum

Prevalence of cryptosporidiosis in humans and calves and molecular detection of Cryptosporidium parvum

Objetivo. El objetivo de este estudio fue investigar la prevalencia de especies de Cryptosporidium en humanos y terneros en la provincia de Van, Turquía. Materiales y métodos. Se incluyeron en el estudio un total de 150 pacientes, incluidos 50 pacientes en hemodiálisis, 40 pacientes inmunosuprimidos con diarrea, 30 pacientes con diarrea solamente y 30 pacientes inmunocompetentes. Se recolectaron muestras de heces rectales de un total de 50 terneros alojados en establos y granjas en 10 aldeas centrales de Van, Turquía. Resultados. Se detectó Cryptosporidium parvum en el 17.3% de las 150 muestras de heces tomadas de seres humanos. C. parvum se observó en el 20% de los 50 pacientes en hemodiálisis, el 32.5% de los 40 pacientes inmunosuprimidos con diarrea y el 10% de los 30 pacientes con diarrea solamente, mientras que no hubo Cryptosporidium spp. detectado en los pacientes inmunocompetentes. C. parvum se observó en sólo el 6% de los 30 terneros diarreicos. Conclusiones. Claramente se entendio que la Criptosporidiosis fue detectada en una alta tasa en las muestras de los pacientes inmunosuprimidos sin y con sintomas de diarrea, y que además la especie activa que causó la enfermedad fue el agente etiologico Criptosporidium parvum. Por lo tanto, estos dos grupos de pacientes deben ser evaluados en lo que a términos de Criptosporidiosis se refiere.

1. Fayer R, Xiao L. Cryptosporidium and Cryptosporidiosis. 2nd ed. London: CRC press; 2008.
2. Laurent F, Lacroix-Lamandé S. Innate immune responses play a key role in controlling infection of the intestinal epithelium by Cryptosporidium. Int J Parasitol. 2017; 47:711–721. https://doi.org/10.1016/j.ijpara.2017.08.001
3. Xiao L. Molecular epidemiology of cryptosporidiosis: an update. Experimental Parasitology. 2010; 124:80-89. https://doi.org/10.1016/j.exppara.2009.03.018
4. Ryan U, Fayer R, Xiao L. Cryptosporidium species in humans and animals: current understanding and research needs. Parasitology. 2014; 141:1667-1685. https://doi.org/10.1017/S0031182014001085
5. Ryan U, Paparini A, Oskam CC. New technologies for detection of enteric parasites. Trends Parasitol. 2017; 33(7):532–546. https://doi.org/10.1016/j.pt.2017.03.005
6. Mirhashemi ME, Zintl A, Grant T, Lucy FE, Mulcahy G, De Waal T. Comparison of diagnostic techniques for the detection of Cryptosporidium oocysts in animal samples. Experimental parasitology. 2015; 151:14-20. https://doi.org/10.1016/j.exppara.2015.01.018
7. Yilmaz H, Cengiz ZT, Çiçek M. Investigation of cryptosporidiosis by enzyme-linked immunosorbent assay and microscopoy in children with diarrhea. Saudi Medical Journal. 2008; 29(4):526. https://smj.org.sa/content/29/4/526
8. Ekici A, Ünlü A, Halidi AG, Aydemir S, Yilmaz H. Determınatıon Of The Occurrence Frequency Of Cryptosporıdıum Sp. In Chıldren Brought To The Hospıtal Wıth The Complaınt Of Dıarrhea Usıng Dıfferent Methods. Applıed Ecology And Envıronmental Research. 2021; 19(4):2905-2913. http://dx.doi.org/10.15666/aeer/1904_29052913
9. Körkoca H, Yasar G, Atas AD, Kurtoglu Mg, Ekici K, Berktas M. Prevalence of Cryptosporidium spp. in asymptomatic food workers. Turkish Journal of Parasitology. 2013; 37(4):241. https://doi:10.5152/tpd.2013.2981
10. Ciçek M, Körkoca H, Gül A. Investigation of Cryptosporidium sp. in workers of the Van municipality slaughterhouse and in slaughtered. Animals. 2008; 32(1):8-11. https://www.turkiyeparazitolderg.org/archives/archive-detail/article-preview/van-belediye-mezbahasnda-alan-iilerde-ve-kesimi-ya/22898
11. Ayan A, Kilinc OO, Yuksek N, Basbugan Y. Detectıon of Cryptosporıdıum spp. in calves through nested Pcr And Kınyoun’s Acıd-Fast Methods In Van, Turkey. IJEES. 2020; 10(2):271-276. https://doi.org/10.31407/ijees10.205
12. Korkmaz M, Ok Ü. Parazitolojide laboratuvar. Turkish Parasitology Association. İzmir, Turkey; 2011.
13. Sadek GS. Use of nested PCR-RFLP for genotyping of Cryptosporidium parasites isolated from calves and children suffering from diarrhea. Parasitol United J. 2014; 7(2):129–137. https://doi.org/10.4103/1687-7942.149568
14. Akdemir C. Investigation of cryptosporidiosis by serologic and microscopic method and examination of tap water samples for oocysts. Turkiye Parazitol Derg. 2013; 37:9–12. https://doi.org/10.5152/tpd.2013.03
15. Yousry Hawash LS, Dorgham A, Mohammed, S. Prevalence of Cryptosporidium-associated diarrhea in a high altitude-community of Saudi Arabia detected by conventional and molecular methods. Korean J Parasitol. 2014; 52(5):479–485. https://doi.org/10.3347/kjp.2014.52.5.479
16. Karaman Ü, Daldal N, Özer A, Engiyurt Ö, Ertürk Ö. Incidence of Cryptosporidium spp. in the human population of Malatya in Turkey. Acta Medica. 2015; 31:263–269. http://abakus.inonu.edu.tr/xmlui/handle/123456789/15700
17. Nassar S, Oyekale T, Oluremi A. Prevalence of Cryptosporidium infection and related risk factors in children in Awo and Iragberi, Nigeria. J Immunoassay Immunochem. 2017; 38(1):2–9. https://doi.org/10.1080/15321819.2016.1178652
18. Karli A, Metin A, Ergen S, Simsir H, Köse G. The incidence of Cryptosporidium infection in children with diarrhea. Çocuk Enfeksiyon Dergisi. 2013; 7(3):92–96. https://doi.org/10.5152/ced.2013.27
19. Saksirisampant W, Prownebon J, Saksirisampant P, Mungthin M, Siripatanapipong S, Leelayoova S. Intestinal parasitic infections: prevalences in HIV/AIDS patients in a Thai AIDS-care centre. Annals of Tropical Medicine & Parasitology. 2009; 103(7):573-81. https://10.9775/kvfd.2010.2140
20. Börekçı G, Otağ F, Emekdaş G. Mersin’de bir gecekondu mahallesinde yaşayan ailelerde Cryptosporidium prevalansı. Journal of Infection. 2005, 19(1):39-34. http://infeksiyon.dergisi.org/summary_en.php3?id=105