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Efecto de Debaryomyces hansenii en la respuesta antioxidante de juveniles de camarón blanco Litopenaeus vannamei

Efecto de Debaryomyces hansenii en la respuesta antioxidante de juveniles de camarón blanco Litopenaeus vannamei



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Cómo citar
Pacheco M, M., Campa C, Ángel, Aguirre G, G., Luna G, A., Guzmán M, M., & Ascencio, F. (2012). Efecto de Debaryomyces hansenii en la respuesta antioxidante de juveniles de camarón blanco Litopenaeus vannamei. Revista MVZ Córdoba, 17(1), 2820-2826. https://doi.org/10.21897/rmvz.249

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PlumX
María Pacheco M
Ángel Campa C
Gabriel Aguirre G
Antonio Luna G
María Guzmán M
Felipe Ascencio

RESUMEN

Objetivo. Determinar la respuesta antioxidante [actividad de superóxido dismutasa (SOD) y catalasa (CAT)] así como la cuenta total de hemocitos (CTH) y el contenido de proteínas (CP) en camarones (Litopenaeus vannamei) expuestos a diferentes dosis y cepas de la levadura Debaryomyces hansenii (DH5, DH6, LL1), y un inmunoestimulante comercial (LAM). Materiales y métodos. Las levaduras fueron cultivadas y suministradas diariamente en concentraciones diferentes (104 – 106 UFC/mL) directamente a los tanques de cultivo de los camarones (8 ± 0.2 g) mientras que LAM fue aplicado una vez a la semana (0.5 mg/L). Los organismos fueron mantenidos bajo condiciones de laboratorio (28°C, 35%, 80% de recambio diario de agua, dieta comercial para camarón ad libitum). Los tratamientos fueron distribuidos por duplicado y los resultados evaluados a los 15 días con un análisis de varianza y una prueba de Tukey. Resultados. Se registró un CTH significativo (p<0.05) en los tratamientos con DH6 y LL1 (106 UFC/mL) comparada con el control, mientras que las cepas DH5 y DH6 revelaron un incremento significativo (p<0.05) de CP con la dosis de 104 UFC/mL. Los camarones tratados con LAM incrementaron significativamente (p<0.05) los valores de SOD y CAT. Conclusiones. Los resultados obtenidos demuestran que D. hansenii incrementa la respuesta antioxidante y CTH en camarones.


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