Concentración sérica de la hormona anti-Mülleriana y su relación con la reserva ovárica en vacas Brahman donantes de ovocitos

Serum concentration of anti-Müllerian hormone and its relationship with ovarian reserve in Brahman oocyte donors

Objetivo. Evaluar la relación de la concentración sanguínea de AMH con el recuento folicular ovárico y la producción in vitro de embriones en hembras bovinas de la raza Brahman. Materiales y métodos. Para estandarizar la técnica de cuantificación de AMH se realizó un primer experimento, en el cual se tomaron muestras de sangre de 10 hembras Brahman sincronizadas en su celo, en tres días diferentes del ciclo estral, con más de 90 días posparto y con evaluación reproductiva normal. La concentración sérica de AMH se determinó con un kit inmunoenzimático comercial. Una vez estandarizada la técnica, se realizó un segundo experimento, se tomaron muestras de sangre de 100 donantes Brahman de ovocitos no sincronizadas, se realizó una sesión de aspiración folicular para la producción in vitro de embriones y se registró el número de folículos mayores de 2 mm en los dos ovarios. Resultados. No hubo diferencias en la concentración de AMH entre los días evaluados del ciclo estral y se encontró una correlación de 0,82 (p<0.001) entre la población de folículos antrales (PFA) y la concentración de AMH. La concentración sérica de AMH osciló entre 0.02 y 2.69 ng/ml. Además, se encontró una correlación de 0.73 (p<0.001) entre AMH y AFP y 0.54 entre AMH y el porcentaje de blastocistos producidos. Conclusiones. La AMH se puede utilizar como un marcador endocrino satisfactorio de la predicción de la reserva ovárica para la producción de embriones in vitro en ganado Brahman.

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