Effect of different levels of L-carnitine and lysine-methionine on broiler blood parameters


Babak Hosseintabar
Mohammad Dadashbeiki
Mehrdad Bouyeh
Alireza Seidavi
René van den Hoven
Sandra Gamboa


Objetive. In the present study a completely randomized 3×3 factorial design was used to analyze the effects of different levels of L-Carnitine, lysine(Lys) and methionine (Met) on the blood concentrations of energy, protein and lipid metabolites of male broiler chickens. Materials and methods. A total of 270 newly hatched male broiler chickens (Ross 308) were randomly assigned to 9 groups (ten broilers per replicate and three replicates per treatment). The control group was fed a basal diet, whereas the treatment groups were fed basal diets supplemented with L-Carnitine (0 mg/kg, 75 mg/kg and 150 mg/kg) and lysine-methionine (0, 15 and 30%) for 42 days. On day 42, one bird was randomly chosen per replication, a blood sample was taken and the blood concentrations of glucose (GLU), uric acid (UAc), triglyceride (TG), VLDL, HDL, LDL, total protein (TP), albumin (Alb) and total cholesterol (TC) were analyzed. Results. Dietary L-carnitine supplementation had a significant effect (p<0.05) on uric acid (UAc), HDL, LDL, and total cholesterol (TC). The birds feed L-carnitine plus Lys and Met presented the highest plasmatic UAc level and the lowest plasmatic TC and LDL level. Moreover, L-carnitine significantly reduced total cholesterol (TC) when compared with both the control group and the birds feed Lys and Met without L-carnitine. Conclusions. A diet with 150 mg/kg L-carnitine plus 15% Lys and Met seems to be enough to sustain low plasmatic TC, LDL and HDL concentrations on male broiler.


Objetivo. Se realizó un estudio para determinar el efecto de la suplementación deL-carnitina, lisina (Lys) y metionina (Met) sobre los metabolitos sanguíneos de pollos de engorde. Materiales y métodos. Se utilizaran 270 pollos de la línea Ross 308 de un día de edad y se dividieron en 9 tratamientos en un diseño al azar con arreglo factorial 3x3: tres niveles de L-carnitina (0 mg/kg, 75 mg/kg y 150 mg/kg) y tres de lisina-metionina (0, 15 y 30%) durante 42 días. Cada tratamiento constó de 3 repeticiones con 10 pollos por repetición. El día 42 de edad, se tomaron muestras de sangre de tres aves por tratamiento para cuantificar niveles séricos de glucosa (GLU), ácido úrico (UAc), triglicéridos (TG), VLDL, HDL, LDL, proteínas totales (PT), albúmina (Alb) y colesterol total (TC). Resultados. La suplementación de L-carnitina en la dieta tuvo un efecto significativo (p<0.05) en los niveles de ácido úrico en suero (UAC), HDL, LDL y colesterol total (CT). Las aves alimentadas con L-carnitina más Lys y Met mostraron niveles séricos más altos de UAc y menor TCy LDL. Por otra parte, la L-carnitina redujó significativamente el colesterol total (CT), cuando se comparó con el grupo control y con los pollos alimentados con Lys y Met, sin L-carnitina. Conclusiones. Una dieta con 150 mg/kg de L-carnitina y 15% Lys y Met parece ser suficiente para mantener bajas concentraciones plasmáticas de TC, LDL y HDL en pollos de engorde.

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