Concordance between otic cytology and culture in diagnosis of external otitis canine by Malassezia spp


Adriana Pulido-Villamarín
Rubiela Castañeda-Salazar
Melva Linares-Linares
Marcela Mercado-Reyes


Objective. To determine the correlation between microbiological culture and otic cytology for diagnoses of external otitis by Malassezia in dogs. Materials and methods. 158 ear swabs of dogs with clinical diagnosis of external otitis were analyzed by cytology, mycological culture and metabolic tests. Results. Were obtained a positive results by cytology of 62% and 75.3% by culture. The 31.1% of isolates were identified as M. pachydermatis, 12.6% as M. furfur and 56.3% were classified as Malassezia spp., because was not possible to define the species. We found a positive concordance between cytology and culture for Malassezia spp., of 0.76 with a kappa index of 0.448 (95% CI 0.30 to 0.60) which represents a moderate strength of concordance between the two techniques, without regard the identified species. Conclusions. The use of a diagnostic test is not enough to establish the participation of Malassezia spp., as a causal disease agent.

Palabras clave


Los datos de descargas todavía no están disponibles.


Referencias / Ver

Dragonetti AM, Broglia G. Otitis externa canina aproximación al diagnóstico. Vet Cuyana 2007; 1-2:28-33.

Zur G, Lifshitz B, Bdolah-Abram T. The association between the signalment, common causes of canine otitis externa and pathogens. J Small Anim Pract 2011; 52:254–258.

Girão MD, Prado MR, Brilhante RSN, Cordeiro RA, Monteiro AJ, Sidrim JJC and Rocha MFG. Malassezia pachydermatis isolated from normal and diseased external ear canals in dogs: A comparative analysis. The Vet J 2006; 172:544–548.

Khosravi AR, Eid S, Ziglari T, Bayat M. Isolation and Differentiation of Malassezia Species Isolated from Healthy and Affected Small Animals, Ear and Skin. World J Zool 2008; 3(2):77-80.

Sarierler M, Kirkan S. Microbiological Diagnosis and Therapy of Canine Otitis Externa. Veteriner Cerrahi Dergisi 2004; 10(3-4):11-15

Petrov V, Mihaylov G. Malassezia pachydermatis - etiology and clinical findings in canine external otitis – therapeutic approaches. Trakia J Sci (TJS) 2008; 6(1):123-126.

Brito EHS, Fontenelle ROS, Brilhante RSN, Cordeiro RA, Monteiro AJ, Sidrim JJC, Rocha MFG. The anatomical distribution and antimicrobial susceptibility of yeast species isolated from healthy dogs. Vet J 2009; 182:320–326.

Murphy KM. A review of techniques for the investigation of otitis externa and otitis media. Clin Tech Small Anim Pract 2001; 16(3):236-241.

Crespo MJ, Abarca ML, Caba-es FJ. Occurrence of Malassezia spp. in the external ear Canals of dogs and cats with and without otits externa. Med Mycol 2002; 40:115-121.

Rosser EJ. Causes of otitis externa. Vet Clin North Am Small Anim Pract 2004; 34:459-468.

Guého E, Midgley G, Guillot J. The genus Malassezia with description of four new species. Antonie van Leeuwenhoek 1996; 69:337–355.

Guillot J, Guého E, Lesourd M, Midgley G, Chévrier G, Dupont B. Identification of Malassezia species. J Mycol Med 1996; 6:103-110.

Sugita T, Takashima M, Shinoda T, Suto H, Unno T, Tsuboi R, Ogawa H, Nishikawa A. New yeast species Malassezia dermatis isolated from patients with atopic dermatitis. J Clinic Microbiol 2002; 41:4695-4699.

Hirai A, Kano R, Makimura E, Duarte R, Hamdam M, Lachance A, Yamaguchi A, Hasegawa A. Malassezia nana sp. nov., anovel lipid-dependent yeast species isolated from animals. Int J Syst Evol Microbiol 2004; 54:623-627.

Ashbee R, Bignell EM. Pathogenic yeast: The yeast handbook. Berlin: Springer; 2010.

Caba-es FJ, Vega S, Castellá G. Malassezia cuniculi sp. nov., a novel yeast species isolated from rabbit skin. Med Mycol 2011; 49:40-48.

Angus JC. Otic citology in health and disease. Vet Clin North Am Small Anim Pract 2004; 34:411-424.

Fernández G, Barboza G, Villalobos A, Parra O, Finol G, Ramírez RA. Isolation and identification of microorganisms present in 53 dogs suffering otitis externa. Rev Cient FCV-LUZ 2006; 16(1):23-30.

Nobre MO, Pötter De Castro A, Nascente PS, Ferreiro L, Meireles MCA. Occurrence of Malassezia pachydermatis and other infectious agents as cause of external otitis in dogs from Rio Grande do Sul state, Brazil (1996/1997). Braz J Mycrobiol 2001; 32:245-249.

Crespo MJ, Abarca ML, Caba-es FJ. Atypical lipid-dependent Malassezia species isolated from dogs with otitis externa. J Clin Microbiol 2000; 38(6):2383–2385.

Ginel PJ, Lucena R, Rodríguez JC, Ortega J. A semiquantitative cytological evlauation of normal and pathological samples from the ear canal of dogs and cats. Vet Dermatol 2002; 13:151-156.

Boehringer SI. Valor diagnóstico del examen citológico en las otitis externas de caninos. Rev Vet 2011; 22:38-42.

Citado por

Artículos más leídos del mismo autor/a