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AutoresFunda Özata Kerem Ural
Objectives. In the present study alterations in trombocyte numbers and trombocyte indices were investigated in 51 dogs naturally infected with E. canis and/or A. phagocytophilum. Achieved results were compared to those of 20 healty dogs comprising control group. Materials and methods. Naturally occuring vector borne diseases were diagnosed by use of a canine point-of-care ELISA kit (Snap 4Dx, Idexx). Dogs were enrolled into 3 groups as follows; II. group involved A. phagocytophilum infected dogs (n=10), III. group (n=13) E. canis+ A. phagocytophilum co-infected, and IV. group (n=28) E. canis infected dogs. Healthy controls (n=20) were enrolled in group I. Results. Mean PLT counts were significantly decreased in II., III. and IV. groups (159.6±63.5, 142.3±44.3, 148.7±33.5, respectively) in comparison to control group (370.4±28.6) (p≤0.01). Mean PCT values in groups II., III. and IV. (0.1530±0.590, 0.1531±0.0441, 0.1450±0.314, respectively) were significantly decreased in contrast to control group (0.3695±0.0283) (p≤0.01). Between PLT and PCT values, statistically significant positive correlation (p≤0.01) (r=0.988, 0.990 and 0.981, respectively) was evident among groups II., III. and IV. Conclusions. Infected dogs showed significant alterations (p≤0.01) among mean PLT and PCT values and a positive correlation was evident between those 2 parameters (p≤0.01), whereas alterations on mean MPV and PDWc were not statistically significant. Finally it was suggested that according to the aforimentioned results, PLT and PCT values may be used as valuable parameters for diagnosis and probably for monitorization and prognosis in infected dogs with Ehrlichiosis and/or Anaplasmosis
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2. Bulla C, Takahira RK, Araújo JP, Trinca AL, Lopes SR, Wiedmeyer CE. The relationship between the degree of thrombocytopenia and infection with Ehrlichia canis in an endemic area. Vet Res 2004; 35(1):141-146. http://dx.doi.org/10.1051/vetres:2003038
3. Bexfield NH, Villiers EJ, Herrtage ME. Immune-mediated haemolytic anaemia and thrombocytopenia associated with Anaplasma phagocytophilum in a dog. J Small Anim Pract 2005; 46:543-548. http://dx.doi.org/10.1111/j.1748-5827.2005.tb00284.x
4. Mazepa AW, Kidd LB, Young KM, Trepanier LA. Clinical presentation of 26 Anaplasma phagocytophilum seropositive dogs residing in an endemic area. J Am Anim Hosp Assoc 2010; 46(6):405-412. http://dx.doi.org/10.5326/0460405
5. Borjesson DL, Simon SI, Tablin F, Barthold SW. Thrombocytopenia in a mouse model of human granulocytic ehrlichiosis. J Infect Dis 2001; 184:1475-1479. http://dx.doi.org/10.1086/324518
6. Lester SJ, Breitschwerdt EB, Collis CD, Hegarty BC. Anaplasma phagocytophilum infection (granulocytic anaplasmosis) in a dog from Vancouver Island. Can Vet J 2005; 46:825-827.
7. Granick JL, Reneer DV, Carlyon JA. Anaplasma phagocytophilum infects cells of the megakaryocyte lineage through sialyated ligands but fails to alter platelet production. J Med Microbiol 2008; 57:416–423. http://dx.doi.org/10.1099/jmm.0.47551-0
8. Waner T. Hematological changes in dogs infected with Ehrlichia canis. Isr J Vet Med 2008; 63:1.
9. Nakaghi HCA, Machado ZR, Costa TM, Andre RM, Baldani DC. Canine ehrlichiosis: clinical, hamatological, serological and molecular aspect. Cien Rural 2008; 38(3):776-770. http://dx.doi.org/10.1590/S0103-84782008000300027
10. Castro MB, Machado RZ, de Aguino LP, Alessi AC, Costa MT. Exprimental acute canine monocytic ehrlichiosis: clinicopathological and immunopathological findings. Vet Pathol 2004; 119(1):73-86.
11. Boudreaux, M.K. & Ebbe, S. Comparison of platelet number, mean platelet volume and platelet mass in five mammalian species. Comp Clin Pathol 1998; 8:16-20. http://dx.doi.org/10.1007/bf02628099
12. Moritz, A., Walcheck, B.K. & Weiss, D.J. Evaluation of flow cytometric and automated methods for detection of activated platelets in dogs with inflammatory disease. Am J Vet Res 2005; 66:325-329. http://dx.doi.org/10.2460/ajvr.2005.66.325
13. Sullivan PS, Manning KL, Mcdonald TP. Association of mean platelet volume and bone marrow megakaryocytopoiesis in thrombocytopenic dogs: 60 cases (1984–1993). J Am Vet Med Assoc 1995; 206:332-334.
14. Rafaj RB, Mrljak V, Guelfi JF. Number of thrombocytes and mean platelet volume in canine babesiosis. Rev Med Vet 2005; 156:95-98.
15. Bath PMW, Butterworth RJ. Platelet size: measurement, physiology and vascular disease. Blood Coagul Fibrin 1996; 7:157-161. http://dx.doi.org/10.1097/00001721-199603000-00011
16. Yιlmaz Z, Eralp O, Ilcol Y. Evaluation of platelet count and its association with plateletcrit, mean platelet volume and platelet size distribution width in a canine model of endotoxemia. Vet Clin Pathol 2008; 159-163.
17. Zvorc Z, Rafaj R B, Mrljak V. Erythrocyte and platelet indices in babesiosis of dogs. Vet Arhiv 2010; 80(2):259-267.
18. Zygner W, Gojska O, Rapacka G, Jaros D, Wedrychowιcz H. Hematological changes during the course of canine babesiosis caused by large Babesia iin domestic dogs in Warsaw (Poland). Vet Parasitol; 2007; 145,146-15 http://dx.doi.org/10.1016/j.vetpar.2006.11.008
19. Wiwanitkit V. Plateletcrit, mean platelet volume, platelet distribution width: Its expected values and correlation with parallel red blood cell parameters. Clin Appl Thromb Hemost 2004; 10(2):175. 50.
20. Khandekar MM, Khuruana AS, Deshmukh SD, Kakrani AL, Katdare AD, Inamdar AK. Platelet volume indices in patients with coronary artery disease and acute myocardial infarction: an Indian scenario. J Clin Pathol 2006; 59:146-149. http://dx.doi.org/10.1136/jcp.2004.025387