Ir al menú de navegación principal Ir al contenido principal Ir al pie de página del sitio

Efecto gastroprotector del extracto de Hypericum perforatum sobre la úlcera gástrica inducida por indometacina en ratas

Gastroprotective effect of Hypericum perforatum extract on indomethacin induced gastric ulcer in rats



Cómo citar
Avcıoğlu, M. ., & Akar, F. (2022). Efecto gastroprotector del extracto de Hypericum perforatum sobre la úlcera gástrica inducida por indometacina en ratas. Revista MVZ Córdoba, 27(2), e2355. https://doi.org/10.21897/rmvz.2355

Dimensions
PlumX
Licencia
Creative Commons License

Esta obra está bajo una licencia internacional Creative Commons Atribución-NoComercial-CompartirIgual 4.0.

Merve Avcıoğlu
Ferda Akar

Objetivo. Hypericum perforatum L. (H. perforatum) es una hierba tradicional utilizada en el tratamiento de muchas enfermedades. El extracto de H. perforatum tiene efectos antimicrobianos, antioxidantes, antiinflamatorios, antiulcerosos y antidepresivos. La indometacina es un fármaco antiinflamatorio no esteroideo. Puede causar daño oxidativo en el tejido del estómago. Para este propósito, se investigó la eficacia protectora del extracto de H. perforatum en un modelo de úlcera gástrica inducida por indometacina. Materiales y métodos. Se utilizaron sesenta ratas macho albinas Wistar y las ratas se dividieron en 6 grupos como control negativo, control positivo, H. perforatum, extracto de H. perforatum+indometacina 10/25, extracto de H. perforatum+indometacina 25/25, extracto de H. perforatum+indometacina 50/25. Se administraron por vía oral diferentes dosis de extracto de H. perforatum por sonda, y después de 5 minutos se indujo la úlcera gástrica con indometacina (25 mg/kg). Después de 6 horas, se sacrificaron las ratas. Se midieron los índices de úlceras para cada estómago. Se analizaron los niveles de glutatión (GSH), catalasa (CAT), malondialdehído (MDA) y superóxido dismutasa (SOD) en el tejido del estómago. Resultados. El extracto de H. perforatum+indometacina 50/25 aumentó la actividad de SOD y el nivel de GSH y disminuyó los niveles de MDA en la úlcera gástrica inducida por indometacina. Sin embargo, el índice de úlcera fue significativamente menor solo en el grupo que recibió el extracto de H. perforatum. Conclusiones. Este estudio indica que el extracto de H. perforatum puede tener como potencial agente alternativo en el tratamiento de la úlcera gástrica inducida por indometacina.


Visitas del artículo 685 | Visitas PDF


Descargas

Los datos de descarga todavía no están disponibles.
  1. Guyton AC. Texbook of Medical Physiology. Physiology of Gastrointestinal Disorders. 11thed. Philadelphia: Saunders Company; 2006; 819-822. https://www.moscmm.org/pdf/Guyton%20physiology.pdf
  2. Kaya S. Veteriner Farmakoloji 5. Baskı Cilt 2, Kaya S, Pirinçci İ, Ünsal A, Traş B, Bilgili A, Akar F (eds) Medisan Yayınevi: Ankara; 2009.
  3. Kurumbail R, Kiefer JR, Marnett LJ. Cyclooxygenase enzymes: Catalysis and inhibition. Curr Opin Struct Biol. 2001; 11(6):752-760. https://doi.org/10.1016/s0959-440x(01)00277-9
  4. Suleyman H, Demircan B, Karagoz Y. Anti-inflammatory and side effects of cyclooxygenase inhibitors. Pharmacol Rep. 2007; 59:247-258. https://pubmed.ncbi.nlm.nih.gov/17652824/
  5. Baskose I, Savran A. A new species from southern Anatolia (Dedegöl Mountain Series — Çürük Mountain) in Turkey: Hypericum bilgehan-bilgilii (Hypericaceae). Phytotaxa. 2018; 374(2):110-118. https://doi.org/10.11646/phytotaxa.374.2.2
  6. Ersoy E, Özkan EE, Mat A. Pharmacological Actiivities of Hypericum Species in Light of New Studies. JARHS. 2019; 2(2):71-79. https://doi.org/10.26650/JARHS2019-616370
  7. Greeson J, Sanford B, Monti DA. St. John’s worth (Hypericum perforatum): a review of the current pharmacological, toxicological and clinical literature. Psychopharmacology 2001; 153:402-414. https://doi.org/10.1007/s002130000625
  8. Öztürk N, Korkmaz S, Öztürk Y. Wound-healing activity of St. John’s Wort (L.) on chicken embryonic fibroblasts. J Ethnopharmacol. 2007; 111:33-39. https://doi.org/10.1016/j.jep.2006.10.029
  9. Prisacaru AI, Andritoiu C, Andriescu C. Evaluation of the wound-healing effect of a novel Hypericum perforatum ointment in skin injury. Rom J Morphol Embryol. 2013; 54(4):1053-1059. https://pubmed.ncbi.nlm.nih.gov/24399001/
  10. Kıyan S, Uyanıkgil Y, Altuncı YA, Çavuşoğlu T, Uyanikgil EOC, Karabey F. Investigation of acute effects of Hypericum perforatum (St. John’s Wort-Kantaron) treatment in experimental thermal burns and comparison with silver sulfadiazine treatment, Ulus Travma Acil Cerrahi Derg. 2015; 21: 323-336. https://dx.doi.org/10.5505/tjtes.2015.63822
  11. Arsić I, Žugić A, Antić DR, Zdunić G, Dekanski D, Marković G, Tadić V. Hypericum Perforatum L. Hypericaceae/Guttiferae Sunflower, Olive and Palm Oil Еxtracts Attenuate Cold Restraint Stress –Induced Gastric Lesions. Molecules 2010; 15:6688-6698. https://doi.org/10.3390/molecules15106688
  12. Karaboğa İ, Dökmeci AH, Ovalı MA, Yılmaz A. Etanol Uyarımlı Sıçan Akut Mide Mukoza Hasar Modelinde Hypericum perforatum’un Koruyucu Etkilerinin İncelenmesi. NKMJ 2017; 5(3):99-108. https://dergipark.org.tr/tr/download/issue-full-file/33411
  13. Abdel-Salam OME. Anti-Inflammatory, Antinociceptive and Gastric Effects of Hypericum perforatum in Rats. ScientificWorldJournal. 2005; 5:585–596. https://doi.org/10.1100/tsw.2005.78
  14. Odabaşoğlu F, Halici Z, Cakir A, Halici M, Aygun H. Beneficial effects of vegetable oils (corn, olive and sunflower oils) and α-tocopherol on anti-inflammatory and gastrointestinal profiles of indomethacin rats. Eur. J. Pharmaco. 2008; 591:300-306. https://doi.org/10.1016/j.ejphar.2008.06.075
  15. Guidobono F, Pagani F, Ticozzi C, Sibilia V, Pecile A, Netti C. Protection by amylin of gastric erosions induced by indomethacin or ethanol in rats. Br J Clin Pharmacol 1997; 120(4): 581-6. https://doi.org/10.1038/sj.bjp.0700941
  16. Sun Y, Oberley LW, Li Y. A simple for clinical assay of superoxide dismutase. Clin Chem. 1988; 34:497-500. https://pubmed.ncbi.nlm.nih.gov/3349599/
  17. Aebi H. Catalase in vitro assay methods. Methods in Enzymology. 1984; 105:121-126. https://doi.org/10.1016/s0076-6879(84)05016-3
  18. Tietze F. Enzymic method for quantitative determination of nanogram amounts of total and oxidized glutathione: applications to mammalian blood and other tissues. Anal Biochem. 1969; 27(3):502-522. https://doi.org/10.1016/0003-2697(69)90064-5
  19. Ohkawa H, Ohishi N, Yagi K. Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Anal Biochem. 1979; 95:351-358. https://doi.org/10.1016/0003-2697(79)90738-3
  20. Nguelefack TB, Feumebo CB, Ateufack G, Watcho P, Tatsimo S, Atsamo D, Tane P, Kaman-yi A. Anti-ulcerogenic properties of the aqueous and methanol extracts from the leaves of Solanum torvum Swartz (Solanaceae) in rats. J Ethnopharmacol 2005; 119(1): 135-40. https://doi.org/10.1016/j.jep.2008.06.008
  21. Schubert ML. Gastric secretion. Curr Opin Gastroenterol. 2014; 30: 578-582. https://doi.org/10.1097/MOG.0000000000000125
  22. Kılıçarslan H, Kalyon S, Yenice N. Etiopathogenesis of Peptic Ulser, Okmeydanı Tıp Dergisi. 2011; 27(2):65-69. http://doi.org/10.5222/otd.2011.065
  23. Eminler AT, Uslan Mİ, Köksal AŞ, Parlak E. Non-Steroid Anti-İnflamatuvar İlaçların Üst Gastrointestinal Sistem Yan Etkileri ve Önlenmesi. Güncel Gastroenteroloji. 2014; 18(3):333-338. http://guncel.tgv.org.tr/journal/53/pdf/100245.pdf
  24. Dündar Y, Aslan R. Hekimlikte Oksidatif Stres, Afyon Kocatepe Üniversitesi Yayınları: Ankara; 2000.
  25. Zdunic G, Godevac D, Milenkovic M, Vucievic D, Savikin K, Menkovic N, et al. Evalution of Hypericum perforatum oil extract for an antiinflammatory and gastroprotective activity in rats. Phytotherapy Research. 2009; 23(11):1559-1564. https://doi.org/10.1002/ptr.2809
  26. Kurt H, Özbayer, C, Değirmenci İ, Burukoğlu D, Saadat SM, Üstüner MC, et al. İndomethazine Bağlı Oluşan Gastrik Mukozal Hasar Üzerine Hypericum Perforatum Yağının Koruyucu Etkisi. Bozok Tıp Derg. 2016; 6(3):46-52. https://app.trdizin.gov.tr/makale/TWpVM05qQTFOUT09/indomethazine-bagli-olusan-gastrik-mukozal-hasar-uzerine-hypericum
  27. Cayci MK, Dayioglu H. Hypericum perforatum extracts healed gastric lesions induced by hypothermic restraint stress in Wistar rats. Saudi Med J. 2009; 30(6):750-754. https://pubmed.ncbi.nlm.nih.gov/19526154/
  28. Vieira SM, Silva RL, Lemos HP, Amorim RC, Silva EC, Reinach PS, Cunha FQ, Pohlit AM, Cunha TM. Gastro-protective effects of isobrucein B, a quassinoid isolated from Picrolemma sprucei. Fitoterapia. 2014; 95:8-15. https://doi.org/10.1016/j.fitote.2014.02.008
  29. Diniz PB, Ribeiro AR, Estevam CS, Bani CC, Thomazzi SM. Possible mechanisms of action of Caesalpinia pyramidalis against ethanol-induced gastric damage. J Ethnopharmacol. 2015; 20(168):79-86. https://doi.org/10.1016/j.jep.2015.03.054
  30. Yoshiwaka T, Naito Y, Kishi A, Tomii T, Kaneko T, Iinuma S, et al. Role of active oxygen, lipid peroxidation and antioxidants in the pathogenesis of gastric mucosal injury induced by indomethacin in rats. Gut. 1993; 34(6):732-737. https://doi.org/10.1136/gut.34.6.732
  31. Dengiz GÖ, Gürsan N. Effects of Momordica charantia L. (Cucurbitaceae) on indomethacin-induced ulcer models in rats. Turk J Gastroenterol. 2005; 16(2):85-88. https://pubmed.ncbi.nlm.nih.gov/16252198/
  32. Kim JH, Kim BW, Kwon HJ, Nam SW. Curative Effect of Selenium Against Indomethacin- Induced Gastric Ulcers in Rats. Microbial Biotechnology. 2011; 21(4):400-404. https://pubmed.ncbi.nlm.nih.gov/21532324/
  33. El-Masry TA, Elahwel AM, Emara AM. Study on treating ethanol-induced gastric lesions with omeprazole, Nigella sativa oil or both. Toxicological and Enviromental Chemistry. 2010; 92:1765-1782. https://doi.org/10.1080/02772241003730589
  34. Boyacioglu M, Kum C, Sekkin S, Yalinkilinc HS, Avci H, Epikmen ET, Karademir U. The effects of lycopene on DNA damage and oxidative stress on indomethacin-induced gastric ulcer in rats. Clin Nutr. 2016; 35:428-435. https://doi.org/10.1016/j.clnu.2015.03.006
  35. Chattopadhyay I, Bandyopadhyay U, Biswas K, Maity P, Banerjee RK. Indomethacin inactivates gastric peroxidase to induce reactive-oxygen-mediated gastric mucosal injury and curcumin protects it by preventing peroxidase inactivation and scavenging reactive oxygen. Free Radic Biol Med. 2006; 40:1397-1408. https://doi.org/10.1016/j.freeradbiomed.2005.12.016

Sistema OJS 3.4.0.3 - Metabiblioteca |