Serological evidence of Hepatitis E Virus infection in Antioquia, Colombia slaughtered pigs
Serological evidence of Hepatitis E Virus infection in Antioquia, Colombia slaughtered pigs
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ABSTRACT
Objective. To detect the presence of specific antibodies against Hepatitis E virus (HEV) in pigs slaughtered in Antioquia, the department where the greatest amount of pork is produced and consumed in Colombia. Materials and methods. Between September 2011 and May 2012, blood samples from pigs were obtained in five slaughterhouses of Antioquia, four of them located in the Aburrá Valley subregion and other located in northern subregión. Serum were evaluated with a commercial ELISA kit for diagnosing HEV in humans but adapted to detect IgG and IgM antibodies in pigs. Results. A 100.0% seropositivity for IgG antibodies was found in 1000 samples evaluated, and 82.06% for IgM antibodies were found in 740 samples. Conclusions. These results indicate that pigs in slaughter age in Antioquia, and possibly in Colombia, have been exposed to HEV at some point in their production process and a high percentage of them can arrive to slaughterhouses with recent infection.
RESUMEN
Objetivo. Detectar la presencia de anticuerpos específicos contra el virus de la Hepatitis E (HEV) en cerdos faenados en Antioquia, departamento donde se produce y consume la mayor cantidad de carne de cerdo en Colombia. Materiales y métodos. Entre septiembre de 2011 y mayo de 2012, se obtuvieron muestras de sangre de cerdos en cinco plantas de faenado, cuatro de ellas ubicadas en el Valle de Aburrá y una en la subregión Norte del departamento de Antioquia, las cuales fueron evaluadas mediante un kit de ELISA comercial para diagnóstico de HEV en humanos pero adaptado para la detección de anticuerpos tipo IgG e IgM en cerdos. Resultados. Se encontró una seropositividad de 100.0% para anticuerpos tipo IgG en 1000 muestras evaluadas y de 82.06% para anticuerpos tipo IgM en 740 muestras. Una muestra de heces positiva para la detección del genoma HEV es similar al genotipo 3 encontrada en Estados Unidos. Conclusiones. Estos resultados indican que los cerdos en edad de faenado en Antioquia y posiblemente en Colombia, han tenido exposición a HEV del, un virus zoonótico emergente a nivel mundial, en algún momento de su proceso productivo.
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- Aggarwal R, Naik S. Epidemiology of hepatitis E: current status. J Gastroenterol Hepatol 2009;24(9): 1484-93.
- http://dx.doi.org/10.1111/j.1440-1746.2009.05933.x
- Meng XJ. Zoonotic and foodborne transmission of hepatitis E virus. Semin Liver Dis 2013;33(1):41-49. http://dx.doi.org/10.1055/s-0033-1338113
- Kumar S, Subhadra S, Singh B, Panda BK. Hepatitis E virus: the current scenario. Int J Infect Dis 2013; 17(4):e228-e233.
- http://dx.doi.org/10.1016/j.ijid.2012.11.026
- Kumar A, Beniwal M, Kar P, Sharma JB, Murthy NS. Hepatitis E in pregnancy. Int J Gynaecol Obstet 2004;85(3):240-244.
- http://dx.doi.org/10.1016/j.ijgo.2003.11.018
- Echevarría JM, González JE, Lewis-XimenezLL, Dos Santos DR, Munné MS, Pinto MA,et al.Hepatitis E virus infection in Latin America: a review. J Med Virol 2013; 85(6):1037-1045.
- http://dx.doi.org/10.1002/jmv.23526
- Vitral CL, Alves M, Lewis-Ximenez LL, Khudyakov YE, Lopes dos Santos DR, Gaspar AM. Serological evidence of hepatitis E virus infection in different animal species from the Southeast of Brazil. Mem Inst Oswaldo Cruz 2005;100(2):117–122.
- http://dx.doi.org/10.1590/S0074-02762005000200003
- Cossaboom CM, Córdoba L, Cao D, Ni YY, Meng XJ. Complete genome sequence of hepatitis E virus from rabbits in the United States. J Virol 2012; 86(23):13124-13125
- http://dx.doi.org/10.1128/JVI.02414-12
- Rutjes SA, Lodder-Verschoor F, Lodder WJ, van der Giessen J, Reesink H, Bouwknegt M, et al. Seroprevalence and molecular detection of hepatitis E virus in wild boar and red deer in The Netherlands. J Virol Methods 2010; 168(1-2):197-206.
- http://dx.doi.org/10.1016/j.jviromet.2010.05.014
- Pourpongporn P, Samransurp K, Rojanasang P, Wiwattanakul S, Srisurapanon S. The prevalence of anti-hepatitis E in occupational risk groups. J Med Assoc Thai 2009; 92(Suppl 3):S38–S42
- Ma H, Geng Y, Li Z, Harrison TJ, Huang W, Wang Y. Analysis of the complete genome sequences of one swine and two human hepatitis E virus genotype 4 strains isolated in Beijing, China. Infect Genet Evol 2013; 18:42-47. http://dx.doi.org/10.1016/j.meegid.2013.04.038
- Wang H, He Y, Shen Q, Wang X, Yang S, Cui L, et al. Complete genome sequence of the genotype 4 hepatitis E virus strain prevalent in swine in Jiangsu Province, China, reveals a close relationship with that from the human population in this area. J Virol 2012; 86(15):8334-8335. http://dx.doi.org/10.1128/JVI.01060-12
- Dong C, Meng J, Dai X, Liang JH, Feagins AR, Meng XJ, et al. Restricted enzooticity of hepatitis E virus genotypes 1 to 4 in the United States. J Clin Microbiol 2011; 49(12):4164-4172.
- http://dx.doi.org/10.1128/JCM.05481-11
- Garkavenko O, Obriadina A, Meng J, Anderson DA, Benard HJ, Schroeder BA, et al. Detection and characterisation of swine hepatitis E virus in New Zealand. J Med Virol 2001; 65(3):525–529.
- http://dx.doi.org/10.1002/jmv.2067
- http://dx.doi.org/10.1002/jmv.2067.abs
- Cooper K, Huang FF, Batista L, Rayo CD, Bezanilla JC, Toth TE, et al. Identification of genotype 3 hepatitis E virus (HEV) in serum and fecal samples from pigs in Thailand and Mexico. J Clin Microbiol 2005; 43(4):1684-1688. http://dx.doi.org/10.1128/JCM.43.4.1684-1688.2005
- Seminati C, Mateu E, Peralta B, de Deus N, Martin M. Distribution of hepatitis E virus infection and its prevalence in pigs on commercial farms in Spain. Vet J 2008 175(1):130–132.
- http://dx.doi.org/10.1016/j.tvjl.2006.11.018
- Munné MS, Vladimirsky S, Otegui L, Castro R, Brajterman L, Soto S, et al. Identification of the first strain of swine hepatitis E virus in South America and prevalence of anti-HEV antibodies in swine in Argentina. J Med Virol 2006; 78(12):1579-1583.
- http://dx.doi.org/10.1002/jmv.20741
- Paiva HH,Tzaneva V, Haddad R,Yokosawa J. Molecular characterization of swine hepatitis E virus from southeastern Brazil. Braz J Microbiol. 2007; 38(4):693-698.
- http://dx.doi.org/10.1590/S1517-83822007000400020
- Ibarra VH, Riedemann GS, Reinhardt VG, Calvo AM. Presence of anti hepatitis E virus antibodies in swine: is it an animal reservoir for hepatitis. Rev Med Chil 2007; 135(8):997-1001.
- Kase JA, Correa MT, Luna C,Sobsey MD.Isolation, detection and characterization of swine hepatitis E virus from herds in Costa Rica. Int J Environ Health Res 2008;18(3):165-176.
- http://dx.doi.org/10.1080/09603120701498311
- Agronet.com [sede web] Bogotá: Departamento Administrativo Nacional de Estadística (DANE); 23 de octubre de 2012[Acceso 15 de diciembre de 2013] Boletín mensual INSUMOS Y FACTORES DE PRODUCCIóN: La carne de cerdo en el mundo. URL Disponible en: http://www.agronet.gov.co
- Huang FF, Haqshenas G, Guenette DK,Halbur PG, Schommer SK, Pierson FW, et al. Detection by reverse transcription-PCR and genetic characterization of field isolates of swine hepatitis E virus from pigs in different geographic regions of the United States. J Clin Microbiol 2002; 40(4):1326–1332.
- http://dx.doi.org/10.1128/JCM.40.4.1326-1332.2002
- Leblanc D, Ward P, Gagné MJ, Poitras E, Müller P, Trottier YL, et al. Presence of hepatitis E virus in a naturally infected swine herd from nursery to slaughter. Int J Food Microbiol 2007; 117(2):160-166. http://dx.doi.org/10.1016/j.ijfoodmicro.2007.03.008
- Jacobson RH. Validation of serological assays for diagnosis of infectious diseases. Rev Sci Tech 1998; 17:469-526.
- http://dx.doi.org/10.20506/rst.17.2.1119
- Chernick, MR. The Essentials of Biostatistics for Physicians, Nurses, and Clinicians. New Jersey. John Wiley & Sons. 2011. pp.49–50. http://dx.doi.org/10.1002/9781118071953
- OIE [sede web]. Manual of Diagnostic Tests and Vaccines for Terrestrial Animals 2013.Principles and methods of validation of diagnostic assays for infectious diseases. 2013 [Acceso 15 diciembre 2013] URL Disponible en: http://www.oie.int/fileadmin/Home/fr/Health_standards/tahm/1.01.05_VALIDATION.pdf
- Crowther JR. The ELISA guidebook. 2nd Edition. New York; Human press: 2008.
- Mizuo H, Suzuki K, Takikawa Y, Sugai Y, Tokita H, Akahane Y, et al.Polyphyletic strains of hepatitis E virus are responsible for sporadic cases of acute hepatitis in Japan. J Clin Microbiol 2002;40(9):3209-3218. http://dx.doi.org/10.1128/JCM.40.9.3209-3218.2002
- Díaz CA, Rodríguez MN, Vera VJ, Ramirez G, Casas GA, Mogollón JD. Caracterización de los sistemas de producción porcina en las principales regiones porcícolas colombianas. Rev Colomb Cienc Pecu 2011; 24(2): 131-144.
- de Deus N, Casas M, Peralta B,Nofrarías M, Pina S, Martín M,et al. Hepatitis E virus infection dynamics and organic distribution in naturally infected pigs in a farrow-to-finish farm. Vet Microbiol 2008; 13(1-2):19-28. http://dx.doi.org/10.1016/j.vetmic.2008.04.036
- Yunoki M, Yamamoto S, Tanaka H, Nishigaki H, Tanaka Y, Nishida A, et al. Extent of hepatitis E virus elimination is affected by stabilizers present in plasma products and pore size of nanofilters. Vox Sang 2008; 95(2):94-100.
- http://dx.doi.org/10.1111/j.1423-0410.2008.01078.x
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