Efecto de la edad y la hora de la aspiración sobre la calidad oocitaria y el desarrollo in vitro en hembras bovinas prepúberes

Effect of age and coasting period on oocytes quality and their in vitro development from prepubertal cattle

Objetivo. Este estudio evaluó el efecto de la edad y el período de aspiración folicular so­bre la calidad del oocito y su posterior desarrollo in vitro con donadoras prepúberes Bos indicus mestizas. Materiales y métodos. Las hembras donantes recibieron un implante de norgestomet y benzoato de estradiol y cuatro días más tarde una dosis única de 150 UI de eCG. Las aspiración folicular se realizó en tres tiempos (24, 48, y h 56) y en cuatro edades (3, 6,10, y 12 meses). Todos los folículos antrales fueron aspirados y se realizaron procedimientos de maduración, fertilización y cultivo in vitro. Resultados. Se aspiraron 439 folículos en total, de los cuales 385 (87.7%) fueron de 2-5 mm, 41 (9.33%) fueron de 5-10 mm, y fueron 13 (2.3%) > 10 mm. Se recuperó un total de 373 oocitos (84.9%) y se encontraron diferencias significativas (p<0.05) entre los promedios de los animales de 6 (9.3) y 10 meses (32.3). 285 (76.4%) oocitos fueron sometidos a los procesos in vitro. La tasa de división fue significativamente mayor (p<0.05) en los animales de 10 (27.1%) y 12 meses (26.8%). Aunque el número de embriones transferibles fue bajo, existieron di­ferencias entre las edades (p<0.05), obteniendo el porcentaje más alto los animales de 10 meses (12.6%). Conclusiones. Un período de aspiración superior a 24 h tiene un efecto negativo sobre la calidad del oocito. Algunos oocitos procedentes de terneras de 3 meses fueron competentes en el desarrollo in vitro, sin embargo, un mayor número de embriones se produjeron con hembras de 10 y 12 meses de edad.

1. Chohan KR, Hunter AG. In vitro maturation, fertilization and early cleavage rates of bovine fetal oocytes. Theriogenology 2004; 61:373-380. https://doi.org/10.1016/S0093-691X(03)00220-6
3. Kelly JM, Kleemann DO, Walker SK. A comparision of the in vitrodevelopment of cow and calf oocites. Theriogenology 2003; 59:449(Abstract).
5. Galli C, Duchi R, Crotti G, Turini P, Ponderato N, Colleoni S, Lagutina I, Lazzari G. Bovine embryo technologies. Theriogenology 2003; 59(2):599-616. https://doi.org/10.1016/S0093-691X(02)01243-8
7. Mapletoft RJ, Hasler JF. Assisted reproductive technologies in cattle: a review. Rev Sci Tech Off Int Epiz 2005; 24(1):393-403. https://doi.org/10.20506/rst.24.1.1582
9. Morton KM. Developmental capabilities of embryos produced in vitro from prepubertal lamb oocytes. Reprod Domest Anim 2008; 43(Suppl 2):137-43. https://doi.org/10.1111/j.1439-0531.2008.01153.x
11. Camargo LSA, Viana JHM, Sá WF, Ferreira AM, Vale Filho VR. Developmental competence of ooctytes from prepubertal Bos indicus crossbred cattle. Anim Reprod Sci 2005; 85: 53-59. https://doi.org/10.1016/j.anireprosci.2004.04.020
13. Fisher AE, Bernal DP, Gutierrez-Robayo C, Rutledge JJ. Estimates of heterosis for in vitro embryo production using reciprocal crosses in cattle. Theriogenology 2000; 54:1433-1442. https://doi.org/10.1016/S0093-691X(00)00465-9
15. Camargo LSA, Viana JHM, Sá WF, Ferreira AM, Ramos AA, Freitas C, Vale Filho VR. Developmental competence of oocytes obtained from Bos taurus and Bos indicus dairy cows raised in tropical climate. Reprod Fert Dev 2006; 18:243-244. https://doi.org/10.1071/RDv18n2Ab272
17. Kauffold J, Amer HAH, Bergfeld U, Weber W; Sobiraj A. The In Vitro Developmental Competence of Oocytes from Juvenile Calves is Related to Follicular Diameter. J Reprod Dev 2005; 51(3):325-332. https://doi.org/10.1262/jrd.17002
19. Ptak G, Matsukawa K, Palmieri C, Della Salda L, Scapolo PA, Loi P. Developmental and functional evidence of nuclear immaturity in prepubertal oocytes. Hum Reprod 2006; 21(9):2228-2237. https://doi.org/10.1093/humrep/del184
21. Majerus V, Lequarré AS, Ferguson EM, Kaidi S, Massip A, Dessy F, Donnay I. Characterization of embryos derived from calf oocytes: kinetics of cleavage, cell allocation to inner cell mass, and trophoectoderm and lipid metabolism. Mol Reprod Dev 2000; 57:346-352.2505
23. Bols PEJ, Leroy JLMR, Viana JHM. Technical and biological aspects of ultrasound-guided transvaginal oocyte retrieval in the cow: an overview. Acta Sci Vet 2005; 33(supl1): 103-118.
25. Petyim S, Bage R, Hallap T, Bergqvist AS, Rodriguez-Martinez H, Larsson B. Two different Schemes of twaice wakly ovum pick-up in dairy heifers: effect on oocite recovery and ovarian function. Theriogenology 2003; 60: 175-188. https://doi.org/10.1016/S0093-691X(02)01363-8
27. Merton JS, de Roos APW, Mullaart E, de Ruigh L, Kaal L, Vos PLAM, Dieleman SJ. Factors affecting oocite quality and quantity in comercial application of embryo technologyes in cattle breeding industry. Theriogenology 2003; 59: 651-674. https://doi.org/10.1016/S0093-691X(02)01246-3
29. Taneja M, Bols PEJ, Van de Velde A, Ju JC, Shreiber D, Tripp MW, Levin H, Echelard Y, Yan X. Developmental competence of juvenile calf oocytes in vitro and in vivo: influence of donor animal variation and repeated gonadotropin stimulation. Biol Reprod 2000; 62:206-213. https://doi.org/10.1095/biolreprod62.1.206
31. Presicce GA, Senatore EM, De Santis G, Stecco R, Terzano GM, Borghese A, De Mauro GJ. Hormonal stimulation and oocyte maturational competence in prepuberal Mediterranean Italian buffaloes (Bubalus bubalis). Theriogenology 2002; 57 (7):1877-1884. https://doi.org/10.1016/S0093-691X(02)00677-5
33. Ptak G, Tischner M, Bernabo N, Loi P. Donor-Dependent Developmental Competence of Oocytes from Lambs Subjected to Repeated Hormonal Stimulation. Biol Reprod 2003; 69(1):278-285 https://doi.org/10.1095/biolreprod.102.011312
35. Techakumphu M, Promdireg A, Na-Chiengmai A, Phutikanit N. Repeated oocyte pick up in prepubertal swamp buffalo (Bubalus bubalis) calves after FSH superstimulation. Theriogenology 2004; 61(9):1705-11. https://doi.org/10.1016/j.theriogenology.2003.09.016
37. Chaubal SA, Molina JA, Ohlrichs CL, Ferre LB, Faber DC, Bols PEJ, Riesen JW, Tian X, Yang X. Comparison of different transvaginal ovum pick-up protocols to optimise oocyte retrieval and embryo production over a 10-week period in cows. Theriogenology 2006; 65: 1631-1648. https://doi.org/10.1016/j.theriogenology.2005.07.020
39. Goodhand KL, Staines ME, Hutchinson JSM, Broadbent PJ. In vivo oocyte recovery and in vitro embryo production from bovine oocyte donors treated with progestagen, oestradiol and FSH. Anim Reprod Sci 2000; 63: 145-158. https://doi.org/10.1016/S0378-4320(00)00186-X
41. Blondin P, Bousquet D, Twuagiramungu H, Barnes F, Sirard MA. Manipulation of follicular development to produce developmentally competent Bovine oocytes. Biol Reprod 2002; 66:38-43. https://doi.org/10.1095/biolreprod66.1.38
43. Dieleman SJ, Hendriksen PJM, Viuf D, Thomsen PD, Hyttel P, Knijn HM, Wrenzyck C, Kruip TAM, Niemann H, Gadella BM, Bevers MM, Vos PLAM. Effects of in vivo prematuration and in vivo final maturation on developmental capacity and quality of pre-implantation embryos. Theriogenology 2002; 57: 5-20. https://doi.org/10.1016/S0093-691X(01)00655-0
45. Choi YH, Carnevale EM, Seidel GE Jr, Squire EL. Effects of gonadotropins on bovine oocytes matured in TCM-199. Theriogenology 2001; 56(4):661-70. https://doi.org/10.1016/S0093-691X(01)00597-0
47. Camargo LS, Freitas C, de Sa WF, de Moraes Ferreira A, Serapiao RV, Viana JH. Gestation length, birth weight and offspring gender ratio of in vitro-produced Gyr (Bos indicus) cattle embryos. Anim Reprod Sci 2010; 120(1-4):10-5. https://doi.org/10.1016/j.anireprosci.2010.02.013
49. Seneda MM, Esper CR, Andrade ER, Binelli M, Max MC, Oliveira JA, Garcia JM. Relationship between follicle size after FSH treatment and efficiency of oocytes recovery. Anim Reprod 2005; 2:78-182.
51. Snel-Oliveira MN, Costa PD, Malagoni JD, Rumpf R. Estimulação Hormonal, Punção Folicular Transvaginal e Avaliação Ovocitária em bezerras Pré-Púberes da raça Nelore (Bos taurus indicus). R Bras Zootec 2003; 32: 106-114. https://doi.org/10.1590/S1516-35982003000100014
53. Molina JR, Benoit AM, Lkhagvadorj S, Anderson LL. Hypothalamic differentiation in prepuberal beef heifers: Effects of gonadotropin-releasing hormone and estradiol benzoate on luteinizing hormone secretion. Livest Sci 2009; 120(1):13-24. https://doi.org/10.1016/j.livsci.2008.04.019
55. Oropeza A, Wrenzycki C, Herrmann D, Hadeler KG, Niemann H. Improvement of the Developmental Capacity of Oocytes from Prepubertal Cattle by Intraovarian Insulin-Like Growth Factor-I Application. Biol Reprod 2004; 70(6):1634-1643. https://doi.org/10.1095/biolreprod.103.025494
57. Salamone DF, Damiani P, Fissore RA, Robl JM, Duby RT. Biochemical and developmental evidence the ooplasmic maturation of prepubertal bovine oocytes is compromise. Biol Reprod 2001; 64:1761-1768. https://doi.org/10.1095/biolreprod64.6.1761
59. Mapletoft RJ, Steward KB. Adams GP. Recent advances in the superovulation in cattle. Reprod Nutr Dev 2002; 42(6):601-611. https://doi.org/10.1051/rnd:2002046
61. Peterson AJ, Lee RS. Improving successful pregnancies after embryo transfer. Theriogenology 2003; 59(2):687-97 https://doi.org/10.1016/S0093-691X(02)01248-7