Bacterias endófitas promotoras de crecimiento aisladas de pasto colosoana, departamento de Sucre, Colombia

Endophytes bacterial growth promoters isolated to colosoana grass, Department of Sucre, Colombia

Objetivo. Evaluar in vitro la eficiencia de las bacterias endófitas promotoras de crecimiento aisladas de diferentes tejidos de pasto colosuana en el municipio de Corozal, departamento de Sucre, Colombia. Materiales y métodos. Se aislaron bacterias endófitas, se determinó densidad poblacional en UFC/g de tejido, seguidamente se llevó a cabo pruebas cuantitativas y cualitativas de las actividades de FBN, solubilización de fosfatos, producción de sideróforos y de AIA para finalmente se realizó identificación por secuenciamiento de las aquellas bacterias que tuvieron actividad positiva de promoción de crecimiento. Resultados. Las mayores poblaciones fueron encontradas en raíces (5.0 X 1010 3.8 X 1010 2.8 X 1010 2.4 X 1010 y 1.5 X1010 UFC/g de tejido, para los corregimientos de La Peñas, El Mamón, Cantagallo, Chapinero y Hato Nuevo, respectivamente) con respecto a tallo y hoja. Un total de 53 bacterias endófitas aisladas, 18 mostraron capacidad reductora de N2 a amonio; 15 morfotipos mostraron capacidad solubilizadora de fosfatos; 8 de producción de ácido indol acético y 12 de productoras de sideróforo. Conclusiones. Este trabajo aisló bacterias endófitas con capacidad de promover el crecimiento vegetal. Dos especies de bacterias endófitas fueron identificadas como Delftia tsuruhatensis y Pseudomonas hibiscicola (S. maltophilia), las cuales mostraron excelentes resultados de solubilización de fosfatos, reducen N2 a amonio, producción de ácido índol acético y la producción de sideróforos.

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