Asociación in vitro de Duddingtonia flagrans con ivermectina en el control de nematodos gastrointestinales de búfalos

In vitro association of Duddingtonia flagrans with ivermectin in the control of gastrointestinal nematodes of buffaloes

Objetivo. El objetivo de este estudio fue evaluar la asociación in vitro del hongo Duddingtonia flagrans (AC001) e ivermectina en el control de nematodos gastrointestinales de terneros búfalo. Materiales y métodos. Se formaron cuatro grupos experimentales en microtubos, con cinco réplicas para cada grupo: G1 (nematodos + AC001), G2 (nematodos + ivermectina 1%), G3 (nematodos + AC001 + ivermectina 1%) y G4 (nematodos + agua destilada). Para cada grupo, después de 36 horas de interacción se leyó el contenido de los microtubos mediante microscopía óptica, contabilizando el número de nematodos por grupo. Resultados. Hubo una reducción larvaria significativa de los grupos tratados, con los siguientes porcentajes  con relación al G4 (control): G1: 43,7%; G2: 82,3% y G3: 65,7%. También se observó que la asociación in vitro de D. flagrans con ivermectina fue más efectiva en la reducción de L3 en comparación con el uso aislado de este hongo. Conclusiones. Se concluyó que el uso conjunto de D. flagrans con ivermectina puede potenciar la eficacia del control biológico de los nematodos gastrointestinales de los búfalos, previendo su uso en las condiciones naturales de la cría de búfalos.

1. Mokhber M, Moradi-Shahrbabak M, Sadeghi M, Moradi-Shahrbabak H, Stella A, Nicolzzi E, et al. A genome-wide scan for signatures of selection in Azeri and Khuzestani buffalo breeds. BMC Genom. 2018; 19:449. https://doi.org/10.1186/s12864-018-4759-x
2. Du C, Deng TX, Zhou Y, Ghanem N, Hua GH. Bioinformatics analysis of candidate genes for milk production traits in water buffalo (Bubalus bubalis). Trop Anim Health Prod. 2020; 52(1):63–69. https://doi.org/10.1007/s11250-019-01984-1
3. Nardi Júnior G, RibeiroMG, Vasconcellos SA, Megid J, Jorge AM, Geronutti L, et al. Perfil de aglutininas anti-Leptospira em bezerras búfalas vacinadas com bacterina pentavalente comercial contra leptospirose. Arq Bras Med Vet Zootec. 2006; 58(3):299-304. https://doi.org/10.1590/S0102-09352006000300002
4. Vilela VLR, Feitosa TF, Brasil AWL, Parentoni RN, Bezerra RA, Azevedo SS. Prevalência de parasitas gastrointestinais em búfalos no estado da Paraíba e primeiro relatório de Cystoisospora spp. em búfalos no Brasil. ARS Vet. 2017; 33:26-30. http://doi.org/10.15361/2175-0106.2017v33n1p26-30
5. Bier D, Teruya LS, Borges DGL, Neves JPL, Santos LB, Borges FA.Epidemiology of gastrointestinal helminths in buffaloes. Cienc Anim Bras. 2018; 19:1-9. https://doi.org/10.1590/1809-6891v19e-40882
6. Dantas PCS, Lima DS, Oliveira FJ, Calasans TAS, Porto AG, Carvalho CD, et al. Ocorrência de parasitoses gastrintestinais em vacas leiteiras e respectivos bezerros durante o período de amamentação, na fazenda São Paulino, Município de Itapicuru/BA. SciPlena. 2015; 11(4):046121. https://scientiaplena.org.br/sp/article/view/2491/1185
7. Braga FR, AraújoJV. Nematophagous fungi for biological control of gastrointestinal nematodes in domestic animals. Appl Microbiol Biotechnol. 2014; 98(1):71-82. http://doi.org/10.1007/s00253-013-5366-z
8. Vilela VLR, Feitosa TF, Braga FR, Santos A, Bezerra RA, Silva GLL, et al.Use of Duddingtonia flagrans in the control of gastrointestinal nematodes of feedlot goats. Semina: Cienc Agrár. 2020; 41(3):915-924. http://doi.org/10.5433/1679-0359.2020v41n3p915
9. Braga FR, Ferraz CM, Silva EM, Araújo JV.Efficiency of the Bioverm® (Duddingtonia flagrans) fungal formulation to control in vivo and in vitro of Haemonchus contortus and Strongyloides papillosus in sheep. 3 Biotech. 2020; 10(2):1-5. http://doi.org/10.1007/s13205-019-2042-8
10. Soares FB, Monteiro AC. Compatibilidade de Metarhizium anisopliae com carrapaticidas químicos. Arq Inst Biol. 2011; 78:385-391. http://doi.org/10.1590/1808-1657v78p3852011
11. Ferraz CM, Sobral SA, Senna CC, Junior OF, Moreira TF, Tobias FL, et al. Combined use of ivermectin, dimethyl sulfoxide, mineral oil and nematophagous fungi to control Rhabditis spp. Vet Parasitol. 2019; 275:108924. http://doi.org/10.1016/j.vetpar.2019.108924
12. Araújo JV, Maia AS. Antagonistic effect of predatory fungi Arthrobotrys on infectious Haemonchus placei larvae. J Helminthol. 1993; 67(2):136-138. https://doi.org/10.1017/S0022149X00013018
13. Gordon HM, Whitlock HV. A new technique for counting nematode eggs in sheep faeces. J Sci Ind Res. 1939; 12(1):50-52. http://hdl.handle.net/102.100.100/339340?index=1
14. Roberts FHS, O’Sullivan JP. Methods of egg counts and larval cultures for Strongyles infesting the gastrointestinal tract of cattle. Aust J Agr Res. 1950;1(1):99-102. http://doi.org/10.1071/AR9500099
15. Willcox HP, Coura JR. Nova concepção para o método de Baermann-Moraes-Coutinho na pesquisa de larvas de nematóides. Mem Inst Oswaldo Cruz. 1989; 84(4):563–565. https://doi.org/10.1590/S0074-02761989000400015
16. Keith RK. Differentiation of infective larval of some common nematode parasites ofcattle. Aust J Zool. 1953; 1(2):223–235. https://doi.org/10.1071/ZO9530223
17. Ayres M, Ayres JRM, Ayres DL, Santos AS.BioEstat 5.0: aplicações estatísticas nas áreas de ciências biológicas e médicas. 2007. 4nd ed. Belém: Sociedade Civil Mamirauá. https://www.researchgate.net/profile/Alex-De-Assis-Dos-Santos-2/publication/263608962_BIOESTAT_-
18. _aplicacoes_estatisticas_nas_areas_das_Ciencias_Bio-Medicas/links/02e7e53b598e69ebfe000000/BIOESTAT-aplicacoes-estatisticas-nas-areas-das-Ciencias-Bio-Medicas.pdf
19. Mendoza-De Gives P, Vazquez-Prats VM. Haemonchus contortus reduction infective larvae by three nematophagous fungi in fecal sheep cultures. Vet Parasitol. 1994; 5(3):197-203. https://doi.org/10.1016/0304-4017(93)00646-G
20. Ojeda-Robertos NF, Aguilar-Marcelino L,Olmedo-Juárez A, Luna-Palomera C, Peralta-Torres JA,López-Arellano MA, et al. In vitro predatory activity of nematophagous fungi isolated from water buffalo feces and fromsoil in the Mexican south eastern. Rev Bras Parasitol Vet. 2019; 28(2):314-319. https://doi.org/10.1590/s1984-29612019011
21. Barroga TRM, Collantes TMA, Mingala CN. Larvicidal activity of nematophagous fungi Duddingtonia flagrans against common strongyle roundworms of buffaloes (Bubalus bubalis). Philipp J Vet Anim Sci. 2016; 42(1):49-58. http://pjvas.org/index.php/pjvas/article/download/136/129
22. Vilela VLR, Feitosa TF, Braga FR, Vieira VD, Lucena SC, Araújo JV. Control of gastrointestinal nematodes in sheep using the combination of Duddingtonia flagrans and Levamisole Hydrochloride 5%. Ver Bras Parasitol Vet. 2018; 27(1):23-31. https://doi.org/10.1590/s1984-29612018008
23. Sanyal PK, Chauhan JB, Mukhopadhyaya PN. Implications of Fungicidal Effects of benzimidazole compounds on Duddingtonia flagrans in integrated nematode parasite management in livestock. Vet Res Commun. 2004; 28(5):375-385. http://doi.org/10.1023/B:VERC.0000034997.50332.77
24. Vieira JN, Filho FSM, Ferreira GF, Mendes JF, Gonçalves CL, Villela MM, et al. In vitro susceptibility of nematophagous fungi to antiparasitic drugs: interactions and implications for biological control. Braz J Biol. 2017; 77(3):476-479. https://doi.org/10.1590/1519-6984.15715
25. Sobral SA, Ferreira BS, Senna CC, Ferraz CM, Moreira TF, Junior OFL. Rhabditis spp., in the Espírito Santo, State of Brazilan devaluation of biological control. Ver Bras de Parasitol Vet. 2019; 28(2):333-337. https://doi.org/10.1590/s1984-29612019020
26. Hirose E, Neves PMOJ, Zequi JAC, Martins LH, Peralta CH, Moino Junior A. Efeito de biofertilizantes e óleo de nim sobre os fungos entomopatogênicos Beauveria bassiana (Bals.) Vuill. e Metarhizium anisopliae (Metsch.) Sorok. Arq Bras Biol Tecnol. 2001; 44:419-423. http://doi.org/10.1590/S1516-89132001000400013
27. Ferreira GF, Freitas TM, Gonçalves CL, Mendes JF, Vieira JN, Villareal JP, et al. Antiparasitic drugs: in vitro tests against nematophagous fungi. Braz J Biol. 2016; 76(4):990-993. http://doi.org/10.1590/1519-6984.05615
28. Burke JM, Miller JE. Sustainable approaches to parasite control in ruminant live stock. Vet Clin North Am Food Anim Pract. 2020; 36:89-107. http://doi.org/10.1016/j.cvfa.2019.11.007
29. Szewc M, De Waal T, Zintl A. Biological methods for the control of gastrointestinal nematodes. J Vet. 2021; 268:105602. http://doi.org/ 10.1016/j.tvjl.2020.105602
30. Minguetto JGM, Bogado ALG, OkanoW, Cunha filho LFC, Silva LC, Zanol D, Ferraz CM, Moreira TF, Tobias FL, Braga FR, Araújo JV. Biological control of gastrointestinal nematodes in Young ewestreated with fungi. Biocontrol Sci Technol. 2021;31(5):499-511. https://doi.org/10.1080/09583157.2020.1869699