Evaluación de mesófilos de lactosuero con actividad ácido láctica en medio con Canna indica L

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Dania Fonseca-López
Carlos Eduardo Rodriguez Molano
Lily Monroy-Fonseca
Resumen

Objetivo. Evaluar lactosuero como fuente de mesófilos con actividad ácido láctica en medio de cultivo complejo con almidón de sagú (Canna indica L.). Materiales y métodos. Se analizaron tres medios de cultivo para bacterias mesófilas de lactosuero diferenciados por la inclusión de almidón de sagú (Canna indica L.) (0, 0.5 y 1%). Los tratamientos control fueron suero refrigerado (-4°C) y sin refrigerar (18°C). Se evaluó el crecimiento de mesófilos, pH, nivel de acidez, y sólidos solubles totales con intervalos de 24 h durante 5 días; y concentración de ácido láctico al finalizar el ensayo. Resultados. El pH y los sólidos solubles totales (°Brix) disminuyeron en todos los tratamientos, el porcentaje de acidez aumentó con el tiempo y al finalizar el ensayo el mayor contenido de ácido láctico fue de 32.5 y 37.2 g/L para las formulaciones con 0.5 y 1% de almidón de sagú respectivamente. El crecimiento de mesófilos fue bajo en la formulación con 1% de almidón y alto en el suero sin refrigerar. Conclusión. El lactosuero es una buena fuente de mesófilos con actividad ácido láctica lográndose hasta 37.2 g/L de ácido láctico en medio de cultivo complejo con inclusión de 1% de almidón de sagú.

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