Examen de adenovirus con métodos moleculares y patológicos en casos de pneumonía ovina

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Resumen

Objetivo. Revelar adenovirus (AdV) que causan pneumonía en ovejas y examinar cambios patológicos en los ganglios linfáticos pulmonares y mediastínicos de muestras positivas para adenovirus infectadas de forma natural. Material y métodos. Se examinaron macroscópicamente 1459 pulmones de ovejas y se detectaron lesiones de pneumonía en 88 (6.03%) de estas. Las secciones de tejido parafinadas de estos especímenes con pneumonía se examinaron con los métodos inmunohistoquímicos (IHC) e inmunofluorescencia indirecta (IF), mientras que tejidos homogeneizados se examinaron usando los métodos ELISA de antígeno y PCR para determinar la positividad de adenovirus. Resultados. La prevalencia de adenovirus se determinó como 19.3% para IHC, 22.7% para IF, 20.5% para ELISA y 13.6% para PCR. La tinción con hematoxilina-eosina (HE) se realizó para examinar los cambios histopatológicos en las muestras que estaban infectadas naturalmente con adenovirus. Los exámenes histopatológicos de las muestras de pulmón infectadas de forma natural revelaron mayormente pneumonía intersticial, junto con hallazgos de pneumonía catarral y verminosa. Se determinó que los métodos más eficaces en la detección de adenovirus en pneumonías ovinas fueron encontrado respectivamente como IF, ELISA, IHC y PCR. El hallazgo de que los adenovirus solo se vio en los ganglios linfáticos mediastínicos de algunas muestras en los métodos inmunopatológicos sugirió latencia. Conclusiones. La presencia de adenovirus en casos de pneumonía ovina se determinó por primera vez con los métodos de inmunofluorescencia indirecta, ELISA de antígenos y PCR. La posibilidad de la naturaleza latente de la infección por adenovirus en estas especies también se discutió por primera vez.

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Biografía del autor/a / Ver

Muhammet Bahaeddin Dörtbudak, Bingol University, Turquía

Bingol University Faculty of Veterinary Medicine, Department of Veterinary Pathology, Bingol, Turkey.

Yavuz Selim Sağlam, Atatürk University, Turquía

Atatürk University Faculty of Veterinary Medicine, Department of Veterinary Pathology, Erzurum, Turkey.

Serkan Yıldırım, Atatürk University, Turquía

Atatürk University Faculty of Veterinary Medicine, Department of Veterinary Pathology, Erzurum, Turkey.

Mehmet Özkan Timurkan, Atatürk University, Turquía

Atatürk University Faculty of Veterinary Medicine, Department of Veterinary Virology, Erzurum, Turkey.

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