Calidad microbiológica y actividad antibacteriana de miel producida por Melipona beecheii en Yucatán, México
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Objetivo. Analizar la calidad microbiológica y actividad antibacteriana en 43 muestras de miel producida por Melipona beecheii, extraída durante las épocas de cosecha (enero a mayo del 2020 y 2021) y poscosecha (junio a octubre 2020) de meliponarios ubicados en selva baja caducifolia del Sureste de México. Materiales y métodos. La calidad microbiológica se determinó evaluando el contenido de mesófilos aerobios totales, coliformes, hongos, levaduras y anaerobios formadores de esporas. Para la actividad antibacteriana, se utilizó el ensayo de difusión en agar con pocillos utilizando concentraciones de miel al 5, 10, 20, 40 y 80%. Resultados. Se observó la presencia de mesófilos aerobios (83.7% de las muestras), coliformes (4.6%), mohos (20.9%) y levaduras (39.5%), con un máximo de 4.5x102, 2.5x10, 9.5x10 y 3.2x103 UFC/g, respectivamente. En ninguna muestra se observó la presencia de formas esporuladas de clostridios sulfito reductores. Con respecto a la actividad antibacteriana las mayores zonas de inhibición se registraron contra Staphylococcus aureus a una concentración de la miel al 80 y 40%, contrario a lo observado con Salmonella var. Typhimurium, Pseudomonas aureginosa y Escherichia coli, en donde la interferencia en el crecimiento bacteriano no fue tan evidente. Conclusiones. No obstante, el crecimiento de mesófilos y levaduras en la mayoría de las muestras, éstas presentaron actividad antibacteriana contra los patógenos referidos, lo cual puede ser atribuido a las interacciones entre microbioma, plantas, abejas y características fisicoquímicas de la miel.
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Bratman EZ. Saving the Other Bees: The Resurgence of Stingless Beekeeping in the Zona Maya. Conserv Soc. 2020; 18(4):387-398. https://doi.org/10.4103/cs.cs_20_66
Marconi M, Ormeño-Luna J, Vecco-Giove CD. Physicochemical and microbiological quality of honeys produced by stingless bees Scaptotrigona polysticta, Melipona illota and Tetragonisca angustula (Apidae: Meliponini) in San Martín, Peru. Peruv J Agron. 2020; 4(2): 55-60. https://doi.org/10.21704/pja.v4i2.1541
Pinheiro CG, Abrantes MR, Silva RO, Junior CA, Lobato FC, Silva JB. Microbiological quality of honey from stingless bee, jandaíra (Melipona subnitida), from the semiarid region of Brazil. Ciênc Rural. 2018; 48(9):e20180151. https://doi.org/10.1590/0103-8478cr20180151
Ngalimat MS, Raja Abd Rahman RNZ, Yusof MT, Syahir A, Sabri S. Characterisation of bacteria isolated from the stingless bee, Heterotrigona itama, honey, bee bread and propolis. Peer J. 2019; 7:e7478. https://doi.org/10.7717/peerj.7478
Vit P, Medina M, Enríquez ME. Quality standards for medicinal uses of Meliponinae honey in Guatemala, Mexico and Venezuela. Bee World. 2004; 85(1):2-5. https://doi.org/10.1080/0005772X.2004.11099603
Rao PV, Krishnan KT, Salleh N, Gan SH. Biological and therapeutic effects of honey produced by honey bees and stingless bees: a comparative review. Rev Bras Farmacogn. 2016; 26(5):657-664. https://doi.org/10.1016/j.bjp.2016.01.012
Domingos SCB, Clebis VH, Nakazato G, de Oliveira AGJr, Takayama Kobayashi RK, Peruquetti RC, et al. Antibacterial activity of honeys from Amazonian stingless bees of Melipona spp. and its effects on bacterial cell morphology. J Sci Food Agric. 2021; 101(5):2072-2077. https://doi.org/10.1002/jsfa.10828
Brown E, O'Brien M, Georges K, Suepaul S. Physical characteristics and antimicrobial properties of Apis mellifera, Frieseomelitta nigra and Melipona favosa bee honeys from apiaries in Trinidad and Tobago. BMC Complement Altern Med. 2020; 20(1):85. https://doi.org/10.1186/s12906-020-2829-5
Ng WJ, Sit NW, Ooi PA, Ee KY, Lim TM. The antibacterial potential of honeydew honey produced by stingless bee (Heterotrigona itama) against antibiotic resistant bacteria. Antibiotics (Basel). 2020; 9(12):871. https://doi.org/10.3390/antibiotics9120871.
Albaridi NA. Antibacterial potency of honey. Int J Microbiol Res. 2019; 2: 2464507. https://doi.org/10.1155/2019/2464507.
Brudzynski K. Honey as an ecological reservoir of antibacterial compounds produced by antagonistic microbial interactions in plant nectars, honey and honey bee. Antibiotics. 2021; 10(5):551. https://doi.org/10.3390/antibiotics10050551
Almasaudi S. The antibacterial activities of honey. Saudi J Biol Sci. 2021; 28(4):2188-2196. https://doi.org/10.1016/j.sjbs.2020.10.017.
Castillo CAV, Moguel OYB, Cortés CMA, Espinosa HA, Arechavaleta VME, Mora AMA. Composición botánica de mieles de la península de Yucatán, mediante qPCR y análisis de curvas de disociación. Rev Mex Cienc Pecu. 2016; 7(4):489–505. https://doi.org/10.22319/rmcp.v7i4.4278
Zamudio AC. Producción de miel convencional y orgánica en la península de Yucatán. (Tesis de maestría). México: El Colegio de la Frontera Sur; 2017. https://ecosur.repositorioinstitucional.mx/jspui/handle/1017/1932
Tanuğur-Samanc AE, Kekeçoğlu M. An evaluation of the chemical content and microbiological contamination of Anatolian bee venom. PLoS One. 2021; 16(7):e0255161. https://doi.org/10.1371/journal.pone.0255161
Trinks F. Análisis microbiológico de los alimentos. Metodología analítica oficial. Microorganismos indicadores. Volumen 3. ReNaLOA-ANMAT: Argentina; 2014. http://www.anmat.gov.ar/renaloa/docs/analisis_microbiologico_de_los_alimentos_vol_iii.pdf
Ramírez-Miranda I, Betancur-Ancona D, Moguel-Ordóñez Y. Physicochemical and microbiological standards of honey produced by genus Melipona. J Apic Sci. 2021; 65(2):197-216. https://doi.org/10.2478/jas-2021-0016
Caldas MJM, Silva IP, Machado CS, Carvalho CAL, Sodré G da S. Qualidade e perfil antimicrobiano do mel de Melipona asilvai. Braz J Dev. 2020; 6(5):32760–32768. http://dx.doi.org/10.34117/bjdv6n5-646
Fernandes RT, Rosa IG, Conti-Silva AC. Microbiological and physical-chemical characteristics of honeys from the bee Melipona fasciculata produced in two regions of Brazil. Ciência Rural. 2018; 48(5): e20180025. https://doi.org/10.1590/0103-8478cr20180025
Nadja JW, Ajit A, Naila A, Sulaiman AZ, Naila A. Physicochemical and microbiological analysis of stingless bees honey collected from local market in Malaysia. Indones J Chem. 2019; 19(2):522-530. https://doi.org/10.22146/ijc.40869
Vázquez-Quiñones CR, Moreno-Terrazas R, Natividad-Bonifacio I, Quiñones-Ramírez EI, Vázquez-Salinas C. Microbiological assessment of honey in México. Rev Argent Microbiol. 2018; 50(1):75-80. https://doi.org/10.1016/j.ram.2017.04.005.
Schencke C, Vásquez B, Sandoval C, del Sol M. El rol de la miel en los procesos morfofisiológicos de reparación de heridas. Int. J. Morphol 2016; 34(1):385-395. https://dx.doi.org/10.4067/S0717-95022016000100056
Abdulla CO, Ayubi A, Zulfiquer F, Santhanam G, Ahmed MA, Deeb J. Infant botulism following honey ingestion. BMJ Case Rep. 2012: bcr1120115153. https://doi.org/10.1136/bcr.11.2011.5153
Silva MS, Rabadzhiev Y, Eller MR, Ilia Iliev I, Ivanova I, Santana WC. Microorganisms in Honey. In (Ed.), Honey Analysis. IntechOpen. 2017. http://dx.doi.org/10.5772/67262
Beux MR, Ávila S, Surek M, Bordin K, Leobet J, Barbieri F et al. Microbial biodiversity in honey and pollen pots produced by Tetragonisca angustula (Jataí). Biol Appl Sci. 2022; 65:e22210440. https://doi.org/10.1590/1678-4324-2022210440
Santos AC, Biluca FC, Braghini F, Gonzaga LV, Oliveira Costa AC, Fett R. Phenolic composition and biological activities of stingless bee honey: An overview based on its aglycone and glycoside compounds. Food Res Int. 2021; 147(2021):110553. https://doi.org/10.1016/j.foodres.2021.110553.
Silhavy TJ, Kahne D, Walker S. The bacterial cell envelope. Cold Spring Harb Perspect Biol. 2010; 2(5):a000414. https://doi.org/10.1101/cshperspect.a000414.
Chan-Rodríguez D, Ramón-Sierra J, Lope-Ayora J, Sauri-Duch E, Cuevas-Glory L, Ortiz-Vázquez E. Antibacterial properties of honey produced by Melipona beecheii and Apis mellifera against foodborn microorganisms. Food Sci Biotechnol. 2012; 21(3):905-909. https://doi.org/10.1007/s10068-012-0118-x
Dória MM, Dória PE, Trovatti UAP, Lucchese AM. Antimicrobial activity of honey from five species of Brazilian stingless bees. Ciênc Rural. 2013; 43(4):672-675. https://doi.org/10.1590/S0103-84782013005000016
Salminen JP, Roslin T, Karonen M, Sinkkonen J, Pihlaja K, Pulkkinen P. Seasonal variation in the content of hydrolyzable tannins, flavonoid glycosides, and proanthocyanidins in oak leaves. J Chem Ecol. 2004; 30(9):1693-1711. https://doi.org/10.1023/b:joec.0000042396.40756.b7
Bouarab-Chibane L, Forquet V, Lantéri P, Climent Y, Léonard-Akkari L, Oulahal N et al. Antibacterial Properties of Polyphenols: Characterization and QSAR (Quantitative Structure-Activity Relationship) Models. Front Microbiol. 2019; 10:829. https://doi.org/10.3389/fmicb.2019.00829
Ruiz-Ruiz JC, J. Matus-Basto AJ, Acereto-Escoffié P, Segura-Campos MR. Antioxidant and anti-inflammatory activities of phenolic compounds isolated from Melipona beecheii honey. Food Agric Immuno. 2017; 28:1424-1437. https://doi.org/10.1080/09540105.2017.1347148
Alvarez SM, Giampieri F, Brenciani A, Mazzoni L, Gasparrini M, González AM, et al. Apis mellifera vs Melipona beecheii Cuban polifloral honeys: A comparison based on their physicochemical parameters, chemical composition and biological properties. LWT - Food Sci. Technol. 2018; 87:272-279. https://doi.org/10.1016/j.lwt.2017.08.079
Lee H, Churey JJ, Worobo RW. Antimicrobial activity of bacterial isolates from different floral sources of honey. Int J Food Microbiol. 2008; 126(1-2):240-244. https://doi.org/10.1016/j.ijfoodmicro.2008.04.030
Rosli FN, Hazemi MHF, Akbar MA, Basir S, Kassim H, Bunawan H. Stingless bee honey: Evaluating its antibacterial activity and bacterial diversity. Insects. 2020; 11(8):500. https://doi.org/10.3390/insects11080500
