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Cultivo de células epiteliales dentales: impacto del suero fetal bovino

Culture of dental epithelial cells: impact of fetal bovine serum



Cómo citar
Simancas-Escorcia, V. H., Martinez-Martinez, A., & Díaz-Caballero, A. (2020). Cultivo de células epiteliales dentales: impacto del suero fetal bovino. Revista MVZ Córdoba, 25(2), e1639. https://doi.org/10.21897/rmvz.1639

Dimensions
PlumX
Victor Hugo Simancas-Escorcia
Adel Martinez-Martinez
Antonio Díaz-Caballero

Objetivo. Describir la influencia del Suero Fetal Bovino (SFB) en la supervivencia, crecimiento y expresión de organelas celulares en las células epiteliales dentales de rata. Materiales y métodos. Cultivos de células epiteliales dentales de rata fueron llevados a cabo a 37°C en una atmosfera húmeda, en ausencia y a una concentración de 10% de SFB. Una evaluación morfológica fue realizada durante la proliferación y confluencia de las células en cultivo. Dobles marcajes por inmunofluorencia fueron efectuados haciendo uso de anticuerpos anti-actina, anti-TOMM20 y anti-LAMP1. Resultados. Se evidenciaron células epiteliales dentales circulares u ovoides con núcleos voluminosos durante la proliferación y confluencias de manera similar en las células cultivas en presencia y ausencia de SFB. La carencia de SFB impactó negativamente la proliferación de las células epiteliales. No fueron observadas alteraciones en la localización de los inmunomarcajes anti-actina, anti-TOMM20 y anti-LAMP1 en las dos condiciones de cultivos experimentales. Conclusiones. La supresión del SFB en el cultivo de células epiteliales dentales de rata disminuyó la supervivencia, proliferación y sugiere no tener un impacto sobre las organelas evaluadas.


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