Effect of glucose concentration on sperm motility activation in bocachico Prochilodus magdalenae (Pisces, Characiformes)

Efectos de la concentración de glucosa sobre la activación de la movilidad espermática en bocachico Prochilodus magdalenae (Pisces, Characiformes)

Published 2011-05-10

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Martínez, G., Atencio G, V., & Pardo C, S. (2011). Effect of glucose concentration on sperm motility activation in bocachico Prochilodus magdalenae (Pisces, Characiformes). Journal MVZ Cordoba, 16(2), 2554-2563. https://doi.org/10.21897/rmvz.1020
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https://doi.org/10.21897/rmvz.1020
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Gregorio Martínez
Universidad Nacional de Colombia, FCA/DPA, Grupo de Investigación en Biodiversidad y Genética Molecular - BIOGEM, Sede Medellín.
Víctor Atencio G
Universidad de Córdoba, FMVZ/DCA, Centro de Investigación Piscícola – CINPIC
Sandra Pardo C
Universidad Nacional de Colombia, FCA/DPA, Grupo de Investigación en Biodiversidad y Genética Molecular - BIOGEM, Sede Medellín.
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Objective. To determine the concentration of non-activating glucose on sperm motility as a component of future extender for cryopreservation of Prochilodus magdalenae semen. Materials and methods. Inactive semen obtained by hormonal induction from 3 males was analyzed. From each male, 0.25 μL of semen was deposited on Makler’s chamber, and then a fixed amount of 75 μl of glucose solution was added to each drop given a final concentration of, 0, 1, 2, 3, 4, 5, 6, 7, 8, 9 and 10%. Each male was checked 3 times (n=3). Osmolarity was determined in each seminal sample (~250-300 mOsm/Kg) and into each solution, (0, 62, 124, 186, 250, 310, 360, 410, 472, 536 y 620 mOsm/kg, respectively. The software Sperm Class Analyzer (SCA) was used to determine the curvilinear (VCL μm/sec) and straight (VSL, μm/sec) velocity (VSL, μm/sec), likewise, the rate of sperm motility (rapid, medium, and slow) was determined. Rate of immotile sperm was also determined. The activation time of motility (seconds) was obtained under light-field microscope. Results. Glucose Concentrations of 0 and 5% caused activation of sperm motility. Sperm activation was not visually detected in samples with glucose concentrations of 6% or higher. However, the SCA detected a total sperm motility of 7.2%, a VCL of 5.1 μm/sec and a VSL of 1.7 μm/sec. The SCA detected 100% non motile sperm in samples with 7% glucose solution and the motility was not subsequently acquired when distilled water was added. Conclusions. Glucose concentration has a determining effect on viability and activation of fresh sperm. Concentration of 6% glucose solution can be use as a non-activating solution for sperm motility in extenders for sperm cryopreservation in this specie.

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