Skip to main navigation menu Skip to main content Skip to site footer

Prevalence of Salmonella spp., in mesenteric pig’s ganglia at Colombian benefit plants

Prevalencia de Salmonella spp., en ganglios mesentéricos de porcinos en plantas de beneficio Colombianas



How to Cite
Ayala-Romero, C., Ballen-Parada, C., Rico-Gaitan, M., Chamorro-Tobar, I., Zambrano-Moreno, D., Poutou-Piñales, R., & Carrascal-Camacho, A. (2018). Prevalence of Salmonella spp., in mesenteric pig’s ganglia at Colombian benefit plants. Journal MVZ Cordoba, 23(1), 6447-6486. https://doi.org/10.21897/rmvz.1242

Dimensions
PlumX
Carlos Ayala-Romero
Carlos Ballen-Parada
Mónica Rico-Gaitan
Ileana Chamorro-Tobar
Diana Zambrano-Moreno
Raúl Poutou-Piñales
Ana Carrascal-Camacho

Objective. Objective was to determine the prevalence of Salmonella spp., in pigs mesenteric ganglion, from different regions of Colombia. Materials and Methods. A stratified sampling by proportional fixation was carried out at benefit plants of each of the 13 participating departments, whose pork production volume is representative at national level. Sampling was performed during five months, for a total of 457 samples analyzed. Salmonella spp., identification was performed by the MDS Molecular System, later isolates were confirmed in Maldi-TOF MS. Antimicrobial susceptibility of the isolates was determined using the B1016-180 panel and statistical analysis was performed in Whonet 2016, some of the multi-resistant isolates were them serotyped by Kauffman-White method. Results. National prevalence was 28.2%, with the presence of S. typhimurium, S. Agama, S. London, S. Agona, S. Haifa and S. 1,4,12: i: -. Resistance to antibiotics frequently used in human (23.6% Trimethoprim/Sulfamethoxazole, 2.7% Cefotaxime (CTX), 11.8% Ampicillin (AMP) and 1.8% Ciprofloxacin) was found. Conclusion. The prevalence of Salmonella in mesenteric ganglia was 28.2%, being the Huila region the one with the highest prevalence, recovering atypical serotypes such as S. London and S. Haifa.


Article visits 3009 | PDF visits


Downloads

Download data is not yet available.
  1. Scallan E, Hoekstra RM, Angulo FJ, Tauxe RV, Widdowson M-A, Roy SL et al. Foodborne Illness Acquired in the United States—Major Pathogens. Emerg Infect Dis 2011; 17(1):7-15. https://doi.org/10.3201/eid1701.P21101
  2. Binter C, Straver JM, Häggblom P, Bruggeman G, Lindqvist PA, Zentek J et al. Transmission and control of Salmonella in the pig feed chain: a conceptual model. Int J Food Microbiol 2011; 145(S1):S7-17. https://doi.org/10.1016/j.ijfoodmicro.2010.09.001
  3. Davies PR. Intensive Swine Production and Pork Safety. 2011; 8(2):189-201.
  4. Arguello H, Àlvarez-Ordo-ez A, Carbajal A, Rubio P, Prieto M. Role of slaughtering in Salmonella spreading and control in pork production. J Food Prot 2013; 76(5):899-911. https://doi.org/10.4315/0362-028X.JFP-12-404
  5. Rondón-Barragán IS, Rodríguez GA, Marín M GA. Determinación de la seroprevalencia de Salmonella spp. en granjas porcinas del departamento del Tolima. Orinoquia 2014; 18(1):60-67. https://doi.org/10.22579/20112629.281
  6. Nollet N, Maes D, Duchateau L, Hautekiet V, Houf K, Van Hoof J et al. Discrepancies between the isolation of Salmonella from mesenteric lymph nodes and the results of serological screening in slaughter pigs. Vet Res 2005; 36:545-555. https://doi.org/10.1051/vetres:2005014
  7. Bahnson PB, Kim J-Y, Weigel RM, Miller GY, Troutt HF. Associations between on-farm and slaughter plant detection of Salmonella in market-weight pigs. J Food Prot 2005; 68(2):246-250. https://doi.org/10.4315/0362-028X-68.2.246
  8. Pulecio-Santos S, Bermúdez-Duarte P, Suárez-Alfonso MC. Susceptibilidad antimicrobiana de aislamientos de Salmonella enterica obtenidos del pre-beneficio y de porcinos en Colombia. Rev Salud Public 2015; 17(1):106-119. https://doi.org/10.15446/rsap.v17n1.45716
  9. Clinical and Laboratory Standards Institute. Performance Standards for Antimicrobial Susceptibility Testing (M100-S27). 27th edition. CLSI: USA; 2016.
  10. European Food Safety Authority (EFSA). Report of the task force on zoonoses data collection on the analysis of the baseline survey on the prevalence of Salmonella in slaughter pigs, in the EU, 2006-2007. Part A: Salmonella prevalence estimates. The EFSA J 2008; 136:1-11.
  11. Robinault C, Houdayer C, Rouxel S, Labbé A, Tircot A, Denis M et al. Estimation de la prévalence de l'infection par Salmonella spp des porcs charcutiers français à l'abattoir. 2008; 40:49-50.
  12. Talavera Rojas M. Análisis epidemiológico molecular de Salmonella spp. y su relación con la resistencia antibiótica en cerdos de abasto en rastrtos del Valle de Toluca, México. [Ph.D. Thesis] Colima: Universidad de Colima; 2004.
  13. De Busser EV, Maes D, Houf K, Dewulf J, Imberechts H, Bertrand S et al. Detection and characterization of Salmonella in lairage, on pig carcasses and intestines in five slaughterhouses. Int J Food Microbiol 2011; 145(1):279-286. https://doi.org/10.1016/j.ijfoodmicro.2011.01.009
  14. Asociación Colombiana de Porcicultores, Fondo Nacional de la Porcicultura. Informe de los proyectos de inversión desarrollados durante el primer semestre del 2014. 2014: 341p.
  15. Díaz CA, Rodríguez MN, Vera VJ, Ramírez G, Casas GA, Mogollón JD. Characterization of pig farms in the main swine producing regions of Colombia. Rev Col Cienc Pec 2011; 24:131-144.
  16. Zapata JF, Vergara LA, Cuervo CM. Detección de bacterias del género Salmonella sp. en matadero de cerdos de un municipio de Antioquia. Rev Facult Cienc Foren Sal 2012; 8:73-77.
  17. Duggan SJ, Mannion C, Prendergast DM, Leonard N, Fanning S, Gonzales-Barron U et al. Tracking the Salmonella status of pigs and pork from lairage through the slaughter process in the Republic of Ireland. J Food Prot 2010; 73(2):2148-2160. https://doi.org/10.4315/0362-028X-73.12.2148
  18. Mulder RWAW. Impact of transport and related stresses on the incidence and extent of human pathogens in pigment and poultry J Food Saf 1995; 15(2):239-246. https://doi.org/10.1111/j.1745-4565.1995.tb00136.x
  19. Baer AA, Miller MJ, Dilger AC. Pathogens of interest to the pork industry: A review of research on interventions to assure food safety. Compr Rev Food Sci Food Saf 2013; 12:183-217. https://doi.org/10.1111/1541-4337.12001
  20. Bermúdez D PM, Rincón G SM, Suárez A MC. Evaluación de la susceptibilidad antimicrobiana de cepas de Salmonella spp. aisladas del beneficio porcino en Colombia. Rev Fac Nac Salud Pública 2014; 32(1):88-94.
  21. Pineda Y, de Aponte F, Santander J. Aislamiento de Salmonella sp. de origen porcino y su susceptibilidad in vitro a los antimicrobianos. Rev Vet Trop 2001; 26(1):63-76.
  22. Ibarra Gómez F, Bascopé Maida SC, Yerko BA, Bejarano Forqueras HA, Bustamante Butrón RC, Cadima Terrazas MA et al. Sencibilidad y resistencia de las salmonelas a los antimicrobianos en la ciudad de Cochabamaba. Gac Med 2005:3-7.
  23. Gutiérrez M, Granda A, Bonachea H. Determinación de la sensibilidad antimicrobiana en cepas de Salmonella enterica subsp. enterica, aisladas de alimentos. Rev Cub Cien Vet 2008; 31(1-2):15-19.
  24. Morar A, Sala C, Imre K. Occurrence and antimicrobial susceptibility of Salmonella isolates recovered from the pig slaughter process in Romania. J Infect Dev Ctries 2015; 9(1):99-104. https://doi.org/10.3855/jidc.5236
  25. Schmidt JW, Brichta-Harhay DM, Kalchayanand N, Bosilevac JM, Shackelford SD, Wheeler TL et al. Prevalence, Enumeration, Serotypes, and Antimicrobial Resistance Phenotypes of Salmonella enterica Isolates from Carcasses at Two Large United States Pork Processing Plants Appl Environ Microb 2012; 78(8):2716-2726.
  26. Afema JA, Byarugaba DK, Shah DH, Atukwase E, Nambi M, Sischo WM. Potential Sources and Transmission of Salmonella and Antimicrobial Resistance in Kampala, Uganda. Plos One 2016; 11(3):e0152130. https://doi.org/10.1371/journal.pone.0152130
  27. Osman KM, Marouf SH, Zolnikov TR, AlAtfeehy N. Isolation and characterization of Salmonella enterica in day-old ducklings in Egypt. Pathog Glob Health 2014; 108(1):37-48. https://doi.org/10.1179/2047773213Y.0000000118
  28. Stipetic K, Chang Y-C, Peters K, Salem A, Doiphode SH, McDonough PL et al. The risk of carriage of Salmonella spp. and Listeria monocytogenes in food animals in dynamic populations. Vet Med Sci 2016; 2(4):246–254 https://doi.org/10.1002/vms3.39
  29. Cui M, Xie M, Qu Z, Zhao S, Wang J, Wang Y et al. Prevalence and antimicrobialr esistance of Salmonella isolated from an integrated broiler chicken supply chain in Qingdao, China. Food Cont 2016; 62:270-276. https://doi.org/10.1016/j.foodcont.2015.10.036
  30. Correia-Gomes C, Mendonça D, Veira-Pinto M, Niza-Ribeiro J. Risk factors for Salmonella sp. in pig lymph nodes in Portuguese abattoirs. Revue Méd Vét, 2013; 164(4):212-218.
  31. Wang B, Wesley IV, McKean JD, O'Connor AM. Sub-iliac lymph nodes at slaughter lack ability to predict Salmonella enterica prevalence for swine farms. Foodborne Pathog Dis 2010; 7(7):795-800. https://doi.org/10.1089/fpd.2009.0459
  32. Bird P, Flannery J, Crowley E, Agin JR, Goins D, Monteroso L. Evaluation of the 3M™ Molecular Detection Assay (MDA) 2 - Salmonella for the Detection of Salmonella spp. in Select Foods and Environmental Surfaces: Collaborative Study, First Action 2016.01. J AOAC Int 2016; 99(4):980-997. https://doi.org/10.5740/jaoacint.16-0085

Sistema OJS 3.4.0.3 - Metabiblioteca |