In vitro production of gas methane by tropical grasses

Produccion in vitro de gas metano por gramineas forrajeras tropicales

Objetives. Estimate the production of methane (CH₄) by tropical grasses fermented in vitro. Materials and methods. A sample of 20 g dry matter of Cynodon nlemfuensis, Hyparrhenia rufa, Megathyrsus maximus and Digitaria swazilandesis plus 200 ml of culture medium were plated in triplicate flasks sterile stainless steel with CO₂ flux, inoculated with 20 ml of ruminal fluid bovine, incubated at 38 °C for 24, 48, 72 and 96 h. Total production of gas, CH₄, volatile fatty acids, and pH were evaluated in a completely randomized design with three replicates per treatment and comparison of means with Tukey; the concentration of total and cellulolytic bacteria were analyzed with the Kruskal-Wallis, and the GLM procedure independent data Wilcoxon rank. Results. H. rufa and D. swazilandensis both had the lowest total gas production (p<0.05), while D. swazilandesis had lower production of CH₄, increased production of propionic acid (p<0.05) and lower pH 96 hours of incubation (p<0.05). D. swazilandesis showed greater efficiency in energy production due to reduced production of CH₄ and increased propionate production. The concentration of total bacteria was similar between treatments (p>0.05), while the concentration of cellulolytic bacteria was lower in C. nlemfuensis y D. swazilandesis when 96 of incubation (p<0.05). Conclusions. The Digitaria swazilandesis, showed favorable conditions to have lower total methane and total gas production.

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