Assessment of homeopathic medicines on survival and antioxidant response in white shrimp Litopenaeus vannamei

Evaluación de medicamentos homeopáticos en la supervivencia y respuesta antioxidante del camarón blanco Litopenaeus vannamei infectado con Vibrio parahaemolyticus

Published 2018-09-01

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Mazón-Suástegui, J., García-Bernal, M., Avilés-Quevedo, M. A., Campa-Córdova, Ángel, Salas-Leiva, J., & Abasolo-Pacheco, F. (2018). Assessment of homeopathic medicines on survival and antioxidant response in white shrimp Litopenaeus vannamei. Journal MVZ Cordoba, 23(3), 6850-6859. https://doi.org/10.21897/rmvz.1373
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https://doi.org/10.21897/rmvz.1373
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José Mazón-Suástegui
Centro de Investigaciones Biológicas del Noroeste S.C., La Paz, Baja California Sur, México.
http://orcid.org/0000-0003-4074-1180

Milagro García-Bernal
Centro de Bioactivos Químicos, Universidad Central de Las Villas, Santa Clara, Villa Clara, Cuba.
María Araceli Avilés-Quevedo
Instituto Nacional de Pesca (INAPESCA), La Paz, Baja California Sur, México. Investigador jubilado.
http://orcid.org/0000-0001-8647-751X

Ángel Campa-Córdova
Centro de Investigaciones Biológicas del Noroeste S.C., La Paz, Baja California Sur, México.
http://orcid.org/0000-0003-0099-3324

Joan Salas-Leiva
Centro de Investigaciones Biológicas del Noroeste S.C., La Paz, Baja California Sur, México.
Fernando Abasolo-Pacheco
Universidad Técnica Estatal de Quevedo, Facultad de Ciencias Agrarias, Quevedo, Ecuador
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Objective. Evaluating the effect of homeopathic medicines on survival and activity of the superoxide dismutase (SOD) enzyme in white shrimp Litopenaeus vannamei subjected to infection with the pathogenic Vibrio parahaemolyticus. Materials and methods. The average lethal dosage (LD50) was determined for the pathogen strain in L. vannamei juveniles under immersion (Imm), injection (Inj) and incision + immersion (Inc+Imm) methods.Four treatments were applied: (1) Mix CIB®-HOM Heel-Mix (TH1), constituted by equal v/v ratio, of Cyme-Heel®, Gal-Heel®, Hepa-Heel®, Mucs-Heel® and Chol-Heel®; (2) Mix CIB®-HOM Pav-Mix (TH2), constituted by the same v/v ratio of Passiflora incarnata, Valeriana officinalis, Zincum valerianicum and Ignatia amara (Similia®); (3) Heel-Mix/Pav-Mix (TH3) consisting of a 1:1 v/v combination of the TH1 and TH2 treatments, and (4) ViT-Mix (TH4), constituted by Vidatox®, and a control (not treated/infected). Results. While applying the methods Imm, Inj and Inc+Imm, LD50 was 0.9 x 106; 0.6 x 106 and 0.5 x 106 UFC.mL-1, respectively. At the end of the challenge, the groups treated with TH2, TH3 and TH4 had a greater survival rate to that of the control group (p<0.05). Moreover, these two last treatments showed a greater SOD activity with respect to the control group (p<0.05). Conclusions. The homeopathic treatments (TH3 and TH4) increased survival and SOD activity in L. vannamei juveniles challenged with V. parahaemolyticus, which suggests that the homeopathic treatments employed had the potential as an alternative for the control of V. parahaemolyticus and its associated diseases, including the early mortality syndrome in shrimp farming.

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