Assessment of homeopathic medicines on survival and antioxidant response in white shrimp Litopenaeus vannamei

Evaluación de medicamentos homeopáticos en la supervivencia y respuesta antioxidante del camarón blanco Litopenaeus vannamei infectado con Vibrio parahaemolyticus

Published 2018-09-01

Open | Download
PDF (Spanish) FLIP EPUB (Spanish) application/xml XML (Spanish)
How to Cite
Mazón-Suástegui, J., García-Bernal, M., Avilés-Quevedo, M. A., Campa-Córdova, Ángel, Salas-Leiva, J., & Abasolo-Pacheco, F. (2018). Assessment of homeopathic medicines on survival and antioxidant response in white shrimp Litopenaeus vannamei. Journal MVZ Cordoba, 23(3), 6850-6859. https://doi.org/10.21897/rmvz.1373
doi

https://doi.org/10.21897/rmvz.1373
Dimensions
PlumX
José Mazón-Suástegui
Centro de Investigaciones Biológicas del Noroeste S.C., La Paz, Baja California Sur, México.
http://orcid.org/0000-0003-4074-1180

Milagro García-Bernal
Centro de Bioactivos Químicos, Universidad Central de Las Villas, Santa Clara, Villa Clara, Cuba.
María Araceli Avilés-Quevedo
Instituto Nacional de Pesca (INAPESCA), La Paz, Baja California Sur, México. Investigador jubilado.
http://orcid.org/0000-0001-8647-751X

Ángel Campa-Córdova
Centro de Investigaciones Biológicas del Noroeste S.C., La Paz, Baja California Sur, México.
http://orcid.org/0000-0003-0099-3324

Joan Salas-Leiva
Centro de Investigaciones Biológicas del Noroeste S.C., La Paz, Baja California Sur, México.
Fernando Abasolo-Pacheco
Universidad Técnica Estatal de Quevedo, Facultad de Ciencias Agrarias, Quevedo, Ecuador
Perfil Google Scholar
Show authors biography
Objective. Evaluating the effect of homeopathic medicines on survival and activity of the superoxide dismutase (SOD) enzyme in white shrimp Litopenaeus vannamei subjected to infection with the pathogenic Vibrio parahaemolyticus. Materials and methods. The average lethal dosage (LD50) was determined for the pathogen strain in L. vannamei juveniles under immersion (Imm), injection (Inj) and incision + immersion (Inc+Imm) methods.Four treatments were applied: (1) Mix CIB®-HOM Heel-Mix (TH1), constituted by equal v/v ratio, of Cyme-Heel®, Gal-Heel®, Hepa-Heel®, Mucs-Heel® and Chol-Heel®; (2) Mix CIB®-HOM Pav-Mix (TH2), constituted by the same v/v ratio of Passiflora incarnata, Valeriana officinalis, Zincum valerianicum and Ignatia amara (Similia®); (3) Heel-Mix/Pav-Mix (TH3) consisting of a 1:1 v/v combination of the TH1 and TH2 treatments, and (4) ViT-Mix (TH4), constituted by Vidatox®, and a control (not treated/infected). Results. While applying the methods Imm, Inj and Inc+Imm, LD50 was 0.9 x 106; 0.6 x 106 and 0.5 x 106 UFC.mL-1, respectively. At the end of the challenge, the groups treated with TH2, TH3 and TH4 had a greater survival rate to that of the control group (p<0.05). Moreover, these two last treatments showed a greater SOD activity with respect to the control group (p<0.05). Conclusions. The homeopathic treatments (TH3 and TH4) increased survival and SOD activity in L. vannamei juveniles challenged with V. parahaemolyticus, which suggests that the homeopathic treatments employed had the potential as an alternative for the control of V. parahaemolyticus and its associated diseases, including the early mortality syndrome in shrimp farming.

Article visits 2921 | PDF visits

Downloads

Download data is not yet available.
  1. Valdez G, Díaz F, Re AD, Sierra E. Efecto de la salinidad sobre la fisiología energética del camarón blanco Litopenaeus vannamei (Boone). Hidrobiológica 2008; 18(2):105-115.
  3. Soto-Rodriguez SA, Gomez-Gil B, Lozano-Olvera R, Betancourt-Lozano M, Morales-Covarrubias MS 2015. Field and experimental evidence of Vibrio parahaemolyticus as the causative agent of acute hepatopancreatic necrosis disease (AHPND) of cultured shrimp (Litopenaeus vannamei) in northwestern Mexico. Appl Environ Microbiol. 81(5): 1689-1699. https://doi.org/10.1128/AEM.03610-14
  5. Schryver PD, Defoirdt T, Sorgeloos P. Early mortality syndrome outbreaks: a microbial management issue in shrimp farming? PLoS Pathog 2014; 10(4): e1003919 https://doi.org/10.1371/journal.ppat.1003919
  7. Newaj-Fyzul A, Austin B. Probiotics, immunostimulants, plant products and oral vaccines, and their role as feed supplements in the control of bacterial fish diseases. J Fish Dis 2015; 38(11):937-955. https://doi.org/10.1111/jfd.12313
  9. Lakshmi B, Viswanath B, Sai Gopal DVR. Probiotics as antiviral agents in shrimp aquaculture. J Pathog. 2013; Article ID 424123.
  11. Standen BT, Rawling MD, Davies SJ, Castex M, Foey A, Gioacchini G. Probiotic Pediococcus acidilactici modulates both localised intestinal- and peripheral-immunity in tilapia (Oreochromis niloticus). Fish Shellfish Immun 2013; 35(4):1097-1104. https://doi.org/10.1016/j.fsi.2013.07.018
  13. Ortiz-Cornejo NL, Tovar-Ramírez D, Abasolo-Pacheco F, Mazón-Suástegui JM. Homeopatía, una alternativa para la acuicultura. Rev Med Homeopat 2017; 10(1):18-24. https://doi.org/10.1016/j.homeo.2017.04.006
  15. Khuda-Bukhsh AR, Pathak S. Homeopathic drug discovery: theory update and methodological aspect. Expert Opinion on Drug Discovery 2008; 3(8):979-990. https://doi.org/10.1517/17460441.3.8.979
  17. Vockeroth WG. Veterinary homeopathy: an overview. The Can Vet J 1999; 40:592.
  19. Siena CE, Natali MRM, Braccini GL, Oliveira AC, Ribeiro RP, Vargas L. Effect of core homeopathic homeopatila 100® in productive efficiency of fingerlings reverted from Nile tilapia (Oreochromis niloticus). Semina: Ciênc Agrár 2010; 31(4):985–994. https://doi.org/10.5433/1679-0359.2010v31n4p985
  21. Luis-Villase-or IE, Voltolina D, Gomez-Gil B, Ascencio F, Campa-Córdova ÁI, Audelo-Naranjo JM, Zamudio-Armenta OO. Probiotic modulation of the gut bacterial community of juvenile Litopenaeus vannamei challenged with Vibrio parahaemolyticus CAIM 170. Lat Am J of Aquat Res 2015; 43(4):766-775.
  23. Takahashi Y, Itami T, Kondo M, Maeda M, Fujii R, Tomonaga S, Supamattaya K., Boonayaratpalin S. Electron microscopic evidence of bacilliform virus infection in kuruma shrimp (Penaeus japonicus). Fish Pathology 1994; 29:121-125. https://doi.org/10.3147/jsfp.29.121
  25. Roque A, Gómez-Gil B, Guerra Flores AL. Standardization of three techniques for experimental Vibrio infections in the marine shrimp Penaeus vannamei. In Flegel TW (ed) Advances in shrimp biotechnology. National Center for Genetic Engineering and Biotechnology, Bangkok. 1998.
  27. Finney DJ. Probit analysis: a statistical treatment of the sigmoid response curve. 2nd edn. Cambridge University Press; Cambridge: 1952.
  29. Dudonné S, Vitrac X, Coutière P, Woillez M, Mérillon JM. Comparative study of antioxidant properties and total phenolic content of 30 plant extracts of industrial interest using DPPH, ABTS, FRAP, SOD, and ORAC assays. J Agric Food Chem 2009; 57(5):1768-1774. https://doi.org/10.1021/jf803011r
  31. Mazón-Suástegui JM, García-Bernal M, Saucedo PE, Campa-Córdova Á, Abasolo-Pacheco F. Homeopathy outperforms antibiotics treatment in juvenile scallop Argopecten ventricosus: effects on growth, survival, and immune response. Homeopathy 2017; 106(1):18-26. https://doi.org/10.1016/j.homp.2016.12.002
  33. Merlini LS, Vargas L, Piau R, Ribeiro PR, Merlini NB. Effects of a homeopathic complex on the performance and cortisol levels in Nile tilapia (Oreochromis niloticus). Homeopathy 2014; 103:139–142. https://doi.org/10.1016/j.homp.2013.08.005
  35. Gómez B, Roque A, Guerra AL. (). Enfermedades Infecciosas más comunes en la camaronicultura en México y el impacto del uso de antimicrobianos. Instituto de Ciencias del Mar y Limnología; México: 2001.
  37. Saulnier D, Haffner P, Goarant C, Levy P, Ansquer D. Experimental infection models for shrimp vibriosis studies: a review. Aquaculture 2000; 191:133-144. https://doi.org/10.1016/S0044-8486(00)00423-3
  39. Aguirre-Guzmán G, Sánchez-Martínez JG, Pérez-Casta-eda R, Palacios-Monzón A, Trujillo-Rodríguez T, De La Cruz-Hernández NI. Pathogenicity and Infection Route of Vibrio parahaemolyticus in American White Shrimp, Litopenaeus vannamei. J World Aquac Soc 2010; 41: 464–470. https://doi.org/10.1111/j.1749-7345.2010.00388.x
  41. Lemaitre B, Hoffmann J. The host defense of Drosophila melanogaster. Annu Rev Immunol 2007; 25:697-743. https://doi.org/10.1146/annurev.immunol.25.022106.141615
  43. Fridovich I. Oxygen toxicity: a radical explanation. J Exp Biol 1998; 201:1203-1209.
  45. Franco, R., Martín, L., Arenal, A., Santiesteban, D., Sotolongo, J., Cabrera, H., Castillo, N. M. Evaluation of two probiotics used during farm production of white shrimp Litopenaeus vannamei (Crustacea: Decapoda). Aquac Res 2017; 48(4):1936-1950. https://doi.org/10.1111/are.13031
  47. Liu, G., Zhu, S., Liu, D., Guo, X., Ye, Z. Effects of stocking density of the white shrimp Litopenaeus vannamei (Boone) on immunities, antioxidant status, and resistance against Vibrio harveyi in a biofloc system. Fish Shellfish Immun 2017; 67:19-26. https://doi.org/10.1016/j.fsi.2017.05.038
  49. Díaz-García A, Morier-Díaz L, Frión-Herrera Y, Rodríguez-Sánchez H, Caballero-Lorenzo Y, Mendoza-Llanes D, Riquenes-Garlobo Y, Fraga-Castro JA. In vitro anticancer effect of venom from Cuban scorpion Rhopalurus junceus against a panel of human cancer cell lines. J Venom Res 2013; 4:5-12.
  51. Díaz A, Morier L, Rodríguez H, Caballero Y. Citotoxicidad del veneno del escorpión cubano Rhopalurus junceus y sus fracciones sobre líneas celulares tumorales humanas. LABIOFAM 2010; 1:12-18.
  53. Hernández Betancourt O, Casado Hernández I, Iglesias Huerta E, Ramírez Labrada A, Del Risco Ramos J, Rodríguez Pargas A. Evaluación de la toxicidad in vitro del veneno del alacrán Rhopalurus junceus a través de un ensayo celular. Rev Cubana de Invest Bioméd 2009; 28(1):1-11.
  55. Akazawa N, Eguchi M. Environmental trigger for EMS/ AHPNS identified in Agrobest shrimp ponds. Glob Aquacult Advocate 2013; 4:16-17.
  57. Hoanh DTH, Phu TQ, Phuong NT, Tuan PA. Ongoing Vietnam studies find Vibrio with phage transmits EMS/AHPNS. Glob Aquacult Advocate 2013; 4:22-23.

Information

SCImago Journal & Country Rank

0.6
2020CiteScore
 
 
28th percentile
Powered by  Scopus
Redib Widget
Tutorials

Autors:

How send article?

Evaluator pair

How to evaluate an article?

Keywords

QR code

Current Issue

Perfil de Google Scholar

Most read in the last 30 days

Sistema OJS 3.4.0.3 - Metabiblioteca |