Microbiota of Vibrio sp. in the hepatopancreas of cultured white pacific shrimp (Litopenaeus vannamei)
Microbiota of Vibrio sp. in the hepatopancreas of cultured white pacific shrimp (Litopenaeus vannamei)
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ABSTRACT
Objective. The present study aimed to investigate the presence of vibrios in the hepatopancreas of cultured shrimp. Materials and methods. Vibrios from the hepatopancreas of fifteen samples of five specimens each, of apparently healthy Pacific white shrimp (Litopenaeus vannamei) were isolated, identified and quantified. Results. The vibrio density ranged from 430 to 2,400 MPN g-1 (rs MPN cm-1=-0.114; rs MPN g-1 = 0.211). Thirty isolations were obtained, most of which belonged to the species V. cholerae (n=11) and V. parahaemolyticus (n=7). Conclusions. The outcomes of the present study suggest that, even in the absence of symptoms of vibriosis, the microbiota of the hepatopancreas of cultured shrimp may include sucrose positive and negative vibrios.
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- Radjasa OK, Urakawa H, Kita-Tsukamoto K , Ohwad a K . Cha ra c t e ri z a tion o f psychrotrophic bacteria in the surface and deep-sea waters from the Northwestern Pacific Ocean based on 16S ribosomal DNA analysis. Mar Biotechnol 2001; 3:454-462.
- http://dx.doi.org/10.1007/s10126-001-0050-1
- Kannapiran E, Ravindran J, Chandrasekar R, Kalaiarasi A. Studies on luminous, Vibrio harveyi associated with shrimp culture system rearing Penaeus monodon. J Environ Biol 2009; 30:791-795.
- Sizemore RK, Colwell RR, Tubiash HS, Lovelace TE. Bacterial flora of the hemolymph of the blue crab Callinectes sapidus: numerical taxonomy. Appl Microbiol 1975; 29:393-399.
- Gomez-Gil B, Tron-Mayén L , Roque A, Turnbull J F, Inglis V, Guerra-Flores AL. Species of Vibrio isolated from hepatopancreas, haemolymph and digestive tract of a population of healthy juvenile Penaeus vannamei. Aquaculture 1998; 163:1-9.
- http://dx.doi.org/10.1016/S0044-8486(98)00162-8
- Sawabe T, Setoguchi N, Inoue S, Tanaka R, Ootsubo M, Yoshimizu M et al. Acetic acid production of Vibrio halioticoli from alginate: a possible role for establishment of abalone – V. halioticoli association. Aquaculture 2003; 219:671-679.
- http://dx.doi.org/10.1016/S0044-8486(02)00618-X
- Goarant C, Merien F, Berthe F, Mermoud I, Perolat P. Arbitrarily primed PCR to type Vibrio spp. pathogenic for shrimp. Appl Environ Microbiol 1999; 65:1145-1151.
- Sung HH, Hsu SF, Chen CK, Ting YY, Chao W-L. Relationships between disease outbreak in cultured tiger shrimp (Penaeus monodon) and the composition of Vibrio communities in pond water and shrimp hepatopancreas during cultivation. Aquaculture 2001; 192:101-110.
- http://dx.doi.org/10.1016/S0044-8486(00)00458-0
- Austin B, Zhang XH. Vibrio harveyi: a significant pathogen of marine vertebrates and invertebrates. Lett Appl Microbiol 2006; 43:119-124.
- http://dx.doi.org/10.1111/j.1472-765X.2006.01989.x
- Soto Rodriguez SA, Gomez-Gil B, Lozano R, Roque A. Density of vibrios in hemolymph and hepatopancreas of diseased pacific white shrimp, Litopenaeus vannamei, from Northwestern Mexico. J World Aquac Soc 2006; 41:76-83.
- http://dx.doi.org/10.1111/j.1749-7345.2009.00335.x
- Kaysner CA, DePaola Jr A. Vibrio. In: Food and Drug Administration – FDA. Bacteriological Analytical Manual. 2004. [Accessed on: 10 Julio 2010] . URL Available at: http://www.fda.gov/Food/ ScienceResearch/LaboratoryMethods/ BacteriologicalAnalyticalManualBAM/ UCM070830#authors.
- Blodgett R. Appendix 2: most probable numb e r f r om s e ri al dilu ti on s . In : Food and Drug Administration – FDA. Bacteriological Analytical Manual. 2006. [Accessed on: 10 Julio 2010] . URL Available at: http://www.fda.gov/Food/ Science Research/LaboratoryMethods/ BacteriologicalAnalyticalManualBAM/ ucm109656.htm#authors.
- Noguerola I, Blanch AR. Identification of Vibrio spp. with a set of dichotomous keys. J Appl Microbiol 2008; 105:175-185.
- http://dx.doi.org/10.1111/j.1365-2672.2008.03730.x
- Lea-o EM, Lavilla-Pitogo CR, Paner MG. Bacterial flora in the hepatopancreas of pond-reared Penaeus monodon juveniles with luminous vibriosis. Aquacult 1998; 164:367-374.
- http://dx.doi.org/10.1016/S0044-8486(98)00201-4
- Swanson KMJ, Petran RL, Hanlin JH. Culture methods for enumeration of microorganisms. In: Compendium of methods for the microbiological examination of foods. Washington DC: American Public Health Association. 2001.
- http://dx.doi.org/10.2105/9780875531755ch06
- Suzita R, Abdulamir AS, Bakar FA, Son R. A mini review: cholerae outbreak via shellfish. Am J Infect Dis 2009; 5:40-47.
- http://dx.doi.org/10.3844/ajidsp.2009.40.47
- Sung HH, Li HC, Tsai FM, Ting MM, Chao W-L. Changes in the composition of Vibrio communities in pond water during tiger shrimp (Penaeus monodon) cultivation and in the hepatopancreas of healthy and diseased shrimp. J Exp Mar Biol Ecol 1999; 236:261-271.
- http://dx.doi.org/10.1016/S0022-0981(98)00206-8
- Haldar S, Chatter jee S, Asakura M, Vijayakumaran M, Yamasaki S. Isolation of Vibrio parahaemolyticus and Vibrio cholerae (non-O1 and 139) from moribund shrimp (Penaeus monodon) and experimental challenge study against post larvae and juveniles. Ann Microbiol 2007; 57:55-60.
- http://dx.doi.org/10.1007/BF03175050
- Morris Jr JG. Cholera and other types of vibriosis: a story of human pandemics and oysters on the half shell. Clin Infect Dis 2003; 37:272–280.
- http://dx.doi.org/10.1086/375600
- Martin GG, Rubin N, Swanson E. Vibrio parahaemolyticus and V. harveyi cause detachment of the epithelium from the midgut trunk of the penaeid shrimp Sicyonia ingentis. Dis Aquat Org 2004; 60:21-29.
- http://dx.doi.org/10.3354/dao060021
- Thompson FL, Li Y, Gomez-Gil B, Thompson CC, Hoste B, Vandemeulebroecke K, Rupp GS et al. Vibrio neptunius sp. nov., Vibrio brasiliensis sp. nov. and Vibrio xuii sp. nov., isolated from the marine aquaculture environment (bivalves, fish, rotifers and shrimps). Int J Syst Evol Microbiol 2003; 53:245-252.
- http://dx.doi.org/10.1099/ijs.0.02447-0
- Ben-Haim Y, Thompson FL, Thompson CC, Cnockaert MC, Hoste B, Swings J et al. Vibrio coralliilyticus sp. nov., a temperaturedependent pathogen of the coral Pocillopora damicornis. Int J Syst Evol Microbiol 2003; 53:309-315.
- http://dx.doi.org/10.1099/ijs.0.02402-0