Analysis of mdr1-1Δ mutation of MDR1 gene in the “Cimarron Uruguayo” dog

Analysis of mdr1-1Δ mutation of MDR1 gene in the “Cimarron Uruguayo” dog

ABSTRACT

Objective. The aim of this paper is to analyze the frequency of the mdr1-1D mutation of the MDR1 gene in a dog sample of the Uruguayan Cimarron breed with the objective of increasing the knowledge of this breed’s genome. Materials and methods. Thirty-six animals of this breed were analyzed. The MDR1 gene region, which includes the location where the mutation would be present, was amplified by PCR. Results. The mutation was not detected in any of the analyzed Uruguayan Cimarron. Conclusions. The lack of described ivermectin intoxication cases in veterinary clinic in this breed is explained by the lack of the mutation object of this study. The sequence studied in Cimarron dogs is kept compared to other breeds, except Collies and related breeds (Border Collie, Bearded Collie, Old English sheepdog).

1. Gagliardi R. Estudios genéticos en caninos de raza Cimarrón Uruguayo (Canis familiaris). [Tesis de Maestría]. Montevideo: Universidad de la República, Programa de Desarrollo de las Ciencias Básicas (PEDEClBA); 2009.
3. KCU. Cimarrón Uruguayo. [En linea]. Kennel Club Uruguayo. [Consultado noviembre de 2011] URL Disponible en: http://www.kcu.com.uy.
5. Geyer J, Döring B, Godoy JR, Leidolf R, Moritz A, Petzinger E. Frequency of the nt230 (del4) MDR1 mutation in Collies and related dog breeds in Germany. J Vet Pharmacol Therap 2005; 28, 545–551. http://dx.doi.org/10.1111/j.1365-2885.2005.00692.x
7. Neff MW, Robertson KR, Wong AK, Safra N, Broman KW, Slatkin M, et al. Breed distribution and history of canine mdr1-1D, a pharmacogenetic mutation that marks the emergence of breeds from the collie lineage. PNAS 2004; 101(32):11725-30. URL Disponible en: http://www.pnas.org/content/101/32/11725.full
9. Lifschitz A, Virkel G, Imperiale F, Pis A, Lanusse C. Fármacos endectocidas: avermectinas y milbemicinas. En: Botana LM, Landoni F, Martín-Jiménez T. Farmacología y Terapéutica Veterinaria. Mc Graw-Hill. Interamericana. 2002.
11. Mealey K, Bentjen S, Gay J, Cantor G. Ivermectin sensitivity in collies is associated with a deletion mutation of the mdr1 gene. Pharmacogenetics 2001; 11(8):727-33. http://dx.doi.org/10.1097/00008571-200111000-00012
13. Ballent M, Lifschitz A, Virkel G, Lanusse C. Implicancias fisio-farmacológicas de la glicoproteína-P en animales domésticos. Analecta Vet 2005; 25(2):36-47.
15. Llambí S. Estudios citogenéticos-moleculares de la fragilidad del cromosoma sexual X y enfermedades hereditarias monogénicas en bovinos de la raza Holando Uruguayo (Bos taurus). [Tesis Doctoral]. Espa-a: Universidad de Zaragoza; 2002.
17. Hall TA. BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symp Ser 1999; 41:95-8.
19. Kawabata A, Momio Y, Inoue-Murayama M, Iwasaki T. Canine mdr1 Gene Mutation in Japan. J Vet Sci 2005; 67(11):1103-07. http://dx.doi.org/10.1292/jvms.67.1103