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In vitro evaluation of five ixodicides against Rhipicephalus microplus in Catacamas, Olancho, Honduras

Evaluación in vitro de cinco ixodicidas contra Rhipicephalus microplus en Catacamas, Olancho, Honduras



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Lepe López, M. A., & Brizo-Murillo, J. M. . (2022). In vitro evaluation of five ixodicides against Rhipicephalus microplus in Catacamas, Olancho, Honduras. Journal MVZ Cordoba, 27(2), e2463. https://doi.org/10.21897/rmvz.2463

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Manuel Antonio Lepe López
Josselyn Maryeri Brizo-Murillo

Manuel Antonio Lepe López,

Universidad de San Carlos de Guatemala, Facultad de Medicina Veterinaria y Zootecnia, Escuela de Estudios de Postgrado, Ciudad de Guatemala, Guatemala.
Universidad Andres Bello, Life Sciences Faculty, Sustainability Research Centre, Santiago, Chile.


Objective. To evaluate the in vitro efficacy of five commercial products used in the site with the highest cattle production in Honduras. Materials and methods. Ticks were collected in four cattle farms in Catacamas (14 ° 50 'N, 85 ° 53' S), considering a quantity of 300 bovines. Approximately 1,000 R. microplus ticks were collected to implement a control group and five comparison groups, each group included two replicates with 50 individuals. The immersion method was performed with each of the five commercial products and the following rates were estimated: mortality, oviposition, hatching, reproductive efficiency and product efficacy as proposed by Drummond et al., 1973. Results. Four of the commercial products with the following active ingredients (Product 1: cypermethrin, chlorpyrifos, piperonyl butoxide. Product 2: cypermethrin, ethion, piperonyl butoxide. Product 3: amitraz. Product 4: coumaphos), obtained an efficacy > 90 %, standing out the chemical families of amidines, organophosphates and the combination of organophosphates with pyrethroids for the control of R. microplus in the town of Catacamas, Honduras. On the other hand, a product (Product 5: cypermethrin), has an efficiency of 68%. This product of the pyrethroid family shows efficacy problems with mortality of 33% and the surviving individuals have a reproductive rate of 30%. Conclusions. It is necessary to modify the use of cypermethrin in Catacamas, Honduras, incorporating a comprehensive control of parasites to reduce the possibility of antiparasitic resistance of R. microplus.


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