Asociación entre receptores de leptina en testículo, niveles de leptina y testosterona en terneros púberes

Asociación entre receptores de leptina en testículo, niveles de leptina y testosterona en terneros púberes

Published 2010-09-01

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Ruiz, Z., & Olivera, M. (2010). Asociación entre receptores de leptina en testículo, niveles de leptina y testosterona en terneros púberes. Journal MVZ Cordoba, 15(3). https://doi.org/10.21897/rmvz.307
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https://doi.org/10.21897/rmvz.307
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Z.Tatiana Ruiz
Martha Olivera
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Z.Tatiana Ruiz

Universidad de Antioquia, Facultad de Ciencias Agrarias, Grupo de Investigación Biogénesis, Robledo, Medellín

Martha Olivera

Universidad de Antioquia, Facultad de Ciencias Agrarias, Grupo de Investigación Biogénesis, Robledo, Medellín
Objetivos. Describir la presencia de receptores de leptina (OBR) en el testículo en la llegada de la pubertad en terneros y estudiar la posible asociación entre la expresión de éstos y los niveles de leptina (L) y de testosterona. Materiales y métodos. Se utilizaron 6 terneros Holstein x Cebú a los cuales se les midió quincenalmente y durante 7 meses concentraciones de testosterona y de L por RIA. Fueron castrados unilateralmente según períodos peripuberales para estudiar por RT-PCR la expresión de isoformas del receptor de L. El análisis estadístico se realizó con el programa Statistica®. Resultados. La testosterona presentó niveles desde menores a 1ng/mL a los 6 y 6.5 meses de edad, hasta concentraciones de 5.3 ng/ml a los 12.5 meses antes de la llegada a pubertad, momento en el cual los animales tuvieron niveles de 4.01±1.8 ng/ml. Las concentraciones de L variaron entre 0.61 y 0.98 ng/ml, con una concentración en pubertad de 0.91±0.05 ng/ml. Dos isoformas, OBRi y OBRb, fueron encontradas y se correlacionaron significativamente entre ellas en la pubertad. Se hallaron correlaciones negativas entre OBRi-OBRb y niveles de testosterona y de L (p=0.08). Los niveles de testosterona y de L mostraron una correlación directa significativa. Conclusiones. Se sugiere un posible efecto directo de la leptina en gónadas de terneros hasta la llegada a pubertad. La correlación entre las isoformas de OBR y su asociación con las concentraciones de leptina y testosterona también sugiere la acción complementaria o conjunta de ambos receptores OBR en testículos de terneros peripuberales.

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