First approach to molecular epidemiology of bovine tuberculosis in Colombia

First approach to molecular epidemiology of bovine tuberculosis in Colombia

ABSTRACT

Objective. To investigate the presence of Mycobacterium bovis and other Mycobacterium species in samples of cattle and buffalo in Colombia, to start the molecular characterization of M. bovis in the country. Material and methods. 492 samples were collected from herds identified with the presence of infected animals through the PPD, by the Group of Bovine Tuberculosis ICA Colombian Agricultural Institute in eight departments of Colombia. Lymph nodes of head, thorax and abdomen, gross lesions of tissues with tuberculosis, nasal swabs, milk, blood and fresh cheeses were included. Samples were subjected to detection of Mycobacterium bovis and other mycobacteria by conventional microbiological analysis and PCR-6110 and spoligotyping molecular assays. Results. In the samples analyzed especially in lymph nodes, Mycobacterium bovis was demonstrated with genotypes reported and not previously reported in the world, as well as M. tuberculosis in Antioquia, Cundinamarca, Boyacá and Magdalena departments. Conclusions. In Colombia there are at least 7 genotypes of M. bovis that are infected and sick cattle and buffalo from four different departments becoming serious threat to public health.

RESUMEN

Objetivo. Investigar la presencia de Mycobacterium bovis y otras especies de Mycobacterium en muestras de ganado bovino y búfalino en Colombia e iniciar la caracterización molecular de M. bovis en el país. Materiales y métodos. 492 muestras fueron recogidas de hatos identificados con presencia de animales infectados a través de la prueba de PPD, por el Grupo de Tuberculosis Bovina del Instituto Colombiano Agropecuario ICA en ocho departamentos de Colombia. Se incluyeron ganglios linfáticos de cabeza, tórax y abdomen, tejidos con lesiones macroscópicas de tuberculosis, hisopos nasales, leche, sangre y quesos frescos. Las muestras se sometieron a investigación de Mycobacterium bovis y otras micobacterias mediante el análisis microbiológico convencional y pruebas moleculares de PCR-IS6110 y spoligotyping. Resultados. En las muestras analizadas especialmente en los ganglios linfáticos, se evidenció Mycobacterium bovis con genotipos reportados y no reportados previamente en el mundo, así como M. tuberculosis en los departamentos de Antioquia, Cundinamarca, Magdalena y Boyacá. Conclusiones. En Colombia existen al menos 7 genotipos de M. bovis que se encuentran infectando y enfermando a bovinos y bufalinos de cuatro diferentes departamentos del país constituyéndose en seria amenaza para la salud pública.

1. De la Rúa D. Human Mycobacterium bovis infection in the United Kingdom: Incidence, risks, control measures and review of the zoonotic aspects of bovine tuberculosis. Tuberculosis 2006; 86(2):77-109. http://dx.doi.org/10.1016/j.tube.2005.05.002
3. Müller B, Dürr S, Alonso S, Hattendorf J, Laisse C, Parsons S, et al. Zoonotic Mycobacterium bovis– induced tuberculosis in humans. Emerg Infect Dis 2013; 19(6):899-908. http://dx.doi.org/10.3201/eid1906.120543
5. Anaelom NJ, Ikechukwu ON, Sunday EW, Nnaemeka UC. Zoonotic tuberculosis: A review of epidemiology, clinical presentation, prevention and control. J Public Health Epidemiol 2010; 2(6):118-24.
7. Instituto Colombiano Agropecuario ICA. Estadísticas del Programa de Tuberculosis y Brucelosis [en línea] 2014 [Ultimo ingreso Octubre 14 2014]. URL disponible en: http://www.ica.gov.co/getdoc/e3fc207f-c332-4e38-a50d-de61c338ace6/Campana-de-erradicacion.aspx
9. Bennett RM. Farm costs associated with premovement testing for bovine tuberculosis. Vet Rec 2009; 164(3):77-79. http://dx.doi.org/10.1136/vr.164.3.77
11. Schiller I, Oesch B, Vordermeier H.M., Palmer M.V., Harris B.N., Orloski K.A., et al. Bovine Tuberculosis: A review of current and emerging diagnostic techniques in view of their relevance for disease control and eradication. Transbound Emerg Dis 2010; doi:10.1111/j.1865-1682.2010.01148.x http://dx.doi.org/10.1111/j.1865-1682.2010.01148.x
13. Departamento de Planeación Nacional DPN. Consejo Nacional de Política Económica y Social. CONPES 3376. Política sanitaria y de inocuidad para las cadenas de la carne bovina y de la leche. [en línea] 2010 [Ultimo ingreso Octubre 1 2014]. URL disponible en: http://www.ica.gov.co/getattachment/3b31038a-72ba-40f9-a34d-cecd89015890/2010cp3676.aspx.
15. Departamento Administrativo Nacional de Estadística DANE. Encuesta de sacrificio de ganado. [en línea] 2014 [Ultimo ingreso Abril 30 2015]. URL disponible en: http://www.dane.gov.co/index.php/agropecuario-alias/estadisticas-de-sacrificio-de-ganado-esag.
17. Zumárraga MJ, Soutullo A, García MI, Marini R, Abdala A, Tarabla H, et al. Detection of Mycobacterium bovis-infected dairy herds using PCR in bulk tank milk samples. Foodborne Pathog Dis 2012; 9(2):132-137. http://dx.doi.org/10.1089/fpd.2011.0963
19. Medeiros Ldos S, Marassi CD, Figueiredo EE, Lilenbaum W. Potential application of new diagnostic methods for controlling bovine tuberculosis in Brazil. Braz J Microbiol 2010; 41(3):531-41. http://dx.doi.org/10.1590/S1517-83822010005000002
21. Silva MR, Rocha Ada S, da Costa RR, de Alencar AP, de Oliveira VM, Fonseca Júnior AA, et al.Tuberculosis patients co-infected with Mycobacterium bovis and Mycobacterium tuberculosis in an urban area of Brazil. Mem Inst Oswaldo Cruz 2013; 108(3):321–327. http://dx.doi.org/10.1590/s0074-02762013000300010
23. Taylor GM, Worth DR, Palmer S, Jahans K, Hewinson RG. Rapid detection of Mycobacterium bovis DNA in cattle lymph nodes with visible lesions using PCR. BMC Vet Res 2007; 3:12. doi:10.1186/1746-6148-3-12 http://dx.doi.org/10.1186/1746-6148-3-12
25. Miller S, Sweeney S. Mycobacterium bovis (bovine tuberculosis) infection in North American wildlife: current status and opportunities for mitigation of risks of further infection in wildlife populations. Epidemiol Infect 2013; 141(7):1357–1370. http://dx.doi.org/10.1017/S0950268813000976
27. Tadayon K, Mosavari N, Feizabadi M. An epidemiological perspective on bovine tuberculosis spotlighting facts and dilemmas in Iran, a historically zebu-dominant farming country. Iran J Microbiol 2013; 5(1):1-13.
29. Thakur A, Sharma M, Katoch V, Dhar P, Katoch R. Detection of Mycobacterium bovis and Mycobacterium tuberculosis from Cattle: Possible Public Health Relevance. Indian J Microbiol 2012; 52(2):289-291 http://dx.doi.org/10.1007/s12088-011-0200-8
31. Rodwell TC, Kapasi AJ, Moore M, Milian-Suazo F, Harris B, Guerrero LP, et al. Tracing the origins of Mycobacterium bovis tuberculosis in humans in the USA to cattle in Mexico using spoligotyping. Int J Infect Dis 2010; 14(S3):e129-e135. http://dx.doi.org/10.1016/j.ijid.2009.11.037
33. Instituto Colombiano Agropecuario ICA. Sistema de vigilancia epidemiológica del ICA tuberculosis y brucelosis. [en línea] 2014 [Ultimo ingreso Octubre 1 2014]. URL disponible en: http://www.ica.gov.co/Trazabilidad/Normatividad/Files/Resoluciones/ICA/resolucion-185-de-2007.aspx
35. Silva A del P, León CI, Guerrero MI, Neira R, Arias L, Rodríguez G. Avian tuberculosis of zoonotic importance at a zoo on the Bogotá Andean plateau (Sabana), Colombia. Can Vet J 2009; 50(8):841-845.
37. Organización Mundial de Sanidad Animal OIE. Tuberculosis Bovina. Manual de las Pruebas de Diagnóstico y de las Vacunas para los Animales Terrestres. [En línea] 2008. [Último ingreso Octubre 1 2014].URLdisponibleen:http://www.oie.int/es/normas-internacionales/manual-terrestre/acceso-en-linea/.
39. van Soolingen D, De Hass PE, Kremer K. Restriction fragment length polymorphism (RFLP) typing of Mycobacteria. National Institute of Public Health and the Environment. 2th Ed. Bilthoveen; 2010.
41. Kremer K, van Soolingen D, Frothingham R, Haas WH, Hermans PW, Martín C, et al. Comparison of methods based on different molecular epidemiological markers for typing of Mycobacterium tuberculosis complex strains: interlaboratory study of discriminatory power and reproducibility. J Clin Microbiol 1999; 37(8):2607-2618.
43. Kamerbeek J, Schouls L, Kolk A, van Agterveld M, van Soolingen D, Kuijper S, et al. Simultaneous detection and strain differentiation of Mycobacterium tuberculosis for diagnosis and epidemiology. J Clin Microbiol 1997; 35(4):907-914.
45. Brudey K, Driscoll JR, Rigouts L, Prodinger WM, Gori A, Al-Hajoj S, et al. Mycobacterium tuberculosis complex genetic diversity: mining the fourth international spoligotyping database (SpolDB4) for classification, population genetics and epidemiology. BMC Microbiology 2006; 6:23-40 doi:10.1186/1471-2180-6-23 http://dx.doi.org/10.1186/1471-2180-6-23
47. MbDB, M bovis spoligotypes Database. [on line] 2014 [Último ingreso Octubre 30 2014]. URL disponible en: http://www.mbovis.org/spoligodatabase/intro.htm.
49. Allison, P. Logistic Regression, Using the SAS System. SAS OnlineDoc® 9.2. Cary, NC: SAS Institute Inc. 2007.
51. Colombia. Ministerio de la Protección Social. Decreto número 1880 de 2011. Diario Oficial N° 48.085 del 30 de mayo de 2011. URL disponible en: http://www.aladi.org/nsfaladi/normasTecnicas.nsf/09267198f1324b64032574960062343c/543bd5fa59bedc3a032579de00526b80/$FILE/Decreto%20N%C2%B0%201880-2011.pdf
53. Zumárraga MJ, Arriaga C, Barandiaran S, Cobos-Marín L, de Waard J, Estrada-Garcia I, et al. Understanding the relationship between Mycobacterium bovis spoligotypes from cattle in Latin American countries. Res Vet Sci 2013; 94(1):9-21. http://dx.doi.org/10.1016/j.rvsc.2012.07.012
55. de Kantor IN, Ambroggi M, Poggi S, Morcillo N, Da Silva-Telles M, Osório-Ribeiro M, et al. Human Mycobacterium bovis infection in ten Latin American countries. Tuberculosis (Edinb) 2008; 88(4):358-65 http://dx.doi.org/10.1016/j.tube.2007.11.007
57. Hernández-Pando R, Marquina-Castillo B, Barrios-Payán J, Mata-Espinosa D. Use of mouse models to study the variability in virulence associated with specific genotypic lineages of Mycobacterium tuberculosis. Infect Genet Evol. 2012;12(4):725-31. doi: 10.1016/j.meegid.2012.02.013. http://dx.doi.org/10.1016/j.meegid.2012.02.013
59. Gumi B, Schelling E, Berg S, Firdessa R, Erenso G, Mekonnen W, et al. Zoonotic transmission of tuberculosis between pastoralists and their livestock in South-East Ethiopia. Ecohealth 2012; 9(2):139-49. http://dx.doi.org/10.1007/s10393-012-0754-x
61. Ocepek M, Pate M, Zolnir-Dovc M, Poljak M. Transmission of Mycobacterium tuberculosis from human to cattle. J Clin Microbiol 2005; 43(7):3555–3557. http://dx.doi.org/10.1128/JCM.43.7.3555-3557.2005
63. Sanou A, Tarnagda Z, Kanyala E, Zingué D, Nouctara M, Ganame Z, et al. Mycobacterium bovis in Burkina Faso: Epidemiologic and Genetic Links between Human and Cattle Isolates. PLoS Negl Trop Dis 2014; 8(10):e3142.
64. http://dx.doi.org/10.1371/journal.pntd.0003142