Analysis of two populations of the uruguayan canine breed cimarron (Canis familiaris) using rapd markers
Análisis de dos poblaciones de caninos (Canis familiaris) de la raza cimarrón uruguayo utilizando rapds como marcadores moleculares
Show authors biography
Objective. To analyze the genetic variability in two populations of the native canine breed “Uruguayan Cimarron” from different areas of the country. Materials and methods. DNA was isolated from 40 animals of the Cimarron breed. Using PCR, 11 RAPD (random amplified polymorphism of DNA) molecular markers were analyzed. Results. 14.9% of loci were polymorphic. We found an index of shared bands of 0.91, a genetic distance of 0.16 and a Nei’s average diversity index of 0.08. Conclusions. Both populations present a high homogeneity and genetic identity for these markers suggesting that they belong to a common genetic nucleus.
Article visits 710 | PDF visits
Downloads
- Fernández G. Situación de los recursos genéticos domésticos locales del Uruguay. Arch Zootec 2000;49:333-340.
- Silveira C, Fernández G, Barba C. Primeros datos de la caracterización etnológica del perro cimarrón. Arch Zootec 2002: 51:223-228.
- Silveira C, Mernies B, Fernández G, Barba C. Estudio biométrico de una población canina de la raza cimarrón. Arch Zootec 1998; 47:529-532.
- Olivier M, Meehl M, Lust G. Random amplified polymorphic DNA (RAPD) sequences as markers for canine genetic studies. J Hered 1999; 90:78-82.
- https://doi.org/10.1093/jhered/90.1.78
- Rothuizen J, Van Wolferen M. Randomly amplified DNA polymorphisms in dogs are reproducible and display Mendelian transmission. Anim Genet 1994; 25:13-18.
- https://doi.org/10.1111/j.1365-2052.1994.tb00441.x
- https://doi.org/10.1111/j.1365-2052.1994.tb00049.x
- Stepniak E, Zagalska M, Switonski M. Use of RAPD technique in evolution studies of tour species in the family Canidae. J Appl Genet 2002; 43(4):489-499.
- Morera Sanz L, Barba C, Garrido J. Detección de variabilidad en razas caninas autóctonas espa-olas mediante marcadores RAPD. Arch Zootec 2001; 50:379-382.
- Llambí MS, Separovich MJ, Fernández G, Arruga MV. Primeros estudios moleculares en el perro Cimarrón del Uruguay. Veterinaria 2004; 39(155-156):65-68.
- Cheung W, Hubert N, Landry B. A simple and rapid DNA microextracción method for plant, animal, and insect suitable for RAPD and other PCR analyses. PCR Methods Appl 1993; 3:69-70.
- https://doi.org/10.1101/gr.3.1.69
- Linch M. The similarity index and DNA fingerprinting. Mol Biol Evol 1990; 7:478-484.
- Yeh F, Yang R, Boyle T, Ye Z, Mao J. PopGene. Alberta, Canada: University of Alberta and Center for International Forestry Research; 1967.
- Morera Sanz L, de Andrés D, Barbancho J, Garrido J, Barba C. Detección de variabilidad genética por microsatélites en el alano espa-ol. Arch Zootec 1999;48:72-77.
Information
