Purificación de IgY contra la subunidad nr3 del receptor nmda de cerebro de rata

Purificación de IgY contra la subunidad nr3 del receptor nmda de cerebro de rata

Published 2008-01-01

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Méndez, G., Reyes, E., Poutou, R., Quevedo, B., & Lareo, L. (2008). Purificación de IgY contra la subunidad nr3 del receptor nmda de cerebro de rata. Journal MVZ Cordoba, 13(1). https://doi.org/10.21897/rmvz.407
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https://doi.org/10.21897/rmvz.407
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Gina Méndez
Edgar Reyes
Raúl Poutou
Balkys Quevedo
Leonardo Lareo
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Objetivo. Obtener anticuerpos tipo IgY contra péptidos sintéticos de las subunidades NR3A y NR3B del receptor NMDA de ratas, para reconocer y seguir la expresión de estas subunidades en extractos de cerebro de rata de diferentes edades. Materiales y métodos. Se diseñaron dos péptidos empleando los sistemas de la base de datos Entrez y el programa ClustalWPBIL de alineamientos múltiples contra las subunidades NR3A y NR3B del receptor NMDA; una vez sintetizados por el método SSPS-fmoc fueron utilizados para inocular gallinas (Gallus gallus, variedad Hy Line Brown) de 16 semanas de edad; al cabo de 57 días postinoculación se purificó IgY específica y se enfrentaron a extractos de cerebro de rata postnatal y adulta. Resultados. Se detectaron las subunidades NR3A y NR3B y se relacionó su expresión con la edad del animal; siendo mayor la expresión de la subunidad NR3A en extracto de cerebro de rata postnatal. No se encontró diferencia marcada en la expresión de la subunidad NR3B en las edades mencionadas. Conclusiones. Esta es la primera investigación que emplea proteína nativa para el reconocimiento de la subunidad NR3 del receptor NMDA, lo cual muestra la especificidad de los anticuerpos generados y contribuye con el entendimiento de las funciones de este receptor y su relación con la regulación de la memoria espacial

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