Efecto del co-cultivo sobre el desarrollo temprano de embiones bovinos producidos in vitro

Efecto del co-cultivo sobre el desarrollo temprano de embiones bovinos producidos in vitro

Published 2007-07-01

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López C, A., Olivera A, M., Ruiz C, T., & Tarazona M, A. (2007). Efecto del co-cultivo sobre el desarrollo temprano de embiones bovinos producidos in vitro. Journal MVZ Cordoba, 12(2). https://doi.org/10.21897/rmvz.428
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https://doi.org/10.21897/rmvz.428
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Angela López C
Martha Olivera A
Tatiana Ruiz C
Ariel Tarazona M
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Objetivo. Estudiar el efecto del cocultivo con células oviductales sobre el porcentaje de clivaje 48 horas post inseminación (hpi) de embriones bovinos en bajas tensiones de oxígeno. Materiales y métodos. Se recolectaron ovarios de matadero para la extracción de los ovocitos que fueron puestos en medio TCM 199 suplementado con hormonas, se fertilizaron con semen criópreservado y se cocultivaron en medio CR1aa con células de oviducto durante 48 horas. Se evaluó el porcentaje de clivaje total y el porcentaje de clivaje por estadios de 2-4 células y 5-8 células. La viabilidad de las células para el cocultivo se determinó por observación del movimiento ciliar y observación de monocapa. Los tratamientos fueron T1: células de oviducto + oxígeno 20%; T2: células de oviducto + oxígeno 7%; T3 y T4 fueron controles sin células para ambas concentraciones de oxígeno. Resultados. En cuanto al porcentaje de clivaje no hubo diferencia significativa entre los cuatro tratamientos, pero hubo una tendencia a mayor clivaje para los embriones cocultivados con células y 20% oxígeno. Conclusiones. La utilización de altas tasas de oxígeno (20%) en los sistemas de cococultivo con células oviductales tienden a mejorar los porcentajes de clivaje a las 48 hpi.

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