Physiology of acid-base balance in bovines with diarrhea backgrounds from Monteria, Colombia

Physiology of acid-base balance in bovines with diarrhea backgrounds from Monteria, Colombia

ABSTRACT

Objective. Evaluate the acid-base balance (ABB) in bovines with diarrheic backgrounds in four areas of Montería, Colombia. Materials and methods. From a total of 300 pregnant cows, 60 were selected with their newborns. A direct inspection was performed of vital signs on the calves and the ABB indicators were determined using a gasometric method. Data were processed by means of descriptive statistics and the Duncan test was used to differentiate between the averages. The degree of association was established between the ABB indicators in cows and calves by using the Pearson correlation and a comparison of proportions was performed on the indexes of the newborns. Results. Regarding the cows, the ABB indicators were found within the reference values; however, in the calves the pH, pCO2, HCO3-, the anion gap (AG) and the bases excess (BE) varied. A correlation was found between AG, BE and metabolic hydrogen ions (M*H). The AG in cows and calves showed notable differences (p<0.05) among the farms in the study. According to the numeric classification system, the suction reflect indicated a greater percentage of calves in group one. Conclusions. The ABB analyte measurement in cows was similar to the consulted reference; however, in calves some analytes did not coincide. This suggests metabolic acidosis in newborn calves due to the increase of AG and the decrease of BE. Additionally, its correlation with M*H opens the possibility of new proposals to determine ABB in bovines.

RESUMEN

Objetivo. Evaluar el equilibrio ácido-básico (EAB) en bovinos con antecedentes diarreicos de cuatro zonas de Montería, Colombia. Materiales y métodos. De un total de 300 vacas preñadas se seleccionaron 60 vacas y sus recién nacidos. Se realizó una inspección directa de los signos vitales en terneros y los indicadores del EAB se determinaron mediante técnica gasométrica. Los datos se procesaron mediante estadística descriptiva y para la diferencia entre medias se empleó la prueba de Duncan. El grado de asociación se estableció entre los indicadores del EAB en vacas y terneros mediante la correlación de Pearson y una comparación de proporciones se realizó en los índices del recién nacido. Resultados. En las vacas, los indicadores del EAB se encontraron dentro de los valores de referencia; sin embargo, en los terneros varió el pH, pCO2, HCO3-, la brecha aniónica (AG) y el excesode bases (EB). Se encontró correlación entre AG, EB e hidrogeniones metabólicos (H+M). El AG envacas y terneros indicó diferencias notables (p<0.05) entre las fincas de estudio. De acuerdo con elsistema de calificación numérica, el signo reflejo de succión indicó el mayor porcentaje de terneros enel grupo uno. Conclusiones. La medición de los analitos del EAB en vacas fue similar a la referenciaconsultada; sin embargo, en terneros no coincidió con algunos analitos. Se sugiere acidosis metabólicaen los terneros recién nacidos por el aumento del AG y descenso del EB, además, su correlación conlos H+M abre la posibilidad de nuevas propuestas para la determinación del EAB en bovinos.

1. Cho YI1, Yoon KJ. An overview of calf diarrhea - infectious etiology, diagnosis, and intervention. J Vet Sci 2014;15(1):1-17.
2. http://dx.doi.org/10.4142/jvs.2014.15.1.1
4. Lorenz I, Fagan J, More SJ. Calf health from birth to weaning. II. Management of diarrhea in pre-weaned calves. Ir Vet J 2011; 64(1):1-6. http://dx.doi.org/10.1186/2046-0481-64-9
6. Murray CF, Leslie KE. Newborn calf vitality: risk factors, characteristics, assessment, resulting outcomes and strategies for improvement. Vet J 2013; 198(2):322-328.
7. http://dx.doi.org/10.1016/j.tvjl.2013.06.007
9. Baquero-Parrado JR. Diarrea neonatal indiferenciada en terneros: consideraciones sobre su prevención en campo. Vet Zoo Tec 2008; 2(2):59-68.
11. Carrillo R, Visoso P. Equilibrio ácido-base. Conceptos actuales. Rev Asoc Mex Med Crit y Ter Int 2006; 20(4):184-192
13. Hasselbalch KA. Die Berechnung der wasserstoltzahl des blutes ous der freien und gebunden kohlensaure desselben, und die sauerstoltbindung des blutes als function der wassertozahl. Biochem Z 1917; 78:112-144.
15. Kaneko J, Harvey JW, Bruss ML. Clinical biochemistry of domestic animals. 6a ed. Amsterdam: Elsevier Inc; 2008.
17. Oetzel GR. Monitoring and testing dairy herds for metabolic disease. Vet. Clin. North Am., Food Anim Pract 2004; 20(3):651-674. http://dx.doi.org/10.1016/j.cvfa.2004.06.006
19. Singer RB. Haslings AB. An improved clinic method for the estimation of disturbances of the acid-base balance of human blood. Medicine (Baltimore) 1948; 27(2):223-242.
20. http://dx.doi.org/10.1097/00005792-194805000-00003
22. Andersen OS, Engel K, Jorgensen K, Astrup P. A micro method for determination of pH, carbon dioxide tension, base excess and standard bicarbonate in capillary blood. Scand J Clin Lab Invest 1960; 12:172-176. http://dx.doi.org/10.3109/00365516009062419
24. Bookallil MJ. pH of the blood: acid base balance. [en línea]. University of Sydney. 2010. (acceso septiembre de 2013); URL disponible en: http://www.anaesthesia.med.usyd.edu.au/resources/lectures/acidbase_mjb/acidbase.html
26. Emmelt M. Narins RG. CIinical use of the Anión Gap. Medicine (Baltimore) 1977; 56(1):38-54. http://dx.doi.org/10.1097/00005792-197756010-00002
28. Radostits OM, Gay CC, Hinchcliff KW. Veterinary Medicine. A textbook of the diseases of cattle, horses, sheep, pigs, and goats. 10th ed. Philadelphia: Elsevier Inc; 2007.
30. Cruz LE, Diaztagle JJ, Giraldo E, Melo CE, Sprockel JJ. Comparación de diferentes medidas para el abordaje fisiológico del estado ácido-base en pacientes críticos: papel de los hidrogeniones metabólicos. Acta Colombiana de Cuidado Intensivo 2009; 9(2):131-144.
32. Kraut JA, Madias NE. Metabolic acidosis: pathophisiology, diagnosis and management. Nat Rev Nephrol 2010; 6:274-285. doi:10.1038/nrneph.2010.33 http://dx.doi.org/10.1038/nrneph.2010.33
34. Resolución 002341. [en linea]. Bogotá, Colombia: ICA-FEDEGAN. Instituto Colombiano Agropecuario; 2007. URL Disponible en: http://www.slideshare.net/Fedegan/resolucion-2341
36. Mrad de Osorio A. ética en la investigación con modelos animales experimentales. Revista Colombiana de Bioética 2006; 1(1):163-184.
38. Kasari TR, Naylor JM. Further studies on the clinical features and clinicopathological findings of a syndrome of metabolic acidosis with minimal dehydration in neonatal calves. Can J Vet Res 1986; 50(4):502-508.
40. SPSS Inc. SPSS for Windows Version 16.0. Chicago, Illinois, USA: SPSS Inc; 2007.
42. Freitas, MD. Avaliação dos parâmetros clínicos e de patologia clínica em bezerros naturalmente infectados com diarréia neonatal. [Dissertação Mestrado em Medicina Veterinária]. Brasil: Universidade Federal de Minas Gerais, Escola de Veterinária; 2009. URL Disponible en: http://www.bibliotecadigital.ufmg.br/dspace/handle/1843/SSLA-83KKES
44. Herosimczyk A, Lepczynski A, Dratwa-Chalupnik A, Kurpinska A, Klonowska A, Skrzypczak WF. Age-related changes of selected blood biochemical indicators in dairy calves during their first week of life. Folia Biol (Krakow) 2011; 59(1-2):25-30.
45. http://dx.doi.org/10.3409/fb59_1-2.25-30
47. Lisboa JAN, Benesi FJ, Leal MLR, Teixeira CMC.Leal. Efeito da idade sobre o equilibrio ácido básico de bezerras sadias no primeiro mes de vida. Braz J Vet Res Anim Sci 2002; 39(3):136-142.
48. http://dx.doi.org/10.1590/S1413-95962002000300005
50. Kirchner D, Schwedhelm L, Coenen M, Bachmann L. Dietary influences on the hydration and acid–base status of experimentally dehydrated dairy calves. Vet J 2014; 199(2):251-257.
51. http://dx.doi.org/10.1016/j.tvjl.2013.11.007
53. Poot A, Gillissen F, Koelmans A. Effects of flow regime and flooding on heavy metal availability in sediment and soil of a dynamic river system. Environ Pollut 2007; 148(3):779-787. doi:10.1016/j.envpol.2007.01.045
54. http://dx.doi.org/10.1016/j.envpol.2007.01.045
56. Effros RM. Stewart approach is not always a practical clinical tool. Anaesth Analg 2004; 98(1):271-272.
57. http://dx.doi.org/10.1213/01.ANE.0000077694.55641.80
59. Nakagawa, M, Suzuki K, Taguchi K. Relationship between depression score and acid-base status in Japanese black calves with diarrhea. J Vet Med Sci 2007; 69(5):549-552.
60. http://dx.doi.org/10.1292/jvms.69.549
62. Sobiech P, Rękawek W, Ali M, et al. Changes in blood acid-base balance parameters and coagulation profile during diarrhea in calves. Pol J Vet Sci 2013; 16(3):543-549.
63. http://dx.doi.org/10.2478/pjvs-2013-0075
65. Gentile A, Lorenz I, Sconza S, Klee W. Experimentally induced systemic hyperchloremic acidosis in calves. J Vet Intern Med 2008; 22(1):190-195. http://dx.doi.org/10.1111/j.1939-1676.2008.0028.x
67. Gómez De, Lofstedt J, Stämpfli HR, Wichtel M, Muirhead T, McClure JT. Contribution of unmeasured anions to acid–base disorders and its association with altered demeanor in 264 calves with neonatal diarrea. J Vet Intern Med 2013; 27(6):1604-1612.
68. http://dx.doi.org/10.1111/jvim.12193
70. Temiz M, Altuğ N, Yüksek N. Relationship between degree of anemia and blood gases in cattle with theileriosis. Turk J Vet Anim Sci 2014; 38:82-87. http://dx.doi.org/10.3906/vet-1305-75