Molecular detection and antimicrobial resistance of Aeromonas from houseflies (Musca domestica) in Iran

Molecular detection and antimicrobial resistance of Aeromonas from houseflies (Musca domestica) in Iran

ABSTRACT

Objective. This study aimed to report the molecular detection and antimicrobial resistance of Aeromonas among houseflies (Musca domestica) in Shahrekord and Isfahan provinces of Iran. Materials and methods. Flies were caught from household kitchens, cattle farms, animal hospitals, human hospitals, slaughter house and poultry farms and put in collection separate sterile tubes. Isolation was accomplished by culture of flies in alkaline peptone water followed by identification with Aeromonas-specific Polymerase Chain Reaction (PCR). Results. Out of 600 houseflies 73 (12.2%) were infected with Aeromonas spp. Significantly higher frequencies of Aeromonas were isolated in Shahrekord province (13.0%; 39/300) than in Isfahan province (11.3%; 34/300). The recovery frequencies of the organisms were significantly lower in kitchens as compared to those in cattle farms and hospital wards which were similar. Higher proportions of infected flies were obtained during summer whereas low proportions were obtained during winter. Conclusions. It is concluded that houseflies do harbor diarrheagenic pathogens, including Aeromonas especially during summer. The carried organisms are resistant to a number of antimicrobials at different levels. Thus, future plans aimed at stemming infections caused by these organisms should take flies into account. Control efforts of infections caused by this particular bacterium should therefore take into account Musca domestica.

1. Parker JL, Shaw JG. Aeromonas spp. clinical microbiology and disease. J Infect 2011; 62(2):109–118. http://dx.doi.org/10.1016/j.jinf.2010.12.003
3. Khamesipour F, Moradi M, Noshadi E, Momeni Shahraki M. Detection of the prevalence of Aeromonas hyrophila in shrimp samples by Polymerase Chain Reaction (PCR) and cultural method in the Iran. J Biodivers Environ Sci 2014; 4(2):47-52.
5. Chao CM, Gau SJ, Lai CC. Aeromonas genitourinary tract infection. J Infect 2012; 65(6):573–575. http://dx.doi.org/10.1016/j.jinf.2012.06.012
7. Chao CM, Lai CC, Tsai HY, Wu CJ, Tang HJ, Ko WC, Hsueh PR. Pneumonia caused by Aeromonas species in Taiwan. Eur J Clin Microbiol Infect 2013; 32(8):1069–1075. http://dx.doi.org/10.1007/s10096-013-1852-6
9. Lin WT, Su SY, Lai CC, Tsai TC, Gau SJ, Chao CM. Peritonitis caused by Aeromonas species at a hospital in southern Taiwan. Int Med 2013; 52(22):2517–21. http://dx.doi.org/10.2169/internalmedicine.52.0180
11. Nazni WA, Seleena B, Lee HL, Jeffery J, Rogayah TAR, Sofian MA. Bacteria fauna from the house fly, Musca domestica (L.). Trop Biomed 2005; 22(2):225-231.
13. Hemmatinezhad B, Ommi D, Hafshejani TT, Khamesipour F. Molecular detection and antimicrobial resistance of Pseudomonas aeruginosa from houseflies (Musca domestica) in Iran. J Venom Anim Toxins Incl Trop Dis 2015; 30(21):18. http://dx.doi.org/10.1186/s40409-015-0021-z
15. Nichlols GL. Fly transmission of Campylobacter. Emerg Infect Dis 2005; 11(3):361-364. http://dx.doi.org/10.3201/eid1103.040460
17. Wales AD, Carrique-Mas JJ, Rankin M, Bell B, Thind BB, Davies RH. Review of the carriage of zoonotic bacteria by arthropods, with special reference to Salmonella in mites, flies and litter beetles. Zoonoses and Public Health 2010; 57(5):299-314.
19. Nayduch D, Honko A, Noblet GP, Stutzenberger F. Detection of Aeromonas caviae in the common housefly Musca domestica by culture and polymerase chain reaction. Epidemiol Infect 2001; 127(3):561–566. http://dx.doi.org/10.1017/S0950268801006240
21. Sambrook J, D Russell: Molecular Cloning. a Laboratory Manual, 3rd edn. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory; 2001.
23. Standards NCFCL. Performance standards for antimicrobial disk and dilution susceptibility tests for bacteria isolated from animals; approved standard. A3 NdM- ed., 2 edition. Wayne, PA: National Committee for Clinical Laboratory Standards; 2008.
25. Humphrey T, O'Brien S, Madsen M. Campylobacters as zoonotic pathogens: A food production perspective. Int J Food Microbiol 2007; 117(3):237-257. http://dx.doi.org/10.1016/j.ijfoodmicro.2007.01.006
27. Kok J, O'Sullivan MV, Gilbert GL. Feedback to clinicians on preventable factors can reduce hospital onset Staphylococcus aureus bacteraemia rates. J Hosp Infect 2011; 79(2):108–114. http://dx.doi.org/10.1016/j.jhin.2011.04.023
29. Tang HJ, Lai CC, Lin HL, Chao CM. Clinical Manifestations of Bacteremia Caused by Aeromonas Species in Southern Taiwan. PLoS One 2014; 9(3):e91642. http://dx.doi.org/10.1371/journal.pone.0091642
31. Green C, Krause D, Wylie J. Spatial analysis of Campylobacter infection in the Canadian province of Manitoba. Int J Health Geogr 2006; 5: 2. doi: 10.1186/1476-072X-5-2. http://dx.doi.org/10.1186/1476-072X-5-2
33. Naumova EN: Mystery of seasonality. Getting the rhythm of nature. J Public Health Policy 2006; 27(1):2–12. http://dx.doi.org/10.1057/palgrave.jphp.3200061
35. Fisman DN. Seasonality of Infectious Diseases. Annu Rev Public Health 2007; 28:127–143. http://dx.doi.org/10.1146/annurev.publhealth.28.021406.144128
37. Naumova EN, Jagai JS, Matyas B, DeMaria A, MacNeill IB, Griffiths JK. Seasonality in six enterically transmitted diseases and ambient temperature. Epidemiol Infect 2007; 135(2):281–292. http://dx.doi.org/10.1017/S0950268806006698
39. Kovats RS, Edwards SJ, Charron D, Cowden J, D'Souza RM, Ebi KL, et al. Climate variability and Campylobacter infection: an international study. Int J Biometeorol 2005; 49(4):207–214. http://dx.doi.org/10.1007/s00484-004-0241-3
41. Meldrum RJ, Griffiths JK, Smith RM, Esnas MR. The seasonality of human Campylobacter infection and Campylobacter isolates from fresh, retail chicken in Wales. Epidemiol Infect 2005; 133(1):49-52. http://dx.doi.org/10.1017/S0950268804003188
43. Keegan VA, Majowicz SE, Pearl DL, Marshall BJ, Sittler N, Knowles L, Wilson JB. Epidemiology of enteric disease in C-EnterNet's pilot site - Waterloo region, Ontario, 1990 to 2004. CAN J Infect Dis Med 2009; 20(3):79–87. http://dx.doi.org/10.1155/2009/978784