Pythiosis cutaneous in horses treated with triamcinolone acetonide. Part 2. Histological and histochemical description

Pythiosis cutánea en equinos tratados con acetonida de triamcinolona. Parte 2. Descripción histológica e histoquímica

Objective. The study aimed to evaluate the histomorphometry tissue recovery process of the skin granuloma of skin pythiosis in horses treated with triamcinolone acetonide. Materials and methods. We conducted a descriptive study, not probabilistic in convenience animals with cutaneous pythiosis. 24 horses were used with cutaneous pythiosis, a group of 12 animals was administered 50 mg of intramuscular injection of triamcinolone acetonide (TG) and the other group was not applied any treatment (CG). Are tissue biopsies performed for histological and histochemical evaluation and stained with hematoxylin eosin (HE), Gomori trichrome (GT), picrosirius red polarization (PR/P), Grocott methenamine silver (GMS) and periodic acid-Schiff (PAS). Results. It is noted that in TG inflammation was gradually decreasing, as evidenced in decreased fibrin layer leukocyte, PMN and phenomena Splendore Hoepli, also in increased angiogenesis, epiteliogénesis, and increasing the overall amount of fibroblasts and collagen fibers, anyway in the progressive replacement of collagen type III to type I collagen at the end of the process, and that the presence of intralesional pseudo-hyphae of Pythium insidiosum reduces it to the second week. Neither of the animals in the CG showed improvement in histological and histochemical characteristics of pythiosis and maintained equal to the first day throughout the study. Conclusions. The use of triamcinolone acetonide is a good therapeutic alternative for the treatment of granulomatous pythiosis wounds in horses with 100% clinical recovery and demonstrated with histological and histochemical findings.

1. Cardona J, Vargas M, Perdomo S. Frecuencia de Pythiosis cutánea en caballos de producción en explotaciones ganaderas de Córdoba, Colombia. Rev Med Vet Zoot 2014; 61(I):31-43.
2. Santos C, Santurio J, Marques C. Pitiose em animais de produção no Pantanal Matogrossense. Pesq Vet Bras 2011; 31(12):1083-89.
3. White S. Equine Bacterial and Fungal Diseases: A Diagnostic and Therapeutic Update. Clin Tech Equine Pract 2005; 4:302-310.
4. Fonseca A, Botton S, Nogueira C, Correa B, Silveira J, Azevedo M, Maroneze B, Santurio J, Pereira D. In Vitro Reproduction of the Life Cycle of Pythium insidiosum from Kunkers’ Equine and Their Role in the Epidemiology of Pythiosis. Mycopathologia 2014; 177:123–127.
5. Cardona J, Vargas M, González M. Evaluación clínica e histopatológica de la pythiosis cutánea en terneros del departamento de Córdoba, Colombia. Rev MVZ Córdoba 2013a; 18(2):3551-3558.
6. Cardona J, Vargas M, Perdomo S. Pythiosis cutánea equina: una revisión. Rev Ces Med Vet Zootec 2013b; 8(1):58-67.
7. Márquez A, Salas Y, Canelón J, Perazzo Y, Colmenárez V. Descripción anatomopatológica de pitiosis cutánea en equinos. Rev Fac Cs Vets UCV 2010; 51(1):37-42.
8. Luis-León J, Pérez R. Pythiosis: Una patología emergente en Venezuela. Salus online 2011; 15(1):79-94.
9. Mrad A. Ética en la investigación con modelos animales experimentales. Alternativas y las 3 RS de Russel. Una responsabilidad y un compromiso ético que nos compete a todos. Rev Col Bioética 2006; 1(1):163-184.
10. Pabón J, Eslava J, Gómez R. Generalidades de la distribución espacial y temporal de la temperatura del aire y de la precipitación en Colombia. Meteorol. Colomb 2001; 4:47-59.
11. Dória R, Freitas S, Mendonça F, Arruda L, Boabaid F, Filho A, Colodel E, Valadão E. Utilização da técnica de imuno-histoquímica para confirmar casos de pitiose cutânea equina diagnosticados por meio de caracterização clínica e avaliação histopatológica. Arq Bras Med Vet Zootec 2014; 66(1):27-33.
12. Galiza G, da Silva T, Caprioli R, Barros C, Irigoyen L, Fighera R, Lovato M, Kommers G. Ocorrência de micoses e pitiose em animais domésticos: 230 casos. Pesq Vet Bras 2014; 34(3):224-232.
13. Frey F, Velho J, Lins L, Nogueira C, Santurio J. Pitiose equina na região sul do Brasil. Rev Port Cienc Vet 2007; 102:107–111.
14. Pereira D, Santurio J, Alves S, Argenta J, Potter L, Spanamberg A, Ferreiro L. Caspofungin in vitro and in vivo activity against Brazilian Pythium insidiosum strains isolated from animals. J Antimicrob Chemother 2007; 60:1168–1171.
15. Argenta J. Atividade in vitro, individual ou em combinação, de voriconazol, itraconazol e terbinafina contra isolados brasileiros de Pythium insidiosum. Acta Scientiae Veterinariae 2008; 36(3):327-328.
16. Cavalheiro A, Maboni G, Azevedo M, Argenta J, Pereira D, Spader T, Alves S, Santurio J. In Vitro Activity of Terbinafine Combined with Caspofungin and Azoles against Pythium insidiosum. Antimicrob. Agents Chemother 2009; 53(5):2136–2138.
17. Biava J, Ollhoff D, Gonçalves R, Biondo A. Zigomicose em equinos-revisão. Rev Acad Curitiba 2007; 5:225-230.
18. Bandeira A, Santos J, Melo M, Andrade V, Dantas A, Araujo J. Pitiose equina no estado de sergipe, Brasil. Ciênc Vet Tróp Recife-PE 2009; 12(1):46-54.
19. Loreto E, Nunes-Mario D, Denardi L, Alves S, Santurio J. In Vitro Susceptibility of Pythium insidiosum to Macrolides and Tetracycline Antibiotics. Antimicrob. Agents Chemother 2011; 55(7):3588–3590.
20. Santurio J, Alves S, Pereira D, Argenta J. Pitiose: uma micose emergente. Act Sci Vet 2006; 34(1):1-14.
21. Mendoz L, Newton J. Immonology and immunotherapy of the infections caused by Pythium insidiosum. Medical Mycology 2005; 43:477-486.
22. Gaastra W, Lipman L, De Cock A, Exel T, Pegge R, Scheurwater J, Vilela R, Mendoza L. Pythium insidiosum: An Overview. Vet Microbiol 2010; 146:1-16.
23. Liberman A, Druker J, Perone M, Arzt E. Glucocorticoids in the regulation of transcription factors that control cytokine synthesis. Cytokine Growth Factor Rev 2007; 18:45-56.
24. Liberman A, Druker J, Refojo D, Arzt E. Mecanismos moleculares de accion de algunas drogas inmunosupresoras. Medicina 2008; 68(6):455-464.
25. Meagher L, Cousin J, Seckl J, Haslett C. Opposing effects of glucocorticoids on the rate of apoptosis in neutrophilic and eosinophilic Granulocytes. J immunol 1996; 156: 4422-4428.
26. Ramírez G. Fisiología de la cicatrización: Art de Revisión. Rev Fac Salud 2010; 2(2):69-78.
27. Mahdavian B, van der Veer W, van Egmonda M, Niessenb F, Beelena R. Macrophages in skin injury and repair. Immunobiology 2011; 216:753–762.
28. Niessen F, Andriessen M, Schalkwijk J, Visser L, Timens W. Keratinocyte-derived growth factors play a role in the formation of hypertrophic scars. J Pathol 2001; 194:207–216.
29. Coleman R. Picrosirius red staining revisited. Acta histochemical 2011; 113:231–233.
30. Ruiz J. 2001. Factores fisiológicos que modifican la acción de los fármacos en medicina veterinaria. Rev Col Cienc Pec 14(1):36–48.