EspañolDetection of antibodies against Neospora caninum in canines in the urban and rural area of Cumaral, Meta, Colombia

Detección de anticuerpos contra Neospora caninum en caninos del área urbana y rural de Cumaral, Meta-Colombia

Objective. To estimate the seroprevalence to Neospora caninum in canines of the urban and rural area of Cumaral, Meta and determine some risk factors associated with seropositivity. Materials and methods. A cross-sectional study was carried out in 222 dogs (112 dogs from the urban area and 110 dogs from the rural area), the sample size was calculated by using Epidat v program. 3.1. The sera were analyzed using the Indirect Immunofluorescence technique for IgG with a commercial kit. Frequency analyzes by chi-square of independence were performed in SPSS v. 25.0. Results. The general seroprevalence was 36.9% (95% CI: 30.9-43.5%). The seropositivity between the groups was: urban (38.4%) and rural (35.5%) (p>0.05), males (36.9%) and females (36.9%) (p>0.05); in puppies (32.7%), youth (40.0%) and adults (37.4%) (p>0.05), in contact with livestock farms (40.7%) and without contact (35.2 %) (p>0.05). Conclusions. The seroprevalence observed was high in the two populations analyzed and suggests that the canines have been in contact with the parasite, possibly due to different sources of infection that need to be studied later.

1. Dubey JP, Schares G, Ortega-Mora LM. Epidemiology and control of neosporosis and Neospora caninum. Clinic Microbiol Rev. 2007; 20(2):323-367. https://doi.org/10.1128/CMR.00031-06
2. Regidor-Cerrillo J, Díez-Fuertes F, García-Culebras A, Moore DP, González-Warleta M, Cuevas C, et al. Genetic diversity and geographic population structure of bovine Neospora caninum by microsatellite genotyping analysis. PLoS ONE. 2013; 8(8):e72678. https://doi.org/10.1371/journal.pone.0072678
3. Prandini da Costa Reis R, Crisman R, Roser M, Malik R, Slapeta J. Neonatal neosporosis in a 2-week-old Bernese mountain dog infected with multiple Neospora caninum strains based on MS10 microsatellite analysis. Vet Parasitol. 2016; 15(221):134–138. https://doi.org/10.1016/j.vetpar.2016.03.023
4. Nazir MM, Maqbool A, Akhtar M, Ayaz M, Ahmad AN, Ashraf K, et al. Neospora caninum prevalence in dogs raised under different living conditions. Vet Parasitol. 2014; 204(3–4):364–368. https://doi.org/10.1016/j.vetpar.2014.05.041
5. Arunvipas P, Inpankaew T, Jittapalapong S. Risk factors of Neospora caninum infection in dogs and cats in dairy farms in Western Thailand. Trop Anim Health Prod. 2012; 44(5): 1117–1121. https://doi.org/10.1007/s11250-011-0048-2
6. Dubey JP, Schares G, Ortega-Mora LM. Epidemiology and Control of Neosporosis and Neospora caninum. Clinic Microbiol Rev. 2007; 20(2):323–367. https://doi.org/10.1128/CMR.00031-06
7. Langoni H, Matteucci G, Medici B, Camossi LG, Richini- Pereira VB, Silva RC. Detection and molecular analysis of Toxoplasma gondii and Neospora caninum from dogs with neurological disorders. Rev Soc Bras Med Trop. 2012; 45(3):365–368. http://dx.doi.org/10.1590/S0037-86822012000300016
8. Ribeiro CM, Soares IR, Mendes RG, de Santis Bastos PA, Katagiri S, Renato Zavilenski RB. Meta-analysis of the prevalence and risk factors associated with bovine neosporosis. Tropical Animal Health and Production. 2019; 51(7):1783-1800 https://doi.org/10.1007/s11250-019-01929-8
9. Cardona JA, Martínez Y, Betancur CA. Seroepidemiología de hembras bovinas naturalmente infectadas por Neospora caninum en Córdoba, Colombia. Rev UDCA Act & Div Cient. 2015; 18(2):401-408 https://revistas.udca.edu.co/index.php/ruadc/article/view/166/1281
11. Vargas-Niño A, Vargas J, Parra J, Vásquez M, Góngora A, Mogollón-Waltero EM. Estado serológico a IBR, DVB, Leucosis, Leptospira y Neospora caninum en hembras bovinas del Departamento de Santander, Colombia. Rev MVZ Córdoba. 2018; 23(2):6671-6680. https://doi.org/10.21897/rmvz.1341
12. Silva R, Machado G. Canine neosporosis: perspectives on pathogenesis and management. Vet Med. 2016; 26(7):59–70. https://doi.org/10.2147/VMRR.S76969
13. Ministerio de Salud. Reporte de vacunación antirrábica de perros y gatos, Colombia 2017. En http://www.minsalud.gov.co/sites/rid/Lists/BibliotecaDigital/RIDE/VS/PP/SA/nacional-municipio-2017.pdf. Revisado 5/02/2020
14. IDEAM, 2014. Atlas climatológico de Colombia. Instituto de Hidrología, Meteorología y Estudios Ambientales (IDEAM), Bogotá, Colombia. http://documentacion.ideam.gov.co/cgi-bin/koha/opac-detail.pl?biblionumber=1381&shelfbrowse_itemnumber=1465
15. EPIDAT 3.1. 2006. Programa para análisis de datos epidemiológicos tabulados. Versión 3.1. Xunta de Galicia, Conselleria de sanidade-Organización Panamericana de la salud. OPS. https://www.sergas.es/Saude-publica/Epidat-3-1-descargar-Epidat-3-1-(espanol)
16. Newcombe RG, Merino C. Intervalos de confianza para las estimaciones de proporciones y las diferencias entre ellas. Interdisciplinaria. 2006; 23 (2):141-154.
17. Haddadzadeh HR, Sadrebazzaz A. Malmasi A, Ardakani HT, Nia PK, Sadreshirazi N. Seroprevalence of Neospora caninum infection in dogs from rural and urban environments in Tehran, Iran. Parasitol Res. 2007; 101 (6):1563–1565. https://doi.org/10.1007/s00436-007-0678-5
18. Ferroglio E, Pasino M, Ronco F, Bena A, Trisciuoglio. A Seroprevalence of Antibodies to Neospora caninum in Urban and Rural Dogs in north-west Italy. Zoonoses Public Health. 2007; 54:135–139. https://doi.org/10.1111/j.1863-2378.2007.01033.x
19. Robbe D, Passarelli A, Gloria A, Di Cesare A, Capelli G, Iorio R, Traversa D. Neospora caninum seropositivity and reproductive risk factors in dogs. Exp Parasitol. 2016; 164:31-35. https://doi.org/10.1016/j.exppara.2016.02.003
20. Nogueira CI, Mesquita LP, Abreu CC, Nakagaki KY, Seixas JN, Bezerra PS, et al. Risk factors associated with seroprevalence of Neospora caninum in dogs from urban and rural areas of milk and coffee production in Minas Gerais State, Brazil. Epidemiol Infect. 2013; 141(11):2286–2293. https://doi.org/10.1017/S0950268813000162
21. Basso W, Venturini L, Venturini M, Moore P, Rambeau M, Unzaga J, et al. Prevalence of Neospora caninum infection in dogs from beef cattle farms, dairy farms, and from urban areas of Argentina. J Parasitol. 2001; 87(4):906-907. https://doi.org/10.1645/0022-3395(2001)087[0906:PONCII]2.0.CO;2
22. Sawada M, Park CH, Kondo H, Morita T, Shimada A, Yamane I, et al. Serological survey of antibody to Neospora caninum in Japanese dogs. J Vet Med Sci. 1998; 60(7):853-854. https://doi.org/10.1292/jvms.60.853
23. Wouda W, Dijkstra T, Kramer AM, van Maanen C, Brinkhof JM. Seroepidemiological evidence for a relationship between Neospora caninum infections in dogs and cattle. Int J Parasitol. 1999; 29(10):1677-1682. https://doi.org/10.1016/s0020-7519(99)00105-8
24. Wang S, Yao Z, Zhang N, Wang D, Ma J, Liu S, Zheng B, Zhang B, Liu K, Zhang H. Serological study of Neospora caninum infection in dogs in central China. Parasite. 2016, 23(25):1-5. https://doi.org/10.1051/parasite/2016025
25. Patitucci AN, Phil M, Pérez MJ, Rozas MA, Israel KF. Neosporosis canine: detection of sera antibodies in rural and urban canine population of Chile. Arch Med Vet. 2001; 33 (2):227-232. http://dx.doi.org/10.4067/S0301-732X2001000200011
26. Escalona JJ, Corro AC, Suárez CE, Castillo TA, Pineda YA. Seropositividad a Neospora caninum en perros de áreas rurales y urbanas del estado Yaracuy, Venezuela. Rev Fac Cs Vets. UCV. 2013; 54(1):29-34. http://saber.ucv.ve/ojs/index.php/revisfcv/article/view/5146/4953
27. Gozdzik K, Wrzesien R, Wielgosz-Ostolska A, Bien J, Kozak- Ljunggren M, Cabaj W. Prevalence of antibodies against Neospora caninum in dogs from urban areas in Central Poland. Parasitology Research. 2011; 108(4):991–996. https://doi.org/10.1007/s00436-010-2143-0
28. Villagra-Blanco R, Angelova L, Conze T, Schares G, Bärwald A, Taubert A, Hermosilla C, Wehrend A. Seroprevalence of Neospora caninum-specific antibodies in German breeding bitches. Parasites & Vectors. 2018; 11(96):2-6. https://doi.org/10.1186/s13071-018-2683-1
29. Sloan S, Šlapeta J, Jabbar A, Hunnam J, De Groef B, Rawlin G, McCowan C. High seroprevalance of Neospora caninum in dogs in Victoria, Australia, compared to 20 years ago. Parasites & Vectors. 2017; 10(1):503. https://doi.org/10.1186/s13071-017-2464-2
30. Bulla-Blanco KD, Vanegas-Rojas PX. (2015). Identificación de neospora caninum en materia fecal de 60 caninos en hatos lecheros en el municipio el Rosal, Cundinamarca. Retrieved from https://ciencia.lasalle.edu.co/medicina_veterinaria/104
31. Cedeño D, Benavides B. Seroprevalencia y factores de riesgo asociados a Neospora caninum en ganado lechero en el municipio de Pasto, Colombia. Rev MVZ Córboba. 2013; 18(1):3311-3316. https://doi.org/10.21897/rmvz.193
32. Ravelo-Salcedo L. (2009). Estudio clínico, serológico y coprológico preliminar de Neospora Caninum en caninos de la clínica veterinaria Dover Bogotá - Colombia. Retrieved from https://ciencia.lasalle.edu.co/medicina_veterinaria/296
33. Barber JS, Trees AJ. Naturally ocurring vertical transmission of Neospora caninum in dogs. Int J Parasitol. 1998; 28:57-64. https://doi.org/10.1016/s0020-7519(97)00171-9
34. McInnes LM, Irwin P, Palmer DG, Ryan UM. In vitro isolation and characterization of the first canine Neospora caninum isolate in Australia. Vet Parasitol. 2006; 137:355–363. https://doi.org/10.1016/j.vetpar.2006.01.018
35. Rangel O. La biodiversidad de Colombia: significado y distribución regional. Rev Acad Colomb Cienc Ex Fis Nat. 2015; 39(151):176-200. http://dx.doi.org/10.18257/raccefyn.136
36. Donahoe SL, Lindsay SA, Krockenberger M, Phalen D, Šlapeta J. A review of neosporosis and pathologic findings of Neospora caninum infection in wildlife. Int J Parasitol Parasites Wildl. 2015; 4:216–38. http://doi.org/10.1016/j.ijppaw.2015.04.002
37. Atkinson R, Harper PA, Reichel MP, Ellis JT. Progress in the serodiagnosis of Neospora caninum infections of cattle. Parasitol Today. 2000; 16(3):110–114. https://doi.org/10.1016/s0169-4758(99)01604-x
38. Campero LM, Minke L, Moré G, Rambeaud M, Bacigalupe D, Moore DP, et al. Evaluation and comparison of serological methods for the detection of bovine neosporosis in Argentina. Rev Argent Microbiol. 2015; 47(4):295-301. https://doi.org/10.1016/j.ram.2015.07.002
39. Hemphill A, Gottstein B. A European perspective on Neospora caninum. Int J Parasitol. 2000; 30:877–924. https://doi.org/10.1016/S0020-7519(00)000722