Comparación de dos protocolos de inseminación artificial a tiempo fijo en vacas Brahman

Comparison between two fixed-time artificial insemination protocols in Brahman cows

Objetivo. Comparar dos protocolos de inseminación artificial a tiempo fijo (IATF) sobre el porcentaje de preñez en vacas Brahman. Materiales y métodos. Se seleccionaron 60 vacas entre 90 y 360 días posparto y asignadas aleatoriamente en dos grupos. El tratamiento 1 (T1; n=30) consistió en la aplicación el día 0 de un dispositivo intravaginal (DIV) que contenía 0.5 g de progesterona y 2.0 mg de benzoato de estradiol im. El día 8 se retiró el DIV y se aplicaron 0.150 mg de PgF2α, im y 300 UI de eCG, im y 0.5 mg de cipionato de estradiol, im. El día 10 se realizó la IATF a las 48 – 50 h posterior al retiro del DIV. El tratamiento 2 (T2; n= 30) consistió en la inserción del DIV el día 0 y 0.01 mg GnRH, im. El día 5 se retiró el DIV y se aplicaron dos dosis de 0.150 mg de PgF2α, a las 0 y 12 horas. El día 8 se realizó la IATF a las 72 h posterior al retiro del DIV y 0.01 mg de GnRH, im. La IATF se realizó con un semen de fertilidad comprobada. Resultados. El diagnóstico de preñez se realizó a los 45 días luego de la IATF por palpación rectal. El T2 y T1 presentaron porcentajes de preñez de (46.7%; 14/30) y (33.3%; 10%30), respectivamente. Conclusiones. Se justifica la aplicación del tratamiento 2 en función de los resultados y la salud pública.

1. Sartori R, Barros CM. Reproductive cycles in Bos indicus cattle. Anim Reprod Sci 2011; 124(3):244–250. https://doi.org/10.1016/j.anireprosci.2011.02.006
3. Gimenes LU, Sá Filho MF, Carvalho NA, Torres-Júnior JR, Souza AH, Madureira EH, et al. Follicle deviation and ovulatory capacity in Bos indicus heifers. Theriogenology 2008; 69(7):852–858. https://doi.org/10.1016/j.theriogenology.2008.01.001
5. Uribe F, Zuluaga AF, Valencia L, Murgueitio E, Ochoa L. Buenas Prácticas Ganaderas. Proyecto Ganadería Colombiana Sostenible GEF, BANCO MUNDIAL, FEDEGAN, CIPAV, FONDO ACCION, TNC. Bogotá, Colombia 2011; Man (3):82.
7. Garcia-Ispierto I, Lopez-Gatius F. A three-day PGF2α plus eCG based fixed-time AI protocol improves fertility compared with spontaneous estrus in dairy cows with silent ovulation. J Reprod Dev 2013; 59(4):393-397. https://doi.org/10.1262/jrd.2013-017
9. Odde K G. A review of syncronization of estrus in pospartum cattle. J Anim Sci 1990; 68(3):817-830. https://doi.org/10.2527/1990.683817x
11. Bicalho RC, Galvao KN, Guard CL, Santos JEP. Optimizing theaccuracy of detecting a functional corpus luteum in dairy cows. Theriogenology 2008; 70(2):199–207. https://doi.org/10.1016/j.theriogenology.2008.03.015
13. Bó GA, Cutaia L, Peres LC, Pincinato D, Mara-a D, Baruselli PS. Technologies for fixed-time artificial insemination and their influenceon reproductive performance of Bos indicus cattle. Soc Reprod Fertil 2007; 64(Supl):223–236.
15. Figueira PM, Ereno RL, Satrapa RA, Pinheiro PG, Trinca LA, Barros CM. Neither plasma progesterone concentrations nor exogenous eCG affects rates of ovulation or pregnancy in fixed-time artificial insemination (FTAI) protocols for puberal Nellore heifers. Theriogenology 2011; 75(1):17-23. https://doi.org/10.1016/j.theriogenology.2010.07.005
17. Carvalho JBP, Carvalho NAT, Reis EL, Nichi M, Souza AH, Baruselli PS. Effect of early luteolisis in progesterone-based timed AI protocols in Bos indicus, Bos indicus x Bos taurus, and Bos taurus heifers. Theriogenology 2008; 69(2):167-175. https://doi.org/10.1016/j.theriogenology.2007.08.035
19. Sá-Filho MF, Crespilho AM, Santos JEP, Perry GA, Baruselli PS. Ovarian follicle diameter at timed insemination and estrous response influence likelihood of ovulation and pregnancy after estrous synchronization with progesterone or progestin-based protocols in suckled Bos indicus cows. Anim Reprod Scien 2010; 120(1):23-30. https://doi.org/10.1016/j.anireprosci.2010.03.007
21. Meneghetti M, Sá-Filho OG, Peres RFG, Lamb GC, Vasconcelos JLM. Fixed-time artificial insemination with estradiol and progesterone for Bos indicus cows I: Basis for development of protocols. Theriogenology 2009; 72(2):179-189. https://doi.org/10.1016/j.theriogenology.2009.02.010
23. Uribe-Velásquez L, Correa-Orozco A, Cuartas-Betancurth L, Villamizar-Ramirez D, Ángel-Botero S. Evaluation of reused norgestomet implants in estrus synchronization protocols in Brahman cows. Rev MVZ Córdoba 2013; 18(1):3336-3345. https://doi.org/10.21897/rmvz.196
25. Bridges GA, Helser LA, Grum DE, Mussard ML, Gasser CL, Day ML. Decreasing the interval between GnRH and PGF2α from 7 to 5 days and lengthening proestrus increases timed-AI pregnancy rates beef cows. Theriogenology 2008; 69(7):843-851. https://doi.org/10.1016/j.theriogenology.2007.12.011
27. Prada JA, Castro JA, Ardila A, Chacón J. Evaluación de un protocolo de inseminación artificial a tiempo fijo con variaciones en los días de aplicada la dosis de prostaglandina en novillas Brahman puras y cruzadas. Rev Cien Anim 2013; 6:161-175.
29. Dorneles TR, Ferreira R, Tonellotto DSJ, Silveira O, Barreta MH, Oliveira JF. The effect of equine chorionic gonadotropin on follicular size, luteal volume, circulating progesterone concentrations, and pregnancy rates in anestrous beef cows treated with a novel fixed-time artificial insemination protocol. Theriogenology 2013; 79(8):1204–1209. https://doi.org/10.1016/j.theriogenology.2013.02.019
31. Meneghetti M, Vasconcelos JML. Calving date, body condition score, and response to a timed artificial insemination protocol in first calving beef cows. Arq Bras Met Vet Zootec 2008; 60(4):786-793. https://doi.org/10.1590/S0102-09352008000400002
33. Ribeiro ES, Bisinotto RS, Lima FS, Greco LF, Morrison A, Kumar A, et al. Plasma anti-Müllerian hormone in adult dairy cows and associations with fertility. J dairy scien 2014; 97(11): 6888-6900. https://doi.org/10.3168/jds.2014-7908
35. Newcombe R, Merino S. Intervalos de confianza para las estimaciones de proporciones y las diferencias entre ellas. Interdisciplinaria 2006; 23(2):141-154.
37. Villa N, Morales C, Granada J, Mesa H, Gomez G, Molina J. Evaluación de cuatro protocolos de sincronización para inseminación a tiempo fijo en vacas Bos taurus indicus lactantes. Rev Cien 2007; 17(5): 501-507.
39. Pugliesi G, Santos FB, Lopes E, Nogueira É, Maio JRG, Binelli M. Improved fertility in suckled beef cows ovulating large follicles or supplemented with long-acting progesterone after timed-AI. Theriogenology 2016; 85(7):1239–1248. https://doi.org/10.1016/j.theriogenology.2015.12.006
41. Peralta-Torres J, Aké-López J, Centurión-Castro F, Magana-Monforte J. Comparison of estradiol cypionate and estradiol benzoateeffects on ovaric activity, estrus and ovulation on anestrus Bos indicuscows. J Anim Vet Adv 2010; 9(3):466–470. https://doi.org/10.3923/javaa.2010.466.470
43. Salgado R, Gonzalez M, Simanca J. Inseminación artificial a tiempo fijo en vacas brahman lactantes. Rev MVZ Córdoba 2007, 12(2):1050-1053.
45. Rabaglino MB, Risco CA, Thatcher MJ, Lima F, Santos JEP, Thatcher WW. Use of a five-day progesterone-based timed AI protocol to determine if flunixinmeglumine improves pregnancy per timed AI in dairy heifers. Theriogenology 2010; 73(9):1311-1318. https://doi.org/10.1016/j.theriogenology.2009.12.012
47. Modric D, Momcilovic WE, Gwin AT, Peter. Hormonal and antimicrobial therapy in theriogenology practice: Currently approved drugs in the USA and possible future directions. Theriogenology 2011; 76(3):393-408. https://doi.org/10.1016/j.theriogenology.2011.02.005
49. Díaz GS, Galina CS, Basurto CH. Efecto de la progesterona natural con o sin la adición de benzoato de estradiol sobre la presentación de celo, ovulación y gestación en animales tipo Bos indicus en el trópico mexicano. Arch Med Vet 2002; 34(2):283-286. https://doi.org/10.4067/S0301-732X2002000200009
51. Uribe-Velásquez L, Correa-Orozco A, Cuartas-Betancurth L, Villamizar-Ramirez D, Ángel-Botero S. Evaluation of reused norgestomet implants in estrus synchronization protocols in Brahman cows. Rev MVZ Córdoba 2013; 18(1):3336-3345. https://doi.org/10.21897/rmvz.196
53. Pinheiro V, Souza A, Pegorer M, Ereno R, Barros C. Do temporary calf removal (TCR) and/or eCG administration increase pregnancy rates in lactating Nelore cows treated with progesterone release intravaginal device. Acta Scien Vet 2005; 33(Supl):280.
55. Fanning M, Lunt D, Sprott L, Forrest D. Reproductive performance of synchronized beef cows as affected by inhibition of suckling with nose tags or temporary calf removal. Theriogenology 1995; 44(5):715-723. https://doi.org/10.1016/0093-691X(95)00251-3
57. Dadarwal D, Mapletoft RJ, Adams GP, Pfeifer LF, Creelman C, Singh J. Effect of progesterone concentration and duration of proestrus on fertility in beef cattle after fixed-time artificial insemination. Theriogenology 2013; 79(5):859-866. https://doi.org/10.1016/j.theriogenology.2013.01.003
59. Sales JN, Carvalho JB, Crepaldi GA, Cipriano RS, Jacomini JO, Maio JR, et al. Effects of two estradiol esters (benzoate and cypionate) on the induction of synchronized ovulations in Bos indicus cows submitted to a timed artificial insemination protocol. Theriogenology 2012; 78(3):510–516. https://doi.org/10.1016/j.theriogenology.2012.02.031