Efecto de diferentes concentraciones de Albúmina Sérica Bovina en la congelabilidad del semen caprino

Effect of different concentrations of bovine serum albumin on freezing of goat semen

Objetivo. Evaluar en un diluyo-conservador liofilizado a base de Tris, el efecto de la concentración de Sero Albúmina Bovina (BSA) en la congelabilidad del semen caprino, sin realizar el lavado seminal, comparado con un diluyo-conservador control de lactosa-leche descremada (DC), eliminando el plasma seminal por centrifugación. Materiales y métodos. Se determinó en 90 eyaculados, volumen, motilidad, concentración, viabilidad y morfología espermática. Los eyaculados aptos, fueron mezclados y el pool dividido en cinco porciones, cada una recibió una de 4 combinaciones liofilizados a base de Tris-Glucosa-Ac. Cítrico y Glicerol, con diferentes concentraciones de BSA (0.1, 0.5%, 1 y 2%) o DC. Se congeló en pastillas de 0.1 ml en vapores de nitrógeno, y transcurridos 2 min., fueron almacenadas en nitrógeno líquido hasta su descongelación, 7 días después, se determinaron: porcentaje de motilidad (30, 120 y 240 minutos), viabilidad, acrosomas dañados y anomalías totales (30 y 120 minutos) y se compararon mediante un modelo de Regresión Logística Binaria. Resultados. La mayor motilidad espermática y viabilidad (p<0.05) en los tres tiempos fue para 0.5%, 1% y 2 % de BSA, que fueron superiores al 0.1% de BSA y DC. Los acrosomas dañados y laanomalías totales a los 30 y 120 minutos fueron menores (p<0.05) para 0.5%, 1% y 2 % de BSA en comparación con el 0.1% y DC. Conclusiones. La crioconservación del semen caprino, no requiere lavado seminal por centrifugación si se utiliza como protector de membrana BSA al 0.5-2% en un diluyo-conservador liofilizado a base de Tris-Glucosa-Ácido Cítrico y Glicerol.

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