Efecto de diferentes concentraciones de Albúmina Sérica Bovina en la congelabilidad del semen caprino
Effect of different concentrations of bovine serum albumin on freezing of goat semen
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Objetivo. Evaluar en un diluyo-conservador liofilizado a base de Tris, el efecto de la concentración de Sero Albúmina Bovina (BSA) en la congelabilidad del semen caprino, sin realizar el lavado seminal, comparado con un diluyo-conservador control de lactosa-leche descremada (DC), eliminando el plasma seminal por centrifugación. Materiales y métodos. Se determinó en 90 eyaculados, volumen, motilidad, concentración, viabilidad y morfología espermática. Los eyaculados aptos, fueron mezclados y el pool dividido en cinco porciones, cada una recibió una de 4 combinaciones liofilizados a base de Tris-Glucosa-Ac. Cítrico y Glicerol, con diferentes concentraciones de BSA (0.1, 0.5%, 1 y 2%) o DC. Se congeló en pastillas de 0.1 ml en vapores de nitrógeno, y transcurridos 2 min., fueron almacenadas en nitrógeno líquido hasta su descongelación, 7 días después, se determinaron: porcentaje de motilidad (30, 120 y 240 minutos), viabilidad, acrosomas dañados y anomalías totales (30 y 120 minutos) y se compararon mediante un modelo de Regresión Logística Binaria. Resultados. La mayor motilidad espermática y viabilidad (p<0.05) en los tres tiempos fue para 0.5%, 1% y 2 % de BSA, que fueron superiores al 0.1% de BSA y DC. Los acrosomas dañados y laanomalías totales a los 30 y 120 minutos fueron menores (p<0.05) para 0.5%, 1% y 2 % de BSA en comparación con el 0.1% y DC. Conclusiones. La crioconservación del semen caprino, no requiere lavado seminal por centrifugación si se utiliza como protector de membrana BSA al 0.5-2% en un diluyo-conservador liofilizado a base de Tris-Glucosa-Ácido Cítrico y Glicerol.
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