Effect of different concentrations of bovine serum albumin on freezing of goat semen

Efecto de diferentes concentraciones de Albúmina Sérica Bovina en la congelabilidad del semen caprino

Objective. Evaluate the effect of Bovine Serum Albumin (BSA) concentration on the freezability of goat semen in a Tris-based lyophilized preservative-diluter, without performing seminal washing, compared with a control preservative-diluter of lactose-skimmed milk (DC), removing the seminal plasma by centrifugation. Materials and methods. It will cover 90 ejaculates, volume, motility, concentration, viability, and sperm morphology. The fit ejaculates were mixed and the pool divided into five portions, each receiving one of 4 lyophilized combinations based on Tris-Glucose-Ac. Citrus and Glycerol with different concentrations of BSA (0.1%, 0.5%, 1% and 2%) or DC. It was frozen in 0.1 ml pellets in nitrogen vapors, and after 2 min., They were stored in liquid nitrogen until thawing 7 days later, the following were determined: motility percentage (30, 120 and 240 minutes), viability, damaged acrosomes and total anomalies (30 and 120 minutes) and were compared using a Binary Logistic Regression model. Results. The highest sperm motility and viability (p <0.05) in the three times was for 0.5%, 1% and 2% of BSA, which were higher than 0.1% of BSA and DC. Damages acrosome and total abnormalities at 30 and 120 minutes were lower (p <0.05) for 0.5%, 1% and 2% of BSA compared to 0.1% and DC. Conclusion. The cryopreservation of goat semen does not require seminal washing by centrifugation if 0.5-2% BSA is used as a membrane protector in a lyophilized dilute-conservative based on Tris-Glucose-Citric Acid and Glycerol.

1. - Sharma A, Sood P. Caprine semen cryopreservation and the factors affecting it: An overview. Vet Sci Res Rev. 2020; 6(1):46-57. http://dx.doi.org/10.17582/journal.vsrr/2020/6.1.46.57
2. - Santiago-Moreno J, Galarza DA. Sperm cryopreservation in domestic and wild species: a review of recent advances. Rev Ec Ciencia An. 2019; 3(2):18-38. http://www.revistaecuatorianadecienciaanimal.com/index.php/RECA/article/view/116
3. - Gangwar C, Kharche SD, Kumar S, Jindal SK. Cryopreservation of goat semen: status and prospects. Indian J. Small Ruminants. 2016;22(1):1-10. http://www.indianjournals.com/ijor.aspx?target=ijor:ijsr&volume=22&issue=1&article=001
4. - Umar S, Ahmad M, Ahmad I, Zubair M, Umar Z, Qureshi AS, Manzoor A, Murtaza A, Shaukat A. Correlation of biochemical constituents of seminal plasma with semen quality in Teddy goat (Capra hircus) bucks. Andrología. 2018; 50(3):e12940. https://doi.org/10.1111/and.12940
5. - Ritar AJ, Salamon S. Effects of seminal plasma and its removal and of egg yolk in the diluent on the survival of fresh and frozen-thawed spermatozoa of the Angora goat. Aust J Biol Sci. 1982; 35:305-312. https://www.publish.csiro.au/bi/pdf/BI9820305
6. - Fathi M, Zaher R, Ragab D, Gamal I, Mohamed A, El Naga EA, et al. Soybean lecithin-based extender improves Damascus goat sperm cryopreservation and fertilizing potential following artificial insemination. Asian Pac J Reprod. 2019; 8(4):174-180. https://doi.org/10.4103/2305-0500.262834
7. - Alcay S, Toker MB, Gokce E, Onder NT, UstUner B, Nur Z. Long term incubation resilience of post-thaw ram semen diluted with lecithin-based extender supplemented with Bovine Serum Albumin. Kafkas Univ Vet Fak Derg. 2019; 25(3):291-297. https://doi.org/10.9775/kvfd.2018.20843
8. - Sandal AI, Senlikci H, Baran A, Ozdas OB. Effects of semen extender supplemented with Bovine Serum Albumin (BSA) on spermatological traits of Saanen buck semen stored at +4°C. Kafkas Univ Vet Fak Derg. 2020; 26(4):515-520. https://vetdergikafkas.org/uploads/pdf/pdf_KVFD_L_2696.pdf
9. - Software Statistical Minitab 19. State College, PA: Minitab, Inc. 2019.
10. - Osman CF, Nang SF, Ibrahim SB, Budin FHF, Jaffar NAA. Albumin improved spermatozoa quality and DNA integrity for freezing-free preservation. Int J Biol Med Res. 2012; 3(2):1670-1679. https://citeseerx.ist.psu.edu/viewdoc/download?doi=10.1.1.588.7002&rep=rep1&type=pdf
11. - Mahdi SAAW, Mahmood FA, Mahmoo RM. Effect of different concentrations of Bovine Serum Albumin on some of the frozen sperm characteristics of the rams. Plant Archives. 2019; 19(2):1486-1488. http://plantarchives.org/SPL%20ISSUE%20SUPP%202,2019/259%20(1486-1488).pdf
12. - Elliott GD, Wang S, Fuller BJ. Cryoprotectansa review of the actions and applications of cryoprotective solutes that modulate cell recovery from ultra-low temperatures. J Cryobiol. 2017; 70:74-91. https://doi.org/10.1016/j.cryobiol.2017.04.004
13. - Raju R, Bryant SJ, Wilkinson BL, Bryant G. The need for novel cryoprotectants and cryopreservation protocols: Insights into the importance of biophysical investigation and cell permeability. Biochim Biophys Acta Gen Subj. 2021; 1865:129749. https://doi.org/10.1016/j.bbagen.2020.129749
14. - Manjunath P. New insights into the understanding of the mechanism of sperm protection by extender components. Anim Reprod. 2012; 9(4):809-815. https://www.animal-reproduction.org/article/5b5a6053f7783717068b46cc/pdf/animreprod-9-4-809.pdf
15. - Pini T, Leahy T, de Graaf SP. Sublethal sperm freezing damage: Manifestations and solutions. Theriogenology. 2018; 118:172-181. https://doi.org/10.1016/j.theriogenology.2018.06.006
16. - Daramola JO. Effect of centrifugation on motility, sperm capacitation and acrosome reaction in soy bean and avocado seed milk extenders of cryopreserved goat spermatozoa. Agricultura Tropica Subtropica. 2017; 50(1):13-18. https://doi.org/10.1515/ats-2017-0002.
17. - Gojen L, Ray K, Sarkar B. Effect of different levels of egg yolk on cryopreservation of Black Bengal buck semen in Tris egg yolk citrate fructose glycerol extender. Iranian J of Applied Anim Sci. 2016; 6(1):101-106. http://ijas.iaurasht.ac.ir/article_520885.html
18. - Tabarez A, Garcia W, Palomo MJ. Effect of the type of egg yolk, removal of seminal plasma and donor age on buck sperm cryopreservation. Small Rumin. Res. 2017; 149:91-98. https://doi.org/10.1016/j.smallrumres.2017.01.007
19. - Ugur MR, Abdelrahman AS, Evans HC, Gilmore AA, Hitit M, Arifiantini RI, et al. Advances in cryopreservation of bull sperm. Front Vet Sci. 2019; 6:268. https://doi.org/10.3389/fvets.2019.00268
20. - Nethenzheni LP, Mphaphathi ML, Madzhie LR, Negota NC, Kalonji PVM, Nedambale TL, et al. Effect of Bioxcell® and Triladyl® extenders and removal of seminal plasma on equilibrated and cryopreserved semen from South African unimproved indigenous bucks. J Bacteriol Mycol. 2021; 8(1):1164 https://austinpublishinggroup.com/bacteriology/fulltext/bacteriology-v8-id1164.pdf
21. - Kopeika J, Thornhill A, Khalaf Y. The effect of cryopreservation on the genome of gametes and embryos: principles of cryobiology and critical appraisal of the evidence. Human Reproduction Update. 2015; 21(2):209-227. https://doi.org/10.1093/humupd/dmu063
22. - Zamiri MJ. Update on semen cryopreservation in sheep and goats: A review. J. Livest. Sci. Technol. 2020; 8(1):1-15. https://doi.org/10.22103/jlst.2020.15927.1321
23. - Baghel G, Anand M, Yadav S. Evaluate the effect of egg yolk and seminal plasma on spermatozoa abnormality using different cryopreservation protocol in Barbari buck semen. International J. Sci. Environ. Technol. 2016; 5(2):666-671. https://www.ijset.net/journal/916.pdf
24. - Tariq M, Khan MS, Shah MG, Nisha AR, Umer M, Hasan SM, et al. Exogenous antioxidants inclusion during semen cryopreservation of farm animals. J Chem Pharm Res. 2015; 7(3):2273-2280. https://www.researchgate.net/publication/281863711_Exogenous_antioxidants_inclusion_during_semen_cryopreservation_of_farm_animals.
25. - Sharma A, Sood P, Chaudhary JK. Correlation of climatic conditions with seminal quality parameters in Gaddi and Chegu buck semen. Asi J Anim Vet Adv. 2020; 15:32-37. https://doi.org/10.3923/ajava.2020.32.37
26. - Yodmingkwan P, Guntaprom S, Jaksamrit J, Lertchunhakiat K. Effects of Extenders on Fresh and Freezing Semen of Boer Goat. Agric Agric Sci Procedia. 2016; 11:125-130. https://doi.org/10.1016/j.aaspro.2016.12.021
27. - Narwade BM, Mohanty TK, Bhakat M, Rahim A. Goat semen cryopreservation using egg yolk and soya based extenders containing trehalose. India. J. Anim. Sci. 2017; 87(7):851-855. https://www.researchgate.net/publication/318860727_Goat_semen_cryopreservation_using_egg_yolk_and_soya_based_extenders_containing_trehalose#fullTextFileContent.
28. - Sharma A, Sood P, Chaudhary JK. Effect of different concentrations of glycerol in cryopreservation of Gaddi goat semen. J Vet Andr. 2020; 5(1):1-6 http://cesica.org/publicaciones/index.php/journal_veterinary_andrology/article/view/85/71
29. - Hezavehei M, Sharafi M, Kouchesfahani HM, Henkel R, Agarwal A, Esmaeili V, Shahverdi A. Sperm cryopreservation: A review on current molecular cryobiology and advanced approaches Boimed. 2018; 37(3):327-339. https://doi.org/10.1016/j.rbmo.2018.05.012
30. - Uysal O, Bucak MN. Effects of oxidized glutathione, bovine serum albumin, cysteine and lycopene on the quality of frozen-thawed ram semen. Acta Vet Brno. 2007; 76:383-390. https://doi.org/10.2754/avb200776030383