Gene expression of growth factor BMP15, GDF9, FGF2 and their receptors in bovine follicular cells

Expresión génica del factor de crecimiento BMP15, GDF9, FGF2 y sus receptores en células foliculares bovinas

Published 2018-09-01

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Reineri, P. S., Coria, M. S., Barrionuevo, M. G., Hernández, O., Callejas, S., & Palma, G. A. (2018). Gene expression of growth factor BMP15, GDF9, FGF2 and their receptors in bovine follicular cells. Journal MVZ Cordoba, 23(3), 6778-6787. https://doi.org/10.21897/rmvz.1367
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https://doi.org/10.21897/rmvz.1367
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Pablo S Reineri
Instituto Nacional de Tecnología Agropecuaria. Universidad Nacional de Santiago del Estero.
http://orcid.org/0000-0002-4325-3134

María S. Coria
Laboratorio de Producción Animal, INBIONATEC. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET).
http://orcid.org/0000-0002-3472-8775

María G. Barrionuevo
Laboratorio de Producción Animal, INBIONATEC. CONICET
http://orcid.org/0000-0002-9377-1060

Olegario Hernández
National Institute of Agricultural Technology, EEA Santiago del Estero, Santiago del Estero, Argentina.
http://orcid.org/0000-0001-6975-0254

Santiago Callejas
Reproduction Area, FISFARVET, CIVETAN, CONICET-CICPBA, National University of the Center of the Province of Buenos Aires (UNICEN), Tandil, Argentina.
http://orcid.org/0000-0002-9175-1322

Gustavo A. Palma
2National University of Santiago del Estero (UNSE), Santiago del Estero, Argentina. 3Animal Production Laboratory, INBIONATEC, Villa El Zanjón, Santiago del Estero, Argentina.
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Introduction. Growth and follicular maturation involve transformations of various components of the follicle, such as the oocyte, granulosa and techa cells. Several growth factors, including differentiation growth factor 9 (GDF9), bone morphogenic protein 15 (BMP15) and basic fibroblast growth factor (FGF2) are important for follicular development and oocyte maturation, by its ability to increase the proliferation of granulosa, techa cells and the ovarian stroma. Objetive. Evaluate mRNA expression of GDF9, BMP15, FGF2 and their main receptors, transforming growth factor beta receptor 1 (TGFβ-R1), bone morphogenetic protein receptor, type IB (BMPR-IB) and fibroblast growth factor receptor 2 (FGFR2) in bovine follicular cells. Materials and methods. Total RNA was isolated from pooled samples of oocytes (OOs), cumulus cells (CCs) of cumulus oocyte complexes (COCs) and follicular cell pellets (PCs) of 70 ovaries obtained from 96 beef heifers, collected at a local abattoir. The expression pattern of growth factors and their receptors in follicular bovine cells was evaluated by reverse transcriptase polymerase chain reaction (RT-PCR). Results. The mRNA transcripts encoding GDF9, BMP15, FGF2, TGFβ-R1, BMPR-IB and FGFR2 genes were detected, by RT-PCR, in all studied cells. This is the first time that the expression of TGFβ-R1 and BMPR-IB receptors is reported in bovine oocytes. Conclusions. The presence of growth factors and receptor transcripts in the studied cells indicate that these factors could act as paracrine and autocrine regulators of folliculogenesis.

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